Abstract:
:Gamma interferon (IFN-gamma) plays an important role in experimental Trypanosoma cruzi infections, presumably by controlling the early replication of parasites in host macrophages. In this work, we show that NK cells represent an important cell type responsible for the production of most of the IFN-gamma in the early stage of T. cruzi infection and that the in vivo treatment of mice with anti-NK1.1 monoclonal antibody made resistant animals susceptible to the infection. Through in vitro experiments, we demonstrate that normal splenocytes from euthymic or athymic nude mice cultivated for 48 h with live T. cruzi trypomastigotes produced elevated levels of IFN-gamma. In addition, NK-depleted splenocytes show a drastic reduction of IFN-gamma production in response to live T. cruzi trypomastigotes. We also demonstrated that IFN-gamma production is dependent on a factor secreted by adherent cells. Supernatants of spleen cells from athymic nude mice are able to induce IFN-gamma production by normal splenocytes when cultured with trypomastigotes. The addition of anti-interleukin-10 to these cultures resulted in a marked increase in IFN-gamma production. On the other hand, the absence of NK cells led to an increased secretion of interleukin-10 upon in vitro stimulation with T. cruzi. Taken together, these results suggest that NK cells are the major source of IFN-gamma that could be involved in limiting the replication of T. cruzi in host macrophages during the early acute phase of the infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cardillo F,Voltarelli JC,Reed SG,Silva JSdoi
10.1128/IAI.64.1.128-134.1996subject
Has Abstractpub_date
1996-01-01 00:00:00pages
128-34issue
1eissn
0019-9567issn
1098-5522journal_volume
64pub_type
杂志文章abstract::Lack of efficacious vaccines against Mycobacterium tuberculosis (MTB) infection is a limiting factor in the prevention and control of tuberculosis (TB), the leading cause of death from an infectious agent. Improvement or replacement of the BCG vaccine with one that reliably protects all age-groups is urgent. Concerns ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00585-20
更新日期:2020-12-14 00:00:00
abstract::The biological activity of C-ASWS, an alkali-soluble, water-soluble cell wall antigen isolated from mycelial-phase cells of Coccidioides immitis, was compared with that of a commercial coccidioidin (CDN; Cutter Laboratories); CDN-TS, a toluene-induced lysate of mycelial-phase cells; and spherulin, a spherule-derived e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.751-755.1977
更新日期:1977-03-01 00:00:00
abstract::Peripheral leukocytes from 16 Thai children with dengue hemorrhagic fever were examined to determine the leukocyte composition on the day of presentation and on convalescent days 15 and 30. Mononuclear cells were isolated each time, and the concentrations of T, B, Fc receptor-bearing, and "null" cells were determined....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Porphyromonas gingivalis has been implicated as an etiologic agent of adult periodontitis. We have previously shown that P. gingivalis can degrade the epithelial cell-cell junction complexes, thus suggesting that this bacterium can invade the underlying connective tissues via a paracellular pathway. However, the preci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2512-2518.2002
更新日期:2002-05-01 00:00:00
abstract::Four classes of mutants of type III group B streptococcus were isolated by serial subculture of the wild-type strain in the presence of type III-specific rabbit antiserum. Class I mutants no longer synthesized sialic acid but still elaborated the core antigen. Class II mutants maintained the ability to synthesize sial...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.141-151.1983
更新日期:1983-10-01 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for large numbers of postsurgical nosocomial infections across the United States and worldwide. Propofol anesthesia is widely used in surgery and in intensive care units, and recent evidence indicates that even brief exposure to propofol can substantial...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00097-17
更新日期:2017-06-20 00:00:00
abstract::The antigenicity of Erysipelothrix rhusiopathiae T28 (serotype 2) was investigated. Antigens were solubilized from the cell surface with detergents. By means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting it was shown that the molecular weight of the main antigenic component--a nonprot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.818-822.1986
更新日期:1986-06-01 00:00:00
abstract::The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.757-758.1994
更新日期:1994-02-01 00:00:00
abstract::Increased protection against a virulent challenge with Mycobacterium tuberculosis is induced mainly by a previous immunization with living avirulent mycobacteria, usually Mycobacterium bovis BCG. Only a transient and marginal protection is obtained after immunization with bacterial extracts or dead bacteria. Both livi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.742-750.1993
更新日期:1993-02-01 00:00:00
abstract::Immunocompromised patients are more susceptible to recurrent nontyphoidal Salmonella (NTS) bacteremia. A key manifestation of HIV infection is the loss of CD4 T cells, which are crucial for immunity to Salmonella infection. We characterized the consequences of CD4 T cell depletion in mice where virulent Salmonella est...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00417-20
更新日期:2020-12-15 00:00:00
abstract::Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2416-2419.1987
更新日期:1987-10-01 00:00:00
abstract::The Syrian golden hamster (Mesocricetus auratus) is uniquely susceptible to a variety of intracellular pathogens and is an excellent model for a number of human infectious diseases. The molecular basis for this high level of susceptibility is unknown, and immunological studies related to this model have been limited b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2135-2142.1998
更新日期:1998-05-01 00:00:00
abstract::Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis deliver different Yop (Yersinia outer proteins) effector proteins into mammalian cells by a type III secretion mechanism. Recently, it was shown that Yersinia producing YopT leads to disruption of the actin cytoskeleton of HeLa cells (M. Iriarte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7535-7543.2001
更新日期:2001-12-01 00:00:00
abstract::The NikR protein is a nickel-dependent regulatory protein which is a member of the ribbon-helix-helix family of transcriptional regulators. The gastric pathogen Helicobacter pylori expresses a NikR ortholog, which was previously shown to mediate regulation of metal metabolism and urease expression, but the mechanism g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7252-7258.2005
