Abstract:
:Fluorescence correlation spectroscopy (FCS) is a very sensitive technique that can be used, e.g., for the measurement of low concentrations and for the investigation of transport of fluorescent molecules. Fluorescence lifetime imaging (FLIM) provides spatially resolved information about molecular fluorescence lifetimes reflecting the interactions of the molecules with their environment. We have applied simultaneous two-photon FCS and FLIM to probe the behavior of fluorescent molecules diffusing in submicrometer silicon oxide channels. Our measurements reveal differences in fluorescence lifetimes compared to bulk solution that result from the effects of confinement and the presence of interfaces. Confinement also affects diffusional characteristics of fluorophores as reflected in fluorescence autocorrelation functions. These possible consequences of both spatial confinement and the presence of interfaces between media with different refractive indices on the diffusion and fluorescence lifetime of molecules in nanostructures are discussed in general.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Petrásek Z,Krishnan M,Mönch I,Schwille Pdoi
10.1002/jemt.20428subject
Has Abstractpub_date
2007-05-01 00:00:00pages
459-66issue
5eissn
1059-910Xissn
1097-0029journal_volume
70pub_type
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