Skin regeneration stimulation: the role of PCL-platelet gel nanofibrous scaffold.

Abstract:

:Skin is the largest organ of the human body. Thus far, tissue engineering of skin has developed rapidly and has used many types of growth factors and nanofibrous scaffolds. In this study, we differentiated neonate keratinocytes for epithelialization on the polycaprolactone-Platelet gel (PCL-PG) scaffold. Fabricated PCL nanofibers prepared by electrospinning technology and coated by platelet gel. Subsequently, the structure of the scaffold was evaluated by SEM, FTIR-ATR, contact angle and tensile test assays. After seeding the neonate keratinocytes on neat PCL and PCL-PG scaffolds, the epidermal maturation was tested by detecting cytokeratin 10 and loricrin determinants by immunocytochemistry; moreover, keratinocyte genes such as keratin 14, keratin 10, and Involucrin were investigated by real-time PCR. The results of MTT assay indicated an increase in cell viability and cell proliferation of neonate keratinocytes on PCL-PG nanofiber scaffolds compared with PCL. RT-PCR and immunocytochemical analysis showed better cell differentiation on the PCL-PG scaffolds than neat PCL. Furthermore, SEM microscopy images demonstrated that neo-keratinocytes enhance adhesion and proliferation on PCL-PG nanofiber scaffolds. We found that PG increases biocompatibility and wettability of scaffold, cell adhesion, and expression of keratinocyte markers. Overall, this procedure is recommended to be employed in skin tissue engineering and wounds healing.

journal_name

Microsc Res Tech

authors

Ranjbarvan P,Soleimani M,Samadi Kuchaksaraei A,Ai J,Faridi Majidi R,Verdi J

doi

10.1002/jemt.22821

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

495-503

issue

5

eissn

1059-910X

issn

1097-0029

journal_volume

80

pub_type

杂志文章
  • Immune response to autologous and heterologous Helicobacter pylori antigens in humans.

    abstract::Infection of humans with Helicobacter pylori results in the development of chronic gastritis and plays an important role in gastric ulcer pathogenesis. Despite the infiltration of the mucosa with specific immunocompetent cells and production of specific antibodies, the infection usually persists for life. This study w...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1111

    authors: Jakob B,Birkholz S,Schneider T,Duchmann R,Zeitz M,Stallmach A

    更新日期:2001-06-15 00:00:00

  • Induced maturation of frog mast cells by nerve growth factor during ontogenesis.

    abstract::The effect of nerve growth factor (NGF) on ontogenesis of frog mast cells was investigated in vivo by histochemical, morphometric, and ultrastructural analysis. Three groups of tadpoles at various stages of development were used. In the first group, the larvae received i.p. injections of 1 ng NGF/g; the second group r...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10391

    authors: Baccari GC,Raucci F,Di Fiore MM,Monteforte R

    更新日期:2003-12-01 00:00:00

  • Laser-based in situ techniques: novel methods for generating extreme conditions in TEM samples.

    abstract::The dynamic transmission electron microscope (DTEM) is introduced as a novel tool for in situ processing of materials. Examples of various types of dynamic studies outline the advantages and differences of laser-based heating in the DTEM in comparison to conventional (resistive) heating in situ TEM methods. We demonst...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20664

    authors: Taheri ML,Lagrange T,Reed BW,Armstrong MR,Campbell GH,DeHope WJ,Kim JS,King WE,Masiel DJ,Browning ND

    更新日期:2009-03-01 00:00:00

  • Spatial distribution of cytoskeleton intermediate filaments during fetal rat hepatocyte differentiation.

    abstract::The construction of the liver parenchyma throughout fetal development depends on the elaboration of intercellular contacts between epithelial cells and between epithelial and mesenchymal cells. During this time, the spatial distribution of cytokeratins in hepatocytes shows a striking evolution as demonstrated by confo...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971201)39:5<436::AID-JEM

    authors: Vassy J,Irinopoulou T,Beil M,Rigaut JP

    更新日期:1997-12-01 00:00:00

  • Acute lung injury during bacterial or fungal sepsis.

    abstract::We compared physiological and ultrastructural indices of acute lung injury (ALI) during septic shock caused by taxonomically diverse pathogens to distinguish ALI due to endogenous inflammatory mediators vs. microbial exotoxins or other factors. Conscious rats were infected i.v. with gram-negative Escherichia coli (EC,...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070260512

    authors: Lechner AJ,Ryerse JS,Matuschak GM

    更新日期:1993-12-01 00:00:00

  • Light-triggered Janus nanomotor for targeting and photothermal lysis of pathogenic bacteria.

