Abstract:
:We compared physiological and ultrastructural indices of acute lung injury (ALI) during septic shock caused by taxonomically diverse pathogens to distinguish ALI due to endogenous inflammatory mediators vs. microbial exotoxins or other factors. Conscious rats were infected i.v. with gram-negative Escherichia coli (EC, serotype 055:B5), exotoxin-C producing gram-positive Staphylococcus aureus (SA), or yeast-phase Candida albicans (CA, a clinical isolate). Viable inocula of 10(10) EC, 10(10) SA, or 10(9) CA caused lethal shock in < 24 h, but distinct types of ALI were noted after bacteria vs. fungi. Within 0.5 h of EC infection, leukocytes marginated in the lung vasculature; by death at 6-14 h, animals were hyperoxemic but not acidemic, and showed slight interstitial edema with increased wet/dry weight ratios (W/D = 5.22 +/- 0.10, mean +/- SE, vs. 4.86 +/- 0.07 in controls, P < 0.05). Similarly mild ALI occurred after 10(10) SA. In contrast, within 0.5 h of CA infection, yeast were visible within lung intravascular leukocytes. By death at 6-12 h, CA animals showed hyperoxic acidemia and moderate ALI with capillary obstruction, interstitial hemorrhage, and elevated lung W/D (5.52 +/- 0.13, P < 0.01 vs. controls) associated with yeast-mycelial transformation. Prior neutropenia accelerated mortality and worsened ALI after CA, with hypoxemic acidemia, increased lung W/D (7.23 +/- 0.34, P < 0.05 vs. other groups), capillary occlusion, perivascular and alveolar hemorrhage, and septal disruption by mycelia. Bacteremia induced large increases in serum tumor necrosis factor-alpha (TNF) and interleukin-1 alpha within 1.5 h, but these cytokines remained low in CA animals, even at death. Neither survival nor ALI after EC or CA was altered by pentoxifylline, which attentuated TNF production, or by cyclooxygenase inhibition with ibuprofen. Thus, overall ALI severity correlated with physiological indices of pulmonary function, but ultrastructural changes correlated better with pathogen type than circulating cytokine or eicosanoid mediators. Whereas lethal bacteremia induced early cytokinemia and mild ALI with or without bacterial exotoxins, moderate ALI apparently was mediated by fungal exotoxins during lethal candidemia, which worsened during neutropenia due to enhanced mycelial proliferation.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Lechner AJ,Ryerse JS,Matuschak GMdoi
10.1002/jemt.1070260512subject
Has Abstractpub_date
1993-12-01 00:00:00pages
444-56issue
5eissn
1059-910Xissn
1097-0029journal_volume
26pub_type
杂志文章abstract::An energy-filtering transmission electron microscope with 300 kV acceleration voltage was developed and the spatial resolution of elemental distribution images was improved. Observing oxygen monolayers in Al(11)O(3)N(9), it was shown that the actual resolution attained is up to 0.5 nm. Surface plasmon loss images of s...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20025
更新日期:2004-02-15 00:00:00
abstract::Assessment of HER2/neu oncogene has been used as both a prognostic and predictive marker for breast cancer. However, the choice of the best method to assess the status of HER2/neu oncogene in breast cancer tissue remains controversial. A variety of techniques are available to detect HER2/neu gene amplification and ove...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10181
更新日期:2002-10-15 00:00:00
abstract::The morphology of pancreatic excretory duct segments was reviewed in mammals. The fine structure of the epithelial lining was described in intercalated ducts, intra- and extralobular ducts, and in major pancreatic ducts. Morphological characteristics of the various cell types comprising to the duct epithelium were det...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970601)37:5/6<407::AID-J
更新日期:1997-06-01 00:00:00
abstract::Experimentation has been carried out to study proliferation of plasma cells in the chicken Harderian gland (HG) and to determine if a HG factor influences immune cell (i.e., B cell) proliferation. In young chickens, flow cytometric analysis of propidium iodide (PI)-stained plasma cells revealed that the percentages of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19960601)34:2<149::AID-JEM
更新日期:1996-06-01 00:00:00
abstract::Adrenomedullin (AM) is a novel neuropeptide with special significance in the mammalian hypothalamo-hypophysial axis. By using an antiserum specific for human AM, we have studied the localization of AM-like immunoreactive (AMi) cell bodies and fibers in the hypothalamus and hypophysis of the amphibians Rana perezi (anu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1131
更新日期:2001-08-01 00:00:00
abstract::To evaluate the performance degradation of fabric drying in domestic air-vented dryer, the appearance, mechanical, and microscopic properties of the wool fabric after different drying cycles were studied. Pilling and dimensional stability tests were performed according to established international standards. Microscop...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22881
