Abstract:
:Numerous highly fluorescent macrophages (MPhi), designated "smoker cells," exist in the lungs of smokers and subjects who have quit smoking within 5 years. The brightly fluorescent MPhi, however, are not present in the lungs of never smokers. Some investigators have speculated that the intense fluorescence of the MPhi is due to smoke-induced changes in the autofluorescence of naturally occurring (i.e., endogenous) compounds (e.g., NADP). In contrast, other researchers have theorized that the fluorescence is due to the uptake of tobacco smoke particulates (i.e., "tar"). Studies reported herein were undertaken to test the hypothesis that the origin of the MPhi fluorescence could be profiled with the novel technologies afforded by spectral confocal laser scanning microscopy (sCLSM) and multispectral cytometry (MSC). To this end, spectral emissions were obtained by sCLSM of optical sections of live MPhi isolated from fresh surgically excised human lung tissue and in air-dried lung tissue imprints. Confirmation of spectral profiles of these single cell observations was obtained in population studies with the use of high-throughput MSC in which multispectral analyses were performed with three different lasers. Proof of concept experiments demonstrated that relatively nonfluorescent MPhi from the lungs of nonsmokers became fluorescent upon short-term ex vivo exposure to tobacco smoke tar. Summarily, the studies reported herein document that the fluorescence of human lung MPhi is due to tobacco tar.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Pauly JL,Allison EM,Hurley EL,Nwogu CE,Wallace PK,Paszkiewicz GMdoi
10.1002/jemt.20191keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
79-89issue
2eissn
1059-910Xissn
1097-0029journal_volume
67pub_type
杂志文章abstract::In a previous report we described the survival and contractile function of mouse embryonic stem cell-derived cardiomyocytes in the host retroperitoneum. To further understand the nature of embryonic stem cell-derived cardiomyocytes, the study assessed the synthesis of natriuretic peptides in ectopic myocardial tissues...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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abstract::The human dentin-enamel junction (DEJ) is a natural junction that unites two dissimilar mineralized tissues in the human tooth: enamel and dentin. DEJ plays a critical role in maintaining structural and functional integrity of the tooth. However, its structure, chemical composition and function remain unclear and cont...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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pub_type: 杂志文章,多中心研究
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
pub_type: 杂志文章
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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doi:10.1002/jemt.20664
更新日期:2009-03-01 00:00:00
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journal_title:Microscopy research and technique
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doi:10.1002/jemt.20495
更新日期:2007-10-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22539
更新日期:2015-08-01 00:00:00
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journal_title:Microscopy research and technique
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doi:10.1002/jemt.20078
更新日期:2004-06-01 00:00:00
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
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abstract::Substance P (SP) after binding to the neurokinin-1 (NK-1) receptor regulates many biological functions. Both SP and the NK-1 receptor are expressed in human normal placenta cells, monocytes, and macrophages. However, to our knowledge, the presence of both SP and the NK-1 receptor in macrophages of the placenta, the Ho...
journal_title:Microscopy research and technique
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journal_title:Microscopy research and technique
pub_type: 历史文章,杂志文章
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abstract::Scanning electron microscope images of complete and damaged frustules of Nitzschia marina Grun. revealed an unusual chambered structure of the valve. This structure is not present in any existing nitzschioid genus and warrants an independent generic status for this taxon, which is therefore separated into Alveus gen. ...
journal_title:Microscopy research and technique
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abstract::Programmed cell death occurs in a variety of organs during amphibian metamorphosis and is usually identified by electron microscopy as apoptosis or its modifications. Because of the massive cell death that occurs during a short period, amphibian organs serve as an ideal model system for the study of mechanisms underly...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
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abstract::In order to investigate the mode of existence of the sarcoglycan complex, neuronal nitric oxide synthase (nNOS), beta-dystroglycan, and dystrophin in the normal skeletal myofiber, we examined the ultrastructural localization and mutual spatial relationship of nNOS, beta-dystroglycan, dystrophin, and the individual com...
journal_title:Microscopy research and technique
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abstract::Chironomids have been widely used in environmental monitoring and toxicity assays. Assessment criteria for chironomids range from the molecular-biochemical level to the population level. However, the use of markers to evaluate cellular and anatomical changes in organs and systems of individuals under contaminant expos...
journal_title:Microscopy research and technique
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doi:10.1002/jemt.23278
更新日期:2019-08-01 00:00:00
abstract::Intramandibular glands have been poorly studied in polymorphic ants, where the differences between castes were unsufficiently scrutinized. Leaf-cutting ants possess one of the most complex systems of communication and labor division, which is polymorphic well as age polyethism, and makes them an ideal model for the st...
journal_title:Microscopy research and technique
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更新日期:2015-07-01 00:00:00