Abstract：
:In eucaryotes a cell cycle control called a checkpoint ensures that mitosis occurs only after chromosomes are completely replicated and any damage is repaired. The function of this checkpoint in budding yeast requires the RAD9 gene. Here we examine the role of the RAD9 gene in the arrest of the 12 cell division cycle (cdc) mutants, temperature-sensitive lethal mutants that arrest in specific phases of the cell cycle at a restrictive temperature. We found that in four cdc mutants the cdc rad9 cells failed to arrest after a shift to the restrictive temperature, rather they continued cell division and died rapidly, whereas the cdc RAD cells arrested and remained viable. The cell cycle and genetic phenotypes of the 12 cdc RAD mutants indicate the function of the RAD9 checkpoint is phase-specific and signal-specific. First, the four cdc RAD mutants that required RAD9 each arrested in the late S/G2 phase after a shift to the restrictive temperature when DNA replication was complete or nearly complete, and second, each leaves DNA lesions when the CDC gene product is limiting for cell division. Three of the four CDC genes are known to encode DNA replication enzymes. We found that the RAD17 gene is also essential for the function of the RAD9 checkpoint because it is required for phase-specific arrest of the same four cdc mutants. We also show that both X- or UV-irradiated cells require the RAD9 and RAD17 genes for delay in the G2 phase. Together, these results indicate that the RAD9 checkpoint is apparently activated only by DNA lesions and arrests cell division only in the late S/G2 phase.
journal_name
Geneticsjournal_title
Geneticsauthors
Weinert TA,Hartwell LHsubject
Has Abstractpub_date
1993-05-01 00:00:00pages
63-80issue
1eissn
0016-6731issn
1943-2631journal_volume
134pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Recombination of phage lambda attachment sites occurs by sequential exchange of the DNA strands at two specific locations. The first exchange produces a Holliday structure, and the second resolves it to recombinant products. Heterology for base substitution mutations in the region between the two strand exchange point...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:Predictions for the rate of inbreeding (DeltaF) in populations with discrete generations undergoing selection on best linear unbiased prediction (BLUP) of breeding value were developed. Predictions were based on the concept of long-term genetic contributions using a recently established relationship between expected c...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:A spontaneous antimycin A-resistant mutant carrying approximately four extra copies of ADH2 on chromosome XII was isolated from yeast strain 315-1D which lacks a functional copy of ADH1 and thus is antimycin A-sensitive. The additional copies of the normally glucose-repressed ADH2 are expressed during growth on glucos...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:Various measures have been proposed for characterizing the statistical association that arises between alleles at different loci. Hedrick has compared these measures with the standardized measure D' proposed by Lewontin on the grounds that this latter measure is independent of allele frequency. Although D' has the sam...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:The reproductive success of individual fire ant queens (Solenopsis invicta) previously has been shown to be strongly influenced by their genotype at a single enzyme-encoding gene, designated Pgm-3. This paper presents evidence that a second, tightly linked gene, designated Gp-9, is under similarly strong selection in ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:The N-terminal tail of Ndc80 is essential for kinetochore-microtubule binding in human cells but is not required for viability in yeast. We show that the yeast Ndc80 tail is required for timely mitotic progression and accurate chromosome segregation. The tail is essential when cells are limited for Dam1, demonstrating...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.143818
更新日期：2012-10-01 00:00:00
abstract：:Mutations in arg-13 result in slow growth in minimal medium and can suppress mutations in carbamyl phosphate synthase-aspartate carbamyl transferase within the pyrimidine pathway; the exact biochemical function of the gene product is unknown. To understand the role of arg-13 in arginine metabolism, cosmids rescuing gr...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The evidence in support of two types of alternate disjunction in translocation heterozygotes from the German cockroach, Blattella germanica (L.), is reviewed. When unique cytomorphological features occur in the translocation figure, the two types of alternate disjunction are identifiable regardless of the angle of obs...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromo...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.065573
更新日期：2007-03-01 00:00:00
abstract：:Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since approximately 10% of the genes are expressed in the male gametophyte and approximately 9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduce...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.090852
更新日期：2009-04-01 00:00:00
abstract：:A female frog heterozygous at two unlinked loci, specifying electrophoretic forms of mannosephosphate isomerase (MPI) and malate dehydrogenase (MDH) was crossed to male frogs homozygous for different alleles at each locus. In the offspring approximately ten percent proved to be triploid according to nucleolar and chro...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:The effect of genetic hitchhiking on neutral variation is analyzed in subdivided populations with differentiated demes. After fixation of a favorable mutation, the consequences on particular subpopulations can be radically different. In the subpopulation where the mutation first appeared by mutation, variation at link...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.032813
更新日期：2005-01-01 00:00:00
abstract：:The rag4 mutant of Kluyveromyces lactis was previously isolated as a fermentation-deficient mutant, in which transcription of the major glucose transporter gene RAG1 was affected. The wild-type RAG4 was cloned by complementation of the rag4 mutation and found to encode a protein homologous to Snf3 and Rgt2 of Saccharo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:Nonallelic gene conversion has been proposed as a major force in homogenizing the sequences of paralogous genes. In this work, we investigate the extent and characteristics of gene conversion among gene families in nine species of the genus Drosophila. We carried out a genome-wide study of 2855 gene families (includin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.115444
