Abstract：
:Recombination of phage lambda attachment sites occurs by sequential exchange of the DNA strands at two specific locations. The first exchange produces a Holliday structure, and the second resolves it to recombinant products. Heterology for base substitution mutations in the region between the two strand exchange points (the overlap region) reduces recombination; some mutations inhibit the accumulation of Holliday structures, others inhibit their resolution to recombinant products. To see if heterology also alters the location of the strand exchange points, we determined the segregation pattern of three single and one multiple base pair substitution mutations of the overlap region in crosses with wild type sites. The mutations are known to differ in the severity of their recombination defect and in the stage of strand exchange they affect. The three single mutations behaved similarly: each segregated into both products of recombination, and the two products of a single crossover were frequently nonreciprocal in the overlap region. In contrast, the multiple mutation preferentially segregated into one of the two recombinant products, and the two products of a single crossover appeared to be fully reciprocal. The simplest explanation of the segregation pattern of the single mutations is that strand exchanges occur at the normal locations to produce recombinants with mismatched base pairs that are frequently repaired. The segregation pattern of the multiple mutation is consistent with the view that both strand exchanges usually occur to one side of the mutant site. We suggest that the segregation pattern of a particular mutation is determined by which stage of strand exchange it inhibits and by the severity of the inhibition.
journal_name
Geneticsjournal_title
Geneticsauthors
Bauer CE,Gardner JF,Gumport RI,Weisberg RAsubject
Has Abstractpub_date
1989-08-01 00:00:00pages
727-36issue
4eissn
0016-6731issn
1943-2631journal_volume
122pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The lin-31 gene is required for the proper specification of vulval cell fates in the nematode Caenorhabditis elegans and encodes a member of the winged-helix family of transcription factors. Members of this important family have been identified in many organisms and are known to bind specific DNA targets involved in a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:Adaptive introgression is common in nature and can be driven by selection acting on multiple, linked genes. We explore the effects of polygenic selection on introgression under the infinitesimal model with linkage. This model assumes that the introgressing block has an effectively infinite number of loci, each with an...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301018
更新日期：2018-08-01 00:00:00
abstract：:Loss of heterozygosity (LOH) can be a driving force in the evolution of mitotic/somatic diploid cells, and cellular changes that increase the rate of LOH have been proposed to facilitate this process. In the yeast Saccharomyces cerevisiae, spontaneous LOH occurs by a number of mechanisms including chromosome loss and ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.089250
更新日期：2008-07-01 00:00:00
abstract：:Presynaptic association of homologous chromosomes is a prerequisite to the sequence of events that lead to chiasma formation. This association of homologous chromosomes, as entire units, occurs with probability a, and chiasma formation occurs independently in opposite chromosome arms with probability c. a and c have b...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-04-01 00:00:00
abstract：:The formation of dense core granules (DCGs) requires both the sorting of granule contents from other secretory proteins and a postsorting maturation process. The Tetrahymena thermophila strain SB281 fails to synthesize DCGs, and previous analysis suggested that the defect lay at or near the sorting step. Because this ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:The rag4 mutant of Kluyveromyces lactis was previously isolated as a fermentation-deficient mutant, in which transcription of the major glucose transporter gene RAG1 was affected. The wild-type RAG4 was cloned by complementation of the rag4 mutation and found to encode a protein homologous to Snf3 and Rgt2 of Saccharo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:Different electrophoretic alleles of amylase show associations with particular chromosome 3 inversions in D. pseudoobscura and D. persimilis. Relative adult amylase activities were compared in 37, 37 and 10 strains of D. pseudoobscura, D. persimilis and D. miranda, respectively. Strains carrying the same electrophoret...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-05-01 00:00:00
abstract：:We reported previously that the product of the DNA damage-inducible gene of Saccharomyces cerevisiae, DIN7, belongs to a family of proteins that are involved in DNA repair and replication. The family includes S. cerevisiae proteins Rad2p and its human homolog XPGC, Rad27p and its mammalian homolog FEN-1, and Exonuclea...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The recessive mutation, hop1-1, was isolated by use of a screen designed to detect mutations defective in homologous chromosomal pairing during meiosis in Saccharomyces cerevisiae. Mutants in HOP1 displayed decreased levels of meiotic crossing over and intragenic recombination between markers on homologous chromosomes...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Drosophila melanogaster males deficient for the crystal (cry) locus of the Y chromosome that carry between 15 and 60 copies of the X-linked Stellate (Ste) gene are semisterile, have elevated levels of nondisjunction, produce distorted sperm genotype ratios (meiotic drive), and evince hyperactive transcription of Ste i...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-08-01 00:00:00
abstract：:Thirty-four independent nonviable c-locus mutations (types cal, albino lethal and cas, albino subvital), derived from radiation experiments, were tested for involvement of nearby markers tp, Mod-2, sh-1, and Hbb: 10, 22, and 2 involved, respectively, none of these markers, Mod-2 alone, and Mod-2 plus sh-1. When classi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