    abstract::The rapid photothermal lysis of Escherichia coli O157:H7 treated with light-triggered Janus nanomotors was visualized by Hilbert differential contrast transmission electron microscopy (HDC-TEM). The extraordinary advantage of this high-resolution microscopic technique was that it revealed the detailed ultrastructure a...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23657

    authors: Loukanov A

    更新日期:2020-11-27 00:00:00

  • Dissociation of memory retrieval and search processes: an event-related fMRI study.

    abstract::A cognitive task can often be subdivided into several subprocesses, which follow a specific temporal order. Here, we report an event-related functional magnetic resonance imaging (fMRI) experiment on memory search, in which the temporal onset of search in primary memory was varied relative to retrieval from secondary ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20001001)51:1<29::AID-JEMT3>3.0.

    authors: Pollmann S,Zysset S,Wiggins CJ,Von Cramon DY

    更新日期:2000-10-01 00:00:00

  • A novel whole tooth-in-jaw-bone culture of rat molars: morphological, immunohistochemical, and laser capture microdissection analysis.

    abstract::In conventional whole-tooth culture systems, limitation exists regarding maintenance of the vitality of the dental pulp, because this tissue is encased in rigid dentin walls that hinder nutrition supply. We here report a whole tooth-in-jaw-bone culture system of rat mandibular first molars, where transcardiac perfusio...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22072

    authors: Chokechanachaisakul U,Kaneko T,Yamanaka Y,Okiji T,Suda H

    更新日期:2012-10-01 00:00:00

  • Evaluation of the effect of food and beverages on enamel and restorative materials by SEM and Fourier transform infrared spectroscopy.

    abstract:OBJECTIVES:To examine different types of restorative materials used in children as well as primary and permanent teeth enamel when affected by erosive foods. MATERIALS AND METHOD:Buttermilk, fruit yoghurt, Coca-cola, fruit juice, Filtek Z-250, Dyract Extra, Fuji II LC, and Fuji IX and tooth enamel were used. Measureme...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22315

    authors: Sari ME,Erturk AG,Koyuturk AE,Bekdemir Y

    更新日期:2014-01-01 00:00:00

  • Electron energy loss spectroscopy for analysis of inhaled ultrafine particles in rat lungs.

    abstract::Epidemiologic studies have associated cardiovascular morbidity and mortality with ambient particulate air pollution. Particles smaller than 100 nm in diameter (ultrafine particles) are present in the urban atmosphere in very high numbers yet at very low mass concentration. Organs beyond the lungs are considered as tar...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20044

    authors: Kapp N,Kreyling W,Schulz H,Im Hof V,Gehr P,Semmler M,Geiser M

    更新日期:2004-04-01 00:00:00

  • Analysis of dynamic cantilever behavior in tapping mode atomic force microscopy.

    abstract::Tapping mode atomic force microscopy (AFM) provides phase images in addition to height and amplitude images. Although the behavior of tapping mode AFM has been investigated using mathematical modeling, comprehensive understanding of the behavior of tapping mode AFM still poses a significant challenge to the AFM commun...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22558

    authors: Deng W,Zhang GM,Murphy MF,Lilley F,Harvey DM,Burton DR

    更新日期:2015-10-01 00:00:00

  • Microscopic and molecular evidence in support of rodent as a reservoir for dissemination of Leishmaniasis.

    abstract::Leishmaniasis is a worldwide public health problem and vector-borne disease. It is caused by a diverse group of protozoan parasites that belong to the genus Leishmania and transmitted to humans through a bite of an infected female sand fly. Leishmaniasis has attained epidemic proportion in Khyber Pakhtunkhwa and raise...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23462

    authors: Khan I,Noor Z,Allauddin Khan S,Ali Khan T,Muhammad N,Ullah Khattak B,Hussain M

    更新日期:2020-07-01 00:00:00

  • The comparison between force volume and peakforce quantitative nanomechanical mode of atomic force microscope in detecting cell's mechanical properties.