更新日期：2010-05-01 00:00:00
abstract：:We have studied a genome-wide set of single-nucleotide polymorphism (SNP) allele frequency measures for African-American, East Asian, and European-American samples. For this analysis we derived a simple, closed mathematical formulation for the spectrum of expected allele frequencies when the sampled populations have e...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.1.351
更新日期：2004-01-01 00:00:00
abstract：:A large offspring-number diploid biparental multilocus population model of Moran type is our object of study. At each time step, a pair of diploid individuals drawn uniformly at random contributes offspring to the population. The number of offspring can be large relative to the total population size. Similar "heavily ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.144329
更新日期：2013-01-01 00:00:00
abstract：:Sexual isolating mechanisms that act before fertilization are often considered the most important genetic barriers leading to speciation in animals. While recent progress has been made toward understanding the genetic basis of the postzygotic isolating mechanisms of hybrid sterility and inviability, little is known ab...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.103.024364
更新日期：2004-07-01 00:00:00
abstract：:Since the publication of the first comprehensive linkage map for the laboratory mouse, the architecture of recombination as a basic biological process has become amenable to investigation in mammalian model organisms. Here we take advantage of high-density genotyping and the unique pedigree structure of the incipient ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.161653
更新日期：2014-05-01 00:00:00
abstract：:Mitochondria of Neurospora crassa contain a cyanide-resistant alternative respiratory pathway in addition to the cytochrome pathway. The alternative oxidase is present only when electron flow through the cytochrome chain is restricted. Both genomic and cDNA copies for the alternative oxidase gene have been isolated an...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:A scheme for rapidly mapping chromosome rearrangements relative to the physical map of Caenorhabditis elegans is described that is based on hybridization patterns of cloned DNA on meiotic nuclei, as visualized by fluorescent in situ hybridization. From the nearly complete physical map, DNA clones, in yeast artificial ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：:The three-dimensional (3D) organization of chromosomes can influence transcription. However, the frequency and magnitude of these effects remain debated. To determine how changes in chromosome positioning affect transcription across thousands of genes with minimal perturbation, we characterized nuclear organization an...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302978
更新日期：2020-03-01 00:00:00
abstract：:Cells expend a large amount of energy to maintain their DNA sequence. DNA repair pathways, cell cycle checkpoint activation, proofreading polymerases, and chromatin structure are ways in which the cell minimizes changes to the genome. During replication, the DNA-damage tolerance pathway allows the replication forks to...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301600
更新日期：2018-12-01 00:00:00
abstract：:The presence of at least ten mouse LDH-A pseudogenes was demonstrated in the genomic blot analysis, and four different processed pseudogenes have thus far been isolated and characterized. In this report, the nucleotide sequences to two different mouse lactate dehydrogenase-A processed pseudogenes, M11 and M14, were de...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Sequence divergence among the mitochondrial (mt) DNA of nine Lycopersicon and two closely related Solanum species was estimated using the shared fragment method. A portion of each mt genome was highlighted by probing total DNA with a series of plasmid clones containing mt-specific DNA fragments from Lycopersicon penne...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:In this work we study how mutations that change physical properties of cell proteins (stability) affect population survival and growth. We present a model in which the genotype is presented as a set folding free energies of cell proteins. Mutations occur upon replication, so stabilities of some proteins in daughter ce...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.106492
更新日期：2009-10-01 00:00:00
abstract：:Many aspects of plant biology depend on the ubiquitin proteasome system for degradation of regulatory proteins. Ubiquitin E3 ligases confer substrate specificity in this pathway, and SCF-type ligases comprise a major class of E3s. SCF ligases have four subunits: SKP1, CUL1, RBX1, and an F-box protein for substrate rec...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.097675
更新日期：2009-03-01 00:00:00
abstract：:Hybridization between related species results in the formation of an allopolyploid with multiple subgenomes. These subgenomes will each contain complete, yet evolutionarily divergent, sets of genes. Like a diploid hybrid, allopolyploids will have two versions, or homeoalleles, for every gene. Partial functional redund...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301851
更新日期：2019-03-01 00:00:00
abstract：:Biological evolution generates a surprising amount of site-specific variability in protein sequences. Yet, attempts at modeling this process have been only moderately successful, and current models based on protein structural metrics explain, at best, 60% of the observed variation. Surprisingly, simple measures of pro...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300699
更新日期：2018-04-01 00:00:00
abstract：:Population structure parameters commonly used for diploid species are reexamined for the particular case of tetrasomic inheritance (autotetraploid species). Recurrence equations that describe the evolution of identity probabilities for neutral genes in an "island model" of population structure are derived assuming tet...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:Mobile P elements in Drosophila melanogaster cause hybrid dysgenesis if their mobility is not repressed. One type of repression, termed P cytotype, is a complex interaction between chromosomes carrying P elements and cytoplasm and is transmitted through the cytoplasm only of females. Another type of repression is foun...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