更新日期:2005-11-01 00:00:00
abstract::The rate of clearance of Vibrio fetus var. venerealis was measured by using in vivo and in vitro methods. The rate of clearance from the circulating blood increased in immune rabbits and reached a plateau 4 days after immunization. Thereafter in vivo clearance did not increase further despite rising agglutinin titers....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.6.815-818.1971
更新日期:1971-06-01 00:00:00
abstract::Pseudomonas aeruginosa is a gram-negative bacillus that is a major cause of morbidity and mortality in immunosuppressed patients, burn patients, and patients with cystic fibrosis. Although immunity to these bacteria has been associated with serum antibody, more recent evidence suggests that T-cell-mediated immunity ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.670-674.1983
更新日期:1983-05-01 00:00:00
abstract::Streptolysin-O (SLO) is a thiol-activated, membrane-damaging protein toxin of Mr 69,000 that is produced by most strains of beta-hemolytic group A streptococci. Native, primarily water-soluble toxin molecules bind to cholesterol-containing target membranes to assemble into supramolecular curved rod structures (25 to 1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.52-60.1985
更新日期:1985-01-01 00:00:00
abstract::Escherichia coli 536 (O6:K15:H31), which was isolated from a case of urinary tract infection, determines high nephropathogenicity in a rat pyelonephritis system as measured by renal bacterial counts 7 days after infection. The loss of S fimbrial adhesin formation (Sfa-) (mannose-resistant hemagglutination [Mrh-] and f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.761-767.1986
更新日期:1986-12-01 00:00:00
abstract::The enteric pathogen Shigella is one of the leading causes of moderate-to-severe diarrhea and death in young children in developing countries. Transformed cell lines and animal models have been widely used to study Shigella pathogenesis. In addition to altered physiology, transformed cell lines are composed of a singl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00740-18
更新日期:2019-03-25 00:00:00
abstract::Toll-like receptors 2 and 4 (TLR2 and TLR4) have been found to transduce signals of peptidoglycan (PGN) and lipopolysaccharide (LPS), respectively, for NF-kappa B activation. However, little is known about the expression and regulation of the TLR2 gene in monocytes/macrophages in response to the two typical bacterial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2788-2796.2001
更新日期:2001-05-01 00:00:00
abstract::Various features of salmonellosis were examined in a burned-mouse model. In this model, which uses an outbred mouse strain, a challenge dose of ca. 100 CFU with any of several strains of Salmonella typhimurium caused a fatal infection. A variety of mutated strains attenuated for virulence in Salmonella-susceptible par...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2842-2846.1989
更新日期:1989-09-01 00:00:00
abstract::Infection with Cryptosporidium parvum, a ubiquitous protozoan parasite of virtually all mammals, can cause mild to severe diarrhea in immunocompetent hosts and life-threatening diarrhea in immunocompromised hosts. Passive immunotherapy of experimentally infected animals and naturally infected humans with hyperimmune b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2962-2965.1990
更新日期:1990-09-01 00:00:00
abstract::The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development of optimal therapeutic strategies for invasive infections. CpsY is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00027-12
更新日期:2012-05-01 00:00:00
abstract::The Lyme disease spirochete, Borrelia burgdorferi, exists in a zoonotic cycle involving an arthropod tick and mammalian host. Dissemination of the organism within and between these hosts depends upon the spirochete's ability to traverse through complex tissues. Additionally, the spirochete outruns the host immune cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01228-12
更新日期:2013-06-01 00:00:00
abstract::Antisera against isolated cell surface preparations (PCSP-As) of Leishmania donovani promastigotes were used to detect extracellular antigens produced during the growth of these organisms in four different growth media. The PCSP-As precipitated two major antigenically identical but electrophoretically distinct compone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.558-567.1982
更新日期:1982-08-01 00:00:00
abstract::Endotoxin-treated mice exhibit a rapid rise in the level of the serum amyloid A (SAA) protein, but this effect is not observed in endotoxin-resistant C3H/HeJ mice. To evaluate the role of lymphoid cells in the production of SAA protein, C3H/HeJ mice were adoptively transfused with endotoxin-sensitive (C3H/HeN) bone ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.1.181-183.1979
更新日期:1979-01-01 00:00:00
abstract::Using polystyrene microspheres coated with heparin or heparan sulfate, it was shown that coated microspheres specifically bound eukaryotic cells and were endocytosed by nonprofessional phagocytic cells. Coated microspheres displayed properties of binding to eukaryotic cells that were similar to those of chlamydiae, an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1080-1085.2000
更新日期:2000-03-01 00:00:00
abstract::A genomic library of meningococcal DNA from a clinical isolate of Neisseria meningitidis was constructed in the expression vector lambda gt11. Outer membrane complex was prepared from the same strain and used to immunize rabbits to raise polyclonal anti-outer membrane complex serum. The amplified library was probed wi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.11.2734-2740.1987
更新日期:1987-11-01 00:00:00
abstract::Photobacterium damselae subsp. damselae causes infections and fatal disease in marine animals and in humans. Highly hemolytic strains produce damselysin (Dly) and plasmid-encoded HlyA (HlyA(pl)). These hemolysins are encoded by plasmid pPHDD1 and contribute to hemolysis and virulence for fish and mice. In this study, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00155-13
更新日期:2013-09-01 00:00:00
abstract::Neutrophil extracellular traps (NETs) are produced by neutrophils as an innate immune defense mechanism to trap and kill microbial pathogens. NETs are comprised of ejected chromatin that forms a lattice structure enmeshed with numerous antimicrobial proteins. In addition to forming the structural backbone of NETs, ext...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00403-18
更新日期:2018-08-22 00:00:00