Meiotic origin of triploidy in the frog detected by genetic analysis of enzyme polymorphisms.

Abstract:

:A female frog heterozygous at two unlinked loci, specifying electrophoretic forms of mannosephosphate isomerase (MPI) and malate dehydrogenase (MDH) was crossed to male frogs homozygous for different alleles at each locus. In the offspring approximately ten percent proved to be triploid according to nucleolar and chromosome counts of tail tip cells. Most of these triploids had both maternal alleles at the MDH and MPI loci suggesting that the first meiotic division was repressed. Others seemed to represent a repressed second meiotic division and one animal, a pentaploid, could only have resulted from inhibition of both meiotic divisions of the egg. Densitometer tracings of starch gels stained for 6 phosphogluconate and isocitrate dehydrogenases, expected to be heterozygous in a particular cross, demonstrated that the triploids had twice as much maternal as paternal gene product for each locus, similar to patterns found in triploids produced by nuclear transplantation.

journal_name

Genetics

journal_title

Genetics

authors

Wright DA,Huang CP,Chuoke BD

keywords:

subject

Has Abstract

pub_date

1976-10-01 00:00:00

pages

319-32

issue

2

eissn

0016-6731

issn

1943-2631

journal_volume

84

pub_type

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