    abstract::Atomic force microscope (AFM) has been widely used in the biological field owing to its high sensitivity (subnanonewton), high spatial resolution (nanometer), and adaptability to physiological environments. Nowadays, force volume (FV) and peakforce quantitative nanomechanical (QNM) are two distinct modes of AFM used i...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23351

    authors: Yang Y,Xiao X,Peng Y,Yang C,Wu S,Liu Y,Yue T,Pu H,Liu N,Jiang H

    更新日期:2019-11-01 00:00:00

  • Gap junctions in intestinal smooth muscle and interstitial cells of Cajal.

    abstract::This manuscript reviews gap junctions' roles in control of intestinal motility. Gap junctions (GJs) of small intestine (SmIn) are found between circular muscle (CM) cells, between interstitial cells of Cajal (ICC) of deep muscular plexus (DMP) and between them and adjacent outer circular muscle (OCM). GJs between long...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19991201)47:5<309::AID-JEM

    authors: Daniel EE,Wang YF

    更新日期:1999-12-01 00:00:00

  • Plasticity of retinal ribbon synapses.

    abstract::Ribbon synapses differ from conventional chemical synapses in that they contain, within the cloud of synaptic vesicles (SV's), a specialized synaptic body, most often termed synaptic ribbon (SR). This body assumes various forms. Reconstructions reveal that what appear as rod- or ribbon-like profiles in sections are in...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19961215)35:6<472::AID-JEM

    authors: Vollrath L,Spiwoks-Becker I

    更新日期:1996-12-15 00:00:00

  • GFAP immunoreactivity and transcription in trigeminal and dental tissues of rats and transgenic GFP/GFAP mice.

    abstract::Sensory mechanisms in teeth are not well understood and may involve pulpal-neural interactions. Tooth cells that proliferate in vitro have polyclonal immunoreactivity (IR) for glial fibrillary acidic protein (GFAP), growth-associated protein (GAP-43), and vimentin, plus glial-like ion channels. Here, we analyzed GFAP-...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20130

    authors: Byers MR,Maeda T,Brown AM,Westenbroek RE

    更新日期:2004-12-01 00:00:00

  • Cell-cell interactions in the testis of teleosts and elasmobranchs.

    abstract::In this paper we present the state of knowledge on cell-cell interactions in the testis of two groups of anamniote vertebrates--teleosts and elasmobranchs--which include most fish. In these fish, the structural organization of the testis differs fundamentally from that which characterizes amniotes in which the germina...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070320606

    authors: Loir M,Sourdaine P,Mendis-Handagama SM,Jégou B

    更新日期:1995-12-15 00:00:00

  • Microscopic studies of the effect of laser irradiation parameters of photopolymer plate on the quality of flexographic printing plate produced by the digital method.

    abstract::The process of making of flexographic printing plate by the digital method proceeds in the following stages: back exposure, laser irradiation, main exposure, washing, post exposure, and finishing of the photopolymer plate. Laser irradiation is one of the basic technological stages, in which a negative of the reproduce...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.22418

    authors: Harri L

    更新日期:2016-11-01 00:00:00

  • Unique dentition of lungfish.

    abstract::Lungfish are a group of ancient fish, represented almost continuously in the fossil record from their first appearance in the Devonian to the present time. They have numerous unique characters in the dentition, found only among lungfish, as well as some that are shared with other groups of fish and with higher vertebr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10221

    authors: Kemp A

    更新日期:2002-12-01 00:00:00

  • Surface damage induced by FIB milling and imaging of biological samples is controllable.

    abstract::Focused ion beam (FIB) techniques are among the most important tools for the nanostructuring of surfaces. We used the FIB/SEM (scanning electron microscope) for milling and imaging of digestive gland cells. The aim of our study was to document the interactions of FIB with the surface of the biological sample during FI...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20494

    authors: Drobne D,Milani M,Leser V,Tatti F

    更新日期:2007-10-01 00:00:00

  • Three-dimensional structure of the rat pancreatic duct in normal and inflammated pancreas.

    abstract::We observed the corrosion casts of the Wistar rats' pancreatic ducts with scanning electron microscopy (SEM), and their conventionally fixed pancreatic tissue with SEM and transmission electron microscopy (TEM). These findings revealed the following facts about the three-dimensional structure of pancreatic duct. (1) T...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19970601)37:5/6<543::AID-J

    authors: Ashizawa N,Endoh H,Hidaka K,Watanabe M,Fukumoto S

    更新日期:1997-06-01 00:00:00

  • Plasticity of the superior olivary complex.

