Abstract:
:The mechanism of the candidacidal activity of histatins 3 and 5 (Hst) is still a matter of debate. Previous studies have indicated that Hst induce cell permeabilization, generation of reactive oxygen species (ROS) by mitochondria, inhibition of the respiratory chain, and energy-dependent cytotoxic release of ATP. On the other hand, the multiplicity of effects and the apparent contrast between experimental data continue to render the mechanism of Hst-induced killing of C. albicans unclear. In this investigation, using fluorescent probes (the potential-sensitive mitochondrial probe tetramethylrhodamine methyl ester perchlorate, TMRM; the ROS-sensitive probe dihydrofluorescein diacetate, DHF; the membrane-impermeant probe, calcein) and autofluorescence data we observed that Hst induce ROS generation by mitochondria undergoing a high energy swelling condition, accompanied by oxidation of cytosolic NAD(P)H and mitochondrial flavoproteins. ROS generation and swelling, attributable to an inhibition of the respiratory chain and to impairment of the K/H-exchanger, were followed by mitochondrial depolarization. Mitochondrial changes were accompanied by massive calcein influx, indicative of cell permeabilization, and prominent alterations of the cell size, shape, and optical density. The loss of proliferative activity was correlated, on a single cell basis, to the acquisition of a lipofuscin-like autofluorescence.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Diaz G,Polonelli L,Conti S,Messana I,Cabras T,Putzolu M,Falchi AM,Fadda ME,Cosentino S,Isola Rdoi
10.1002/jemt.20161keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
219-28issue
5eissn
1059-910Xissn
1097-0029journal_volume
66pub_type
杂志文章abstract::This article focusses on the structural development of human esophageal ciliated epithelium. A combination of transmission electron microscopic (TEM), scanning electron microscopic (SEM), radioautographic, and light microscopic (LM) analyses were carried out using intact fetal tissues between 8 and 20 weeks of gestati...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310305
更新日期:1995-06-15 00:00:00
abstract::Mammalian choroid plexuses (CPs) are vascularized structures involved in numerous exchange processes that supply nutrients and hormones to the brain, and that remove deleterious compounds and metabolites from the brain. Studies in the adult Mediterranean buffalo have investigated the morphology of CPs using histochemi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22037
更新日期:2012-08-01 00:00:00
abstract::Dystrophin-deficient muscles have repeated cycles of necrosis and regeneration, being susceptible to injury induced by muscle contractions. Some studies have demonstrated that tendons are also affected in mdx mice, based especially on the changes in biomechanical properties arising from the respective linked muscles. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22448
更新日期:2015-01-01 00:00:00
abstract::The superior olivary complex (SOC) is part of the auditory brainstem of the vertebrate brain. Residing ventrally in the rhombencephalon, it receives sensory signals from both cochleae through multisynaptic pathways. Neurons of the SOC are also a target of bilateral descending projections. Ascending and descending effe...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20001115)51:4<364::AID-JEMT6>3.0
更新日期:2000-11-15 00:00:00
abstract::The neurotrophins are a diverse family of peptides which activate specific tyrosine kinase-linked receptors. Over the past five decades, since the pioneering work of Levi-Montalcini and colleagues, the critical role that neurotrophins play in shaping the developing nervous system has become increasingly established. T...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990515/01)45:4/5<276::AI
更新日期:1999-05-15 00:00:00
abstract::Linepithema micans Forel is a poorly studied species that is now considered a pest in vineyards in South Brazil. Larval characters have been used in a few phylogenetic studies, and their importance greatly depends in the amount of available information on different species. This study presents a complete panorama on t...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20913
更新日期:2011-04-01 00:00:00
abstract::The field of quantitative analysis and subsequent mapping of the cerebral cortex has developed rapidly. New powerful tools have been applied to investigate large regions of complex folded gyrencephalic cortices in order to detect structural transition regions that might partition different cortical fields of disjunct ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20033
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:Ultrasonic excitation (US) was applied to glass ionomer cement (GIC) during early set time to increase the advantageous properties of this material. PURPOSE:The aim of this in vitro study was to assess the inner porosity of GIC after US. STUDY DESIGN:A total of 16 specimens, for each material, were prepare...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20873
更新日期:2011-01-01 00:00:00
abstract::The carotid body (CB) is a highly specialized small organ located at the bifurcation of the common carotid artery in the neck and plays an important role in acute adaptation to hypoxia. The most common diseased state of the carotid body is its enlargement (i.e., the CB paraganglioma), which can be caused by a genetic ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10200
更新日期:2002-11-01 00:00:00
abstract::All termite species (Isoptera) are eusocial. These insects live in societies where the division of labor is a predominant characteristic, conditioned by the presence of castes with different morphology, ontogeny, and development. The soldier caste is unique among social insects and it is responsible for colony defense...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20365
更新日期:2006-11-01 00:00:00
abstract::Growth rings were used to determine the root age of medicinal Paeonia lactiflora from four producing areas, and their corresponding paeoniflorin content were measured based on the identification of ages. Different P. lactiflora root samples of different ages were collected from the four major growing areas in China: B...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22048
更新日期:2012-09-01 00:00:00
abstract::The process of making of flexographic printing plate by the digital method proceeds in the following stages: back exposure, laser irradiation, main exposure, washing, post exposure, and finishing of the photopolymer plate. Laser irradiation is one of the basic technological stages, in which a negative of the reproduce...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.22418
更新日期:2016-11-01 00:00:00
abstract::Alcian blue (AB) and ruthenium red (RR) effects on ultrastructural preservation of the bacterial cell envelope of methanotrophs are compared. A previous successful method with RR that enhanced preservation of outer envelope layers in two representative methanotroph species is applied to other genera and species of met...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200109
