Abstract:
:Directed growth cone movement is crucial for the correct wiring of the nervous system. This movement is governed by the concerted actions of cell surface receptors, signaling proteins, cytoskeleton-associated molecules, and molecular motors. In order to investigate the molecular basis of growth cone motility, we applied a new technique to functionally inactivate proteins: micro-scale Chromophore-Assisted Laser Inactivation [Diamond et al. (1993) Neuron 11:409-421]. Micro-CALI uses laser light of 620 nm, focused through microscope optics into a 10-microm spot. The laser energy is targeted via specific Malachite green-labeled, non-function-blocking antibodies, that generate short-lived protein-damaging hydroxyl radicals [Liao et al. (1994) Proc Natl Acad Sci USA 91:2659-2663]. Micro-CALI mediates specific loss of protein function with unachieved spatial and temporal resolution. Combined with time-lapse video microscopy, it offers the possibility to induce and observe changes in growth cone dynamics on a real time base. We present here the effects of the acute and localized inactivation of selected growth cone molecules on growth cone behavior and morphology. Based on our observations, we propose specific roles for these proteins in growth cone motility and neurite outgrowth.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Buchstaller A,Jay DGdoi
10.1002/(SICI)1097-0029(20000115)48:2<97::AID-JEMTkeywords:
subject
Has Abstractpub_date
2000-01-15 00:00:00pages
97-106issue
2eissn
1059-910Xissn
1097-0029pii
10.1002/(SICI)1097-0029(20000115)48:2<97::AID-JEMTjournal_volume
48pub_type
杂志文章,评审abstract::Atomic force microscopy has been used to study self-assembled structures of two alpha-hemolysin mutants. For a mutant (alphaHL-H5) that was locked into the prepore state on fluid phase egg-PC membranes, we visualized, for the first time, heptameric prepores and showed that the 7-fold axis in the prepore lies perpendic...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19990301)44:5<353::AID-JEM
更新日期:1999-03-01 00:00:00
abstract::The neurotrophins are a diverse family of peptides which activate specific tyrosine kinase-linked receptors. Over the past five decades, since the pioneering work of Levi-Montalcini and colleagues, the critical role that neurotrophins play in shaping the developing nervous system has become increasingly established. T...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19990515/01)45:4/5<276::AI
更新日期:1999-05-15 00:00:00
abstract::Locusts are attractive model preparations for cellular investigations of neurodevelopment. In this study, we investigate the immunocytochemical localization of histamine in the developing ventral nerve cord of two locust species, Schistocerca gregaria and Locusta migratoria. Histamine is the fast neurotransmitter of p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20980
更新日期:2011-10-01 00:00:00
abstract::Number of molecules and Brightness (N&B) has been proposed for measuring the molecular brightness and number of fluorophores in time-sequence of images, in live cells. If the fluorescently tagged-proteins are mobile in the illumination volume, the stoichiometry of their oligomers can be derived from the increase of th...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22277
更新日期:2013-11-01 00:00:00
abstract::Anastatus japonicus Ashmead (Hymenoptera: Eupelmidae) is an important egg parasitoid of several major insect pests. To better understand its host finding mechanisms, the antennal sensilla of female wasp were investigated by scanning electron microscopy. Sensilla chaetica were found mainly on radicle and pedicel segmen...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22032
更新日期:2012-08-01 00:00:00
abstract::Studies in insect gustation have a long history in general physiology, particularly with work on fly labellar and tarsal sensilla and in the general field of insect-plant interactions, where work on immature Lepidoptera and chrysomelid beetles has been prominent. Much more emphasis has been placed on the physiological...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991215)47:6<401::AID-JEM
更新日期:1999-12-15 00:00:00
abstract::Neuroblast pattern, engrailed expression and proliferation in the subesophageal neuromers of the beetle Tenebrio molitor are characterized throughout embryogenesis. The proliferation of neuroblasts has been studied throughout postembyronic development. Serotonin, crustacean cardioactive peptide and tyrosine hydroxylas...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19961015)35:3<180::AID-JEM
更新日期:1996-10-15 00:00:00