    abstract::The superior olivary complex (SOC) is part of the auditory brainstem of the vertebrate brain. Residing ventrally in the rhombencephalon, it receives sensory signals from both cochleae through multisynaptic pathways. Neurons of the SOC are also a target of bilateral descending projections. Ascending and descending effe...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/1097-0029(20001115)51:4<364::AID-JEMT6>3.0

    authors: Illing RB,Kraus KS,Michler SA

    更新日期:2000-11-15 00:00:00

  • Simultaneous detection of protozoa in the tissues of snakes by double in situ hybridization.

    abstract::Different methods have been established for the simultaneous detection of different pathogens in tissue samples, each with certain advantages and disadvantages. Chromogenic in situ hybridization combines specific molecular pathogen detection with microscopic evaluation of pathogen quantity, morphology and distribution...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20546

    authors: Richter B,Fragner K,Weissenböck H

    更新日期:2008-04-01 00:00:00

  • Sexual diversity of the lipid metabolism in the Harderian gland of the golden hamster.

    abstract::Fatty acid and alkyl compositions of 1-alkyl-2,3-diacylglycerols (ADG) in the Harderian gland of the golden hamster were determined. Fatty acids of male ADG consisted of straight chain saturated acids ranging from C12 to C22. Both even- and odd-numbered acids were observed, indicating that acetyl- and propionyl-CoAs w...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19960501)34:1<71::AID-JEMT

    authors: Seyama Y,Otsuka H,Ohashi K,Vivien-Roels B,Pevet P

    更新日期:1996-05-01 00:00:00

  • Confocal imaging of dendritic Ca2+ transients in hippocampal brain slices during simultaneous current- and voltage-clamp recording.

    abstract::Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070290404

    authors: Jaffe DB,Brown TH

    更新日期:1994-11-01 00:00:00

  • Rapid microscope auto-focus method for uneven surfaces based on image fusion.

    abstract::Microscopic imaging of uneven surfaces is difficult because of the limited depth of field. In this study, we developed a rapid auto-focus method for uneven surfaces based on image fusion. The Prewitt operator was used to detect the vertical edges of the images. Then, the focus position was theoretically calculated usi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23328

    authors: Jiang MS,Yang T,Xu XL,Zhang XD,Li F

    更新日期:2019-09-01 00:00:00

  • Assessing the multiscale architecture of muscular tissue with Q-space magnetic resonance imaging: Review.

    abstract::Contraction of muscular tissue requires the synchronized shortening of myofibers arrayed in complex geometrical patterns. Imaging such myofiber patterns with diffusion-weighted MRI reveals architectural ensembles that underlie force generation at the organ scale. Restricted proton diffusion is a stochastic process res...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.22777

    authors: Hoffman MP,Taylor EN,Aninwene GE 2nd,Sadayappan S,Gilbert RJ

    更新日期:2018-02-01 00:00:00

  • Neuropeptides in the crayfish stomatogastric nervous system.

    abstract::Neuropeptides are peptides with profound effects on the nervous system. The function of neuropeptides can be studied in detail in the stomatogastric nervous system (STNS). Neuropeptides are ubiquitously distributed in the STNS and it contains well-studied neural circuits that are strongly modulated by neuropeptides. T...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10269

    authors: Skiebe P

    更新日期:2003-02-15 00:00:00

  • Nondestructive estimation of growth year in ginseng cultivars using the means of mathematical modeling on the basis of allometry.

    abstract::Growth-year authentication has extraordinary significance for plant growth, structure and development research, and has a wide range of applications in value assessment of economic crops. Panax ginseng is the most commonly used medicinal plant in Asian countries. The fix number of growth-year is an important quality e...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22610

    authors: Cheng C,Yuan Q,Zhou H,Huang L

    更新日期:2016-02-01 00:00:00

  • Ultrastructural studies define soluble macromolecular, particulate, and cellular transendothelial cell pathways in venules, lymphatic vessels, and tumor-associated microvessels in man and animals.

    abstract::We present de novo studies and review published efforts from our laboratory, spanning 12 years (from 1988 to 2000), where we have used ultrastructural approaches to study the functional anatomy of the microvasculature in man and animals in health and disease. These efforts have defined a new endothelial cell organelle...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10087

    authors: Feng D,Nagy JA,Dvorak HF,Dvorak AM

    更新日期:2002-06-01 00:00:00