更新日期:1992-01-01 00:00:00
abstract::This report describes osteoclastic bone resorption around intraosseous fixation screws in rat and pig mandibles. These screws supported distraction devices and provided for neutral fixation following the distraction period. Progressive clinical instability of bicortical screws and radiographic and histologic evidence ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10376
更新日期:2003-08-15 00:00:00
abstract::Neuroblast pattern, engrailed expression and proliferation in the subesophageal neuromers of the beetle Tenebrio molitor are characterized throughout embryogenesis. The proliferation of neuroblasts has been studied throughout postembyronic development. Serotonin, crustacean cardioactive peptide and tyrosine hydroxylas...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19961015)35:3<180::AID-JEM
更新日期:1996-10-15 00:00:00
abstract::The discovery of new song types in Hawaiian Drosophila species has raised a question of alternative sound production and sound perception mechanisms in Drosophila. For example D. disjuncta males, which produce song with a carrier frequency of 5,000-6,000 Hz, do not vibrate their wings but only tremble them in a very s...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20000715)50:2<161::AID-JEMT8>3.0
更新日期:2000-07-15 00:00:00
abstract::This experiment was conducted in Ile-de-France adult rams to examine the target point of a 2-month light cycle regimen on seminiferous tubule functions, on intertubular compartment and on Leydig cell parameters. Eight rams were subjected to a 2-month light cycle regimen and were compared to sexually active or inactive...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200306
更新日期:1992-02-01 00:00:00
abstract::The glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicul...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22040
更新日期:2012-08-01 00:00:00
abstract::The cyclic fluctuations in circulating levels of 17 beta-estradiol and progesterone that occur during the menstrual or estrous cycle are responsible for dramatic, cyclic changes in the epithelial lining and secretory status of the mammalian oviduct. The timely transition in the synthesis and release of oviduct protein...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070310606
更新日期:1995-08-15 00:00:00
abstract::Basement membranes (BMs) were first described in the mid-19th century, but they were not isolated and prepared for compositional studies until nearly 100 years later. Early methods of isolation were carried out on renal glomeruli, which were first sub-fractionated from kidney tissues by sieving. BMs were then isolated...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070280302
更新日期:1994-06-15 00:00:00
abstract::Fluorescence correlation spectroscopy (FCS) is a very sensitive technique that can be used, e.g., for the measurement of low concentrations and for the investigation of transport of fluorescent molecules. Fluorescence lifetime imaging (FLIM) provides spatially resolved information about molecular fluorescence lifetime...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20428
更新日期:2007-05-01 00:00:00
abstract::Scanning electron microscope images of complete and damaged frustules of Nitzschia marina Grun. revealed an unusual chambered structure of the valve. This structure is not present in any existing nitzschioid genus and warrants an independent generic status for this taxon, which is therefore separated into Alveus gen. ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(199601)33:1<2::AID-JEMT2>3
更新日期:1996-01-01 00:00:00
abstract::The flower buds of five Lonicera species, Lonicera japonica Thunb., L. macranthoides Hand.-Mazz., L. hypoglauca Miq., L. confusa DC. and L. fulvotomentosa Hsu et S.C. Cheng are confusable and usually utilized under the same name "Jinyinhua" in different areas for morphological similarity. Studies found that these five...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20882
更新日期:2011-02-01 00:00:00
abstract::The properties of the postganglionic sympathetic neurones supplying the heart and arising in the stellate and adjacent paravertebral ganglia of various species are discussed with respect to their location, morphology, synaptic input and membrane characteristics. Results from our laboratory on the morphology of rat ste...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19960901)35:1<69::AID-JEMT
更新日期:1996-09-01 00:00:00
abstract::This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan. The pollen morphology of 10 species of the family Apocynaceae was observed and documented using light microscopy and scanning electron microscopy. Pollen was found subspheroidal...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23293
更新日期:2019-09-01 00:00:00
abstract::Frozen skin sections are routinely used for light microscopic immunohistochemical study of the skin basement membrane zone for two reasons: some skin basement membrane zone proteins are labile to routine chemical fixation, and skin is not amenable to vibratome sectioning. However, inherent limitations of conventional ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1136
更新日期:2001-08-15 00:00:00
abstract::In this study we report for the first time the localization of a photoreceptor pigment in the cilia of the colored heterotrich ciliates Blepharisma japonicum red and blue form, Fabrea salina, and Stentor coeruleus, as result of a confocal microscopy investigation. Optical sectioning confocal microscopy has been used f...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20508
更新日期:2007-12-01 00:00:00
abstract::A variety of intracellular recording and staining techniques has been used to establish structure-function and, in some cases, structure-function-neurochemical correlations in fish, turtle, and cat retinae. Cone photoreceptor-horizontal cell connectivity has been studied extensively in the cyprinid fish retina by intr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070240106
更新日期:1993-01-01 00:00:00
abstract::To investigate intrinsic physicochemical properties and interactions of three different calcium hydroxide-based medications via means of different analytical methods. Two-commercial premixed medications: TempCanal(TCmx) and ProCalR(PCmx) and powder-form ProCal(PCpw) with glycerin were used. Vibrational modes were anal...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23600
更新日期:2020-09-21 00:00:00
abstract::STM tips made from antiferromagnetic MnNi have been used to investigate the atomic structure of the (001) and (111) surfaces of Fe3O4. The clean (001) surface displays a ( radical2 x radical2)R45 degrees superlattice, which is attributed to charge-ordering on the surface, where Fe(2+)-Fe2+ and Fe(3+)-Fe3+ dimers can b...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20148
更新日期:2005-02-01 00:00:00