更新日期:2017-08-01 00:00:00
abstract::Voltage-gated sodium channels are largely localized to the nodes of Ranvier in myelinated axons, providing the physiological basis for saltatory conduction. Studies using antisodium channel antibodies have shown that along demyelinated axons sodium channels form new distributions. The nature of this changed distributi...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19960801)34:5<445::AID-JEM
更新日期:1996-08-01 00:00:00
abstract::Cigarette smoke is a significant source of cadmium, lead, and toxic elements, which are absorbed into the human organism. In this context, the aim of this study was to investigate in vitro the presence of toxic elements, cadmium, and lead deriving from cigarette smoke in the resin composite, dentine, and dental enamel...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20903
更新日期:2011-03-01 00:00:00
abstract::Vanda tessellata (Roxb.) Hook. ex G. Don. (grey orchid, family Orchidaceae) is an epiphytic orchid of horticultural importance and currently under threat due to overharvesting and habitat destruction. Micropropagation protocols were developed for the production of grey orchid but the survival success of in vitro regen...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23630
更新日期:2020-10-22 00:00:00
abstract::Iris L. is one of the important genus of family Iridaceae, consist of 56 taxa naturally occurred in Turkey. The similarities and variations in the subgenus overlapping the taxonomic positions of the species in the subgenera and needs anatomical assessment especially by microscopic techniques. In this study, the taxono...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23221
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Splint immobilization of the forearm is often performed in clinical practice. Previous studies investigated the effect of immobilization on bone, cartilage, muscle, and tendon, however, the acute effects on human skin microcirculation and histomorphology remains elusive. METHODS:In 12 healthy, nonsmoking in...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22317
更新日期:2014-01-01 00:00:00
abstract::The mammary adenocarcinoma cell line MTLn3 is chemotactic towards epidermal growth factor (EGF), and this induced motility is thought to promote breast cancer invasion and metastasis. Stimulation of MTLn3 cells with EGF results in the extension of a flat, thin structure filled with filamentous actin and termed a lamel...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19981201)43:5<433::AID-JEM
更新日期:1998-12-01 00:00:00
abstract::Protein expression patterns are a primary determinant of tissue function and in this study we developed methods to study protein expression over macroscopic distances at subcellular levels of detail. Using the mammalian lens as a model tissue system, we show that by combining two-photon microscopy with novel image mon...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10360
更新日期:2003-09-01 00:00:00
abstract::Programmed cell death occurs in a variety of organs during amphibian metamorphosis and is usually identified by electron microscopy as apoptosis or its modifications. Because of the massive cell death that occurs during a short period, amphibian organs serve as an ideal model system for the study of mechanisms underly...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19960615)34:3<228::AID-JEM
更新日期:1996-06-15 00:00:00
abstract::The wavelength of Raman-scattered light depends on the molecular composition of the substance. This is the first attempt to acquire Raman spectra of a mouse eyeball removed from a living mouse, in which the eyeball was preserved using the "in vivo cryotechnique" followed by freeze-drying. Eyeballs were cryofixed using...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20449
更新日期:2007-07-01 00:00:00
abstract::Acquisition of a great number of energy-filtered images in a TEM (EFTEM) around the characteristic signal with a low energy-selecting slit allows display of the electron energy loss (EEL)-spectrum of regions of interest (ROIs) of a sample. These EEL-spectra can be submitted to the different treatments already in use f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1079
更新日期:2001-04-15 00:00:00
abstract::High speed data registration is required for the study of fluorescence resonance energy transfer in real time as well as fast dynamic intra- and inter-cellular signaling events. Multispectral confocal spinning disk microscopy provides a high resolution method for performing such real time live cell imaging. However, o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20423
更新日期:2007-04-01 00:00:00
abstract::The ultrastructure of adult human articular cartilage matrix is reexamined in tissue processed according to recently improved cryotechniques [Studer et al. (1995) J. Microsc., 179:321-332]. In truely vitrified tissue, a network of fine cross-banded filaments (10-15 nm in diameter) with a periodicity characteristic of ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970515)37:4<271::AID-JEM
