Abstract:
:Ultra-thin frozen sections are ideal substrates for immunolabelling in high resolution electron microscopy. However, visualization of subcellular structures is inferior to that obtained with corresponding plastic sections. Although negative staining is generally effective and even superior to positive staining, the accumulated stain is often too heavy, obscuring morphology and markers used for immunocytochemical localization of antigens. This paper describes the development of a modified negative contrast staining technique in which a high concentration of uranyl acetate is mixed with methyl cellulose at a low pH. Application of this stain to cryosections of cells and tissue resulted in improved visualization of morphological structures characterized by negative images of membranes and cell organelles. Use of this stain is advantageous for morphological and immunocytochemical studies involving ultra-thin frozen sections.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Himmelhoch Sdoi
10.1002/jemt.1070290104subject
Has Abstractpub_date
1994-09-01 00:00:00pages
23-8issue
1eissn
1059-910Xissn
1097-0029journal_volume
29pub_type
杂志文章abstract::In this paper we report the effect of noble gas ions bombardment on the degradation of atomically flat Si(111) surfaces at room and high (400 degrees C-600 degrees C) temperatures. Reflection high energy electron diffraction (RHEED) and reflection electron microscopy (REM) have been used to characterize the topography...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200406
更新日期:1992-02-15 00:00:00
abstract::Rhus succedanea is generally traded, distributed, and sold in the markets in its crude and raw form. This may have been mixed with adulterants, mismanaged by malpractices, and substituted with other closely related drugs having different effect. This study is therefore carried out to authenticate the plant through pha...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23367
更新日期:2019-12-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23118
更新日期:2018-10-01 00:00:00
abstract::GABA is a likely feedback transmitter from H1 horizontal cells to cone photoreceptors in fish retinas. Spinules arise from H1 cell dendrites in light-adapted retinas, are correlated with responses attributed to feedback, and have been proposed to be the GABA release sites. We used mAb 62-3G1, an antibody against the b...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970101)36:1<43::AID-JEMT
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:The use of quality control tool for adulteration of Senna (Cassia aungustifolia) a pharmaceutically very important. They were used for multiple health disorders such as constipation, indigestion, epilepsy, asthma, piles, migraine, and heart problems. Two different species of same family or same genus used co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23332
更新日期:2019-10-01 00:00:00
abstract::STM tips made from antiferromagnetic MnNi have been used to investigate the atomic structure of the (001) and (111) surfaces of Fe3O4. The clean (001) surface displays a ( radical2 x radical2)R45 degrees superlattice, which is attributed to charge-ordering on the surface, where Fe(2+)-Fe2+ and Fe(3+)-Fe3+ dimers can b...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20148
更新日期:2005-02-01 00:00:00
abstract::Transgenic mice are valuable experimental models of human endocrine diseases. Targeted ablation of specific cell lineages or insertion of genes coding for releasing factors, hormones, growth factors, and oncogenes fused with appropriate promoters, or mutated genes, can induce several pituitary disorders. Various hypos...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19971015)39:2<194::AID-JEM
更新日期:1997-10-15 00:00:00
abstract::We compared physiological and ultrastructural indices of acute lung injury (ALI) during septic shock caused by taxonomically diverse pathogens to distinguish ALI due to endogenous inflammatory mediators vs. microbial exotoxins or other factors. Conscious rats were infected i.v. with gram-negative Escherichia coli (EC,...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260512
更新日期:1993-12-01 00:00:00
abstract::Automated detection and classification of gastric infections (i.e., ulcer, polyp, esophagitis, and bleeding) through wireless capsule endoscopy (WCE) is still a key challenge. Doctors can identify these endoscopic diseases by using the computer-aided diagnostic (CAD) systems. In this article, a new fully automated sys...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23447
更新日期:2020-05-01 00:00:00
abstract::The rapid photothermal lysis of Escherichia coli O157:H7 treated with light-triggered Janus nanomotors was visualized by Hilbert differential contrast transmission electron microscopy (HDC-TEM). The extraordinary advantage of this high-resolution microscopic technique was that it revealed the detailed ultrastructure a...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23657
更新日期:2020-11-27 00:00:00
abstract::This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan. The pollen morphology of 10 species of the family Apocynaceae was observed and documented using light microscopy and scanning electron microscopy. Pollen was found subspheroidal...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23293
更新日期:2019-09-01 00:00:00