abstract::The present study investigated the regenerative potential of connective tissues harvested from two palatal areas widely used as donor sites for muco-gingival surgical approaches. Connective tissue grafts (CTGs) were obtained by de-epithelialisation of a free gingival graft (deCTG) and by a split flap approach from a p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22925
更新日期:2017-12-01 00:00:00
abstract::High speed data registration is required for the study of fluorescence resonance energy transfer in real time as well as fast dynamic intra- and inter-cellular signaling events. Multispectral confocal spinning disk microscopy provides a high resolution method for performing such real time live cell imaging. However, o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20423
更新日期:2007-04-01 00:00:00
abstract::This review article focuses on the ontogeny and the regulatory mechanisms involved in the modulation of the intracellular events governing the assembly and delivery of lipoproteins in human gut. The human fetal intestine organizes villi covered with well-differentiated enterocytes during the end of the first trimester...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000515)49:4<363::AID-JEM
更新日期:2000-05-15 00:00:00
abstract::The ruminant placenta has a very uniform gross structure based on localised areas of fetomaternal membrane apposition and proliferation to form placentomes. There is no consistency, however, in the number or size of these placentomes (6-150), nor in the villus architecture of the individual placentome. The one consist...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970701/15)38:1/2<88::AID
更新日期:1997-07-01 00:00:00
abstract::The antennal sensilla of the pine weevil (Pissodes nitidus Roel.) were observed with scanning electron microscopy and transmission electron microscopy (TEM). The weevil antenna consists of a long scape, a pedicel, and a flagellum with 10 segments; the last four flagellum segments are fused, forming the antennal club, ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20920
更新日期:2011-05-01 00:00:00
abstract::Because of the morphological and macroscopic similarity, many species of Erigeron and Aster (Asteraceae) are confusable and usually used under the same name "Meiduoluomi" in traditional Tibetan medicine (TTM). To find an easy, quick, and reliable method to authenticate and distinguish the eight main medicinal plants o...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20725
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVES:This study investigated the effects of sliding on the ultrastructure of three representative esthetic superelastic 0.014 inch nickel-titanium (NiTi) archwires. METHODS:Atomic force microscopy, scanning electron microscopy, and light microscopy were used to estimate the surface roughness of archwires and bra...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22557
更新日期:2015-10-01 00:00:00
abstract::In this study, we aimed to increase the sensitivity of protein labeling using 1.4 nm gold nanoparticles and glutamate δ2 receptor (GluD2) from the postsynaptic membrane of the Purkinje cells. The very small marker size of the particles reduces the steric hindrance between antibodies leading to a higher labeling effici...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22043
更新日期:2012-09-01 00:00:00
abstract::Plant diseases are accountable for economic losses in an agricultural country. The manual process of plant diseases diagnosis is a key challenge from last one decade; therefore, researchers in this area introduced automated systems. In this research work, automated system is proposed for citrus fruit diseases recognit...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23320
更新日期:2019-09-01 00:00:00
abstract::Actin, though highly conserved, exhibits a myriad of diverse functions, most of which ultimately depend on its intrinsic ability to rapidly assemble and disassemble filamentous structures. Many organisms synthesize multiple actin isoforms even within the same cell. Tissue-specific expression patterns and tight develop...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991001)47:1<38::AID-JEMT
更新日期:1999-10-01 00:00:00
abstract::Contraction of muscular tissue requires the synchronized shortening of myofibers arrayed in complex geometrical patterns. Imaging such myofiber patterns with diffusion-weighted MRI reveals architectural ensembles that underlie force generation at the organ scale. Restricted proton diffusion is a stochastic process res...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.22777
更新日期:2018-02-01 00:00:00
abstract::Fatty acid and alkyl compositions of 1-alkyl-2,3-diacylglycerols (ADG) in the Harderian gland of the golden hamster were determined. Fatty acids of male ADG consisted of straight chain saturated acids ranging from C12 to C22. Both even- and odd-numbered acids were observed, indicating that acetyl- and propionyl-CoAs w...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19960501)34:1<71::AID-JEMT