更新日期:1997-05-15 00:00:00
abstract::Myotonic dystrophy (DM) is an inherited multisystem disorder characterized by the presence of a high polymorphic expansion of trinucleotide (CTG) repeat in the 3' untranslated region of the DM protein kinase (DMPK) gene. However, the role of myotonic dystrophy protein kinase (DMPK) has yet to be elucidated. Studies ai...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10223
更新日期:2002-09-01 00:00:00
abstract::Human monocytic leukemia U937 cells readily undergo apoptosis when exposed to various stimuli, including inhibition of protein synthesis, oxidative stress, antitumoral agents, etc. The sequential, step-by-step morphological changes in U937 cells that occur during the apoptotic program are largely determined by the act...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20058
更新日期:2004-05-01 00:00:00
abstract::Number of molecules and Brightness (N&B) has been proposed for measuring the molecular brightness and number of fluorophores in time-sequence of images, in live cells. If the fluorescently tagged-proteins are mobile in the illumination volume, the stoichiometry of their oligomers can be derived from the increase of th...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22277
更新日期:2013-11-01 00:00:00
abstract::Antlion (Myrmeleon sagax) larvae live in sandy soil and possess the ability to enter soil quickly. In this article, the hierarchical structure of the nonsmooth surface of antlion larvae was obtained using a scanning electron microscope (SEM). Based on the results, a bionic nonsmooth structure model was established to ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23419
更新日期:2020-04-01 00:00:00
abstract::We summarize the molecular phenotype, diagnostic criteria, and the newest advances related to seeking the pathogenic mechanism(s) of sporadic inclusion-body myositis (s-IBM), a muscle disease usually of persons over age 50. On the basis of our research, several processes seem to be important in relation to the still-s...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20186
更新日期:2005-07-01 00:00:00
abstract::Regeneration of skeletal parts of fossil echinoderms is reviewed. Among regenerations of various fossil echinoderms, those of crinoids are most common and many examples have been reported. Mesozoic and Cenozoic crinoids, the subclass Articulata, are characterized by the dominance of muscular articulations in the arms ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1186
更新日期:2001-12-15 00:00:00
abstract::Research into the POU transcription factor Oct-6 has been the focus of much current attention, in particular its role in Schwann cell development and differentiation. Based on published data and data presented here, we propose a model for Oct-6 function at two distinct stages of Schwann cell maturation. First, Oct-6 f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980601)41:5<372::AID-JEM
更新日期:1998-06-01 00:00:00
abstract::An overview of the morphological correlates of mammalian epidermal lipids and the cutaneous permeability barrier is provided. The following features are discussed: 1) ultrastructural characterization of the progressive lipid alterations accompanying normal stratum corneum (SC) formation; 2) effects of drugs/inhibitors...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970501)37:3<180::AID-JEM
更新日期:1997-05-01 00:00:00
abstract::Peroxisomes are essential organelles that may be involved in various functions, dependent on organism, cell type, developmental stage of the cell, and the environment. Until recently, peroxisomes were viewed as a class of static organelles that developed by growth and fission from pre-existing organelles. Recent obser...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10323
更新日期:2003-06-01 00:00:00
abstract::Currently, composite materials dominate among the restorative materials used for direct aesthetic filling. Reinforcement of composites with glass fibers allows for the transfer of greater loads and better durability between the tooth tissue and the filling polymer. New approach to bonding liquid materials with composi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23700
更新日期:2021-01-16 00:00:00
abstract::This study was carried out to identify 20 high valued medicinal plants of District Lahore, Punjab, Pakistan with the help of the palynological studies. The distinguishing characteristics for selected species were found by systematized methods using light microscopy and Scanning Electron Microscopy. In this study, impo...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23096
更新日期:2018-12-01 00:00:00
abstract::The aim was to assess the effects of 1% peracetic acid (PAA) as a single endodontic irrigant on microhardness, roughness, and erosion of root canal dentin, compared with 2.5% sodium hypochlorite (NaOCl) and with 2.5% NaOCl combined with 17% EDTA. Forty human, single-rooted tooth hemisections were submitted to Knoop mi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23424
更新日期:2020-04-01 00:00:00