abstract::Bats are the only mammals that can fly in the dark without eye usage. This study was conducted to describe the structural and functional adaptations of the retina of two bats very common in the Egyptian fauna having a different lifestyle: the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrell...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23530
更新日期:2020-11-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.20672
更新日期:2009-03-01 00:00:00
abstract::An overview of the morphological correlates of mammalian epidermal lipids and the cutaneous permeability barrier is provided. The following features are discussed: 1) ultrastructural characterization of the progressive lipid alterations accompanying normal stratum corneum (SC) formation; 2) effects of drugs/inhibitors...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970501)37:3<180::AID-JEM
更新日期:1997-05-01 00:00:00
abstract::This experiment was conducted in Ile-de-France adult rams to examine the target point of a 2-month light cycle regimen on seminiferous tubule functions, on intertubular compartment and on Leydig cell parameters. Eight rams were subjected to a 2-month light cycle regimen and were compared to sexually active or inactive...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200306
更新日期:1992-02-01 00:00:00
abstract::Cigarette smoke is a significant source of cadmium, lead, and toxic elements, which are absorbed into the human organism. In this context, the aim of this study was to investigate in vitro the presence of toxic elements, cadmium, and lead deriving from cigarette smoke in the resin composite, dentine, and dental enamel...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20903
更新日期:2011-03-01 00:00:00
abstract::We have used our opossum model of fetal nephrotoxicity to investigate uranyl nitrate-induced morphologic changes in the developing kidney. The present study establishes a renal dose response curve for the uranyl nitrate (UN). We find that pups treated with nonlethal doses of UN do not demonstrate growth retardation co...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19971101)39:3<285::AID-JEM
更新日期:1997-11-01 00:00:00
abstract::Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290404
更新日期:1994-11-01 00:00:00
abstract::We present a microscopic investigation of nanocomposite films of iron oxide (γ-Fe(2)O(3)) magnetic nanowires (NWs) aligned into polymers, formed upon evaporation of solutions of acrylate polymer/magnetic nanoparticles under magnetic field (MF). The field causes the assembly of the γ-Fe(2)O(3) nanoparticles along the d...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20848
更新日期:2010-10-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22317
更新日期:2014-01-01 00:00:00
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journal_title:Microscopy research and technique
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更新日期:2012-10-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23278
更新日期:2019-08-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22273
更新日期:2013-11-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20832
更新日期:2010-10-01 00:00:00
abstract::In the current investigation, 16 samples of six species of the genus Polygonatum were collected from different geographical regions in China. Leaf micromorphological traits were studied in detail using scanning electron microscopy. About five-leaf samples were used for each species collected from different geographica...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23529
更新日期:2020-11-01 00:00:00
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journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070230202
更新日期:1992-10-15 00:00:00
abstract::The present study provides experimental data which indicate that the neutrophil is ideal for studying programmed cell death or apoptosis in vitro. Neutrophils can be obtained from human peripheral blood in large numbers with minimal experimental manipulation and are easily separated from other leukocytes, providing ne...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070280408
更新日期:1994-07-01 00:00:00
abstract::This article presents an integrated approach for the authentication of five Russian icons through the study of their paint materials (elemental composition and stratigraphic mapping), state of conservation and artistic technique. The results of the applied analytical protocol, involving optical and scanning electron m...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20727
更新日期:2009-10-01 00:00:00
abstract::Shape from focus (SFF) is a widely used passive optical method for 3D shape reconstruction. In SFF, a focus measure, which is used to estimate the relative focus level, plays a critical role in depth estimation. In this article, we present a new focus measure for accurate 3D shape estimation in optical microscopy base...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23386
更新日期:2020-01-01 00:00:00
abstract::The mammary adenocarcinoma cell line MTLn3 is chemotactic towards epidermal growth factor (EGF), and this induced motility is thought to promote breast cancer invasion and metastasis. Stimulation of MTLn3 cells with EGF results in the extension of a flat, thin structure filled with filamentous actin and termed a lamel...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19981201)43:5<433::AID-JEM
更新日期:1998-12-01 00:00:00