更新日期:1996-05-01 00:00:00
abstract::Brain tumor identification using magnetic resonance images (MRI) is an important research domain in the field of medical imaging. Use of computerized techniques helps the doctors for the diagnosis and treatment against brain cancer. In this article, an automated system is developed for tumor extraction and classificat...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23238
更新日期:2019-06-01 00:00:00
abstract::The discovery of new song types in Hawaiian Drosophila species has raised a question of alternative sound production and sound perception mechanisms in Drosophila. For example D. disjuncta males, which produce song with a carrier frequency of 5,000-6,000 Hz, do not vibrate their wings but only tremble them in a very s...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20000715)50:2<161::AID-JEMT8>3.0
更新日期:2000-07-15 00:00:00
abstract::In conventional whole-tooth culture systems, limitation exists regarding maintenance of the vitality of the dental pulp, because this tissue is encased in rigid dentin walls that hinder nutrition supply. We here report a whole tooth-in-jaw-bone culture system of rat mandibular first molars, where transcardiac perfusio...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22072
更新日期:2012-10-01 00:00:00
abstract::Worldwide, breast cancer (BrCa) is currently the leading cause of deaths associated to malignant lesions in adult women. Given that some studies have mentioned that peritumoral adipocytes may contribute to breast carcinogenesis, present work sought to quantitative evaluate the morphometry of these cells in a group of ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22972
更新日期:2018-02-01 00:00:00
abstract::The mechanism of the candidacidal activity of histatins 3 and 5 (Hst) is still a matter of debate. Previous studies have indicated that Hst induce cell permeabilization, generation of reactive oxygen species (ROS) by mitochondria, inhibition of the respiratory chain, and energy-dependent cytotoxic release of ATP. On t...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20161
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND:Splint immobilization of the forearm is often performed in clinical practice. Previous studies investigated the effect of immobilization on bone, cartilage, muscle, and tendon, however, the acute effects on human skin microcirculation and histomorphology remains elusive. METHODS:In 12 healthy, nonsmoking in...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22317
更新日期:2014-01-01 00:00:00
abstract::Vanda tessellata (Roxb.) Hook. ex G. Don. (grey orchid, family Orchidaceae) is an epiphytic orchid of horticultural importance and currently under threat due to overharvesting and habitat destruction. Micropropagation protocols were developed for the production of grey orchid but the survival success of in vitro regen...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23630
更新日期:2020-10-22 00:00:00
abstract::All termite species (Isoptera) are eusocial. These insects live in societies where the division of labor is a predominant characteristic, conditioned by the presence of castes with different morphology, ontogeny, and development. The soldier caste is unique among social insects and it is responsible for colony defense...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20365
更新日期:2006-11-01 00:00:00
abstract::Isoform diversity in striated muscle is largely controlled at the level of transcription. In this review we will concentrate on studies concerning transcriptional regulation of the alkali myosin light chain 1F/3F gene. Uncoupled activity of the MLC1F and 3F promoters, together with complex patterns of transcription in...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000915)50:6<510::AID-JEMT8>3.0
更新日期:2000-09-15 00:00:00
abstract::Polarized microscopy provides unique information on anisotropic samples. In its most complete implementation, namely Mueller microscopy, this technique is well suited for the visualization of fibrillar proteins orientations, with collagen in the first place. However, the intrinsic optical anisotropy of unstained tissu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22530
更新日期:2015-08-01 00:00:00
abstract::In this paper we report the effect of noble gas ions bombardment on the degradation of atomically flat Si(111) surfaces at room and high (400 degrees C-600 degrees C) temperatures. Reflection high energy electron diffraction (RHEED) and reflection electron microscopy (REM) have been used to characterize the topography...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070200406
更新日期:1992-02-15 00:00:00