abstract：:Three sex-transforming genes (ix, tra-D, and dsx) in D. melanogaster were examined with respect to possible interactions with the NSR strain SR-spirochetes. The SR-spirochetes exerted a lethal effect on XY but not on XX individuals, regardless of whether they were phenotypically males, intersexes, or females. These re...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Inbred strains of mice show differential skin inflammatory reactivity following the topical application of polycyclic hydrocarbons. Strains also differ in the extent to which hepatic aryl hydrocarbon hydroxylase activity is induced by these compounds. Differential inflammatory response and hydroxylase inducibility are...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-08-01 00:00:00
abstract：:DNA double-strand breaks (DSBs) are repaired by homologous recombination (HR) and nonhomologous end-joining (NHEJ). NHEJ in yeast chromosomes has been observed only when HR is blocked, as in rad52 mutants or in the absence of a homologous repair template. We detected yKu70p-dependent imprecise NHEJ at a frequency of a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:Penicillium marneffei is an opportunistic fungal pathogen of humans and the only dimorphic species identified in its genus. At 25 degrees P. marneffei exhibits true filamentous growth, while at 37 degrees P. marneffei undergoes a dimorphic transition to produce uninucleate yeast cells that divide by fission. Members o...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:The hitchhiking model of population genetics predicts that an allele favored by Darwinian selection can replace haplotypes from the same locus previously established at a neutral mutation-drift equilibrium. This process, known as "selective sweep," was studied by comparing molecular variation between the polymorphic I...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Individuals within a species vary in their responses to a wide range of stimuli, partly as a result of differences in their genetic makeup. Relatively little is known about the genetic and neuronal mechanisms contributing to diversity of behavior in natural populations. By studying intraspecies variation in innate avo...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.178491
更新日期：2015-08-01 00:00:00
abstract：:In both pedigree linkage studies and in population-based association studies there has been much interest in the use of modern dense genetic marker data to infer segments of gene identity by descent (ibd) among individuals not known to be related, to increase power and resolution in localizing genes affecting complex ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.137570
更新日期：2012-04-01 00:00:00
abstract：:We present methods for estimating the parameters of inheritance and selection that appear in a quantitative genetic model for the evolution growth trajectories and other "infinite-dimensional" traits that we recently introduced. Two methods for estimating the additive genetic covariance function are developed, a "full...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：:Adherence, an important virulence factor, is mediated by the EPA (Epithelial Adhesin) genes in the opportunistic pathogen Candida glabrata Expression of adhesin-encoding genes requires tight regulation to respond to harsh environmental conditions within the host. The majority of EPA genes are localized in subtelomeric...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301202
更新日期：2018-09-01 00:00:00
abstract：:As an ancient segmental tetraploid, the maize (Zea mays L.) genome contains large numbers of paralogs that are expected to have diverged by a minimum of 10% over time. Nearly identical paralogs (NIPs) are defined as paralogous genes that exhibit > or = 98% identity. Sequence analyses of the "gene space" of the maize i...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.064006
更新日期：2007-01-01 00:00:00
abstract：:The standard approaches to estimation of quantitative genetic parameters and prediction of response to selection on quantitative traits are based on theory derived for populations undergoing random mating. Many studies demonstrate, however, that mating systems in natural populations often involve inbreeding in various...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:The three-dimensional organization of polytene chromosomes within nuclei containing rearranged X chromosomes was examined in male Drosophila melanogaster. Salivary glands of third instar larvae containing either an inverted X chromosome (YSX.YL, In(1)EN/O) or a ring X chromosome (R(1) 2/BSYy+) were fixed, embedded, an...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:In this article we investigate multiplicative effects between genes in relation to heterosis. The extensive literature on heterosis due to multiplicative effects between characters is reviewed, as is earlier work on the genetic description of heterosis. A two-locus diallelic model of arbitrary gene action is used to d...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:Heterogeneous genetic divergence can accumulate across the genome when populations adapt to different habitats while still exchanging alleles. How long does diversification take and how much of the genome is affected? When divergence occurs in parallel from standing genetic variation, how often are the same haplotypes...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300610
更新日期：2018-07-01 00:00:00
abstract：:The white-apricot (wa) allele differs from the wild-type white gene by the presence of the retrovirus-like transposable element copia within the transcription unit. Most RNAs derived from wa have 3' termini within this insertion, and only small amounts of structurally normal RNA are produced. The activity of wa is red...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:A strain of Caenorhabditis elegans was constructed that permits selection of dominant or sex-linked mutations that transform XO animals (normally male) into fertile females, using a feminizing mutation, tra-2(e2046gf), which by itself does not sexually transform XO males. Twenty-three mutations were isolated after che...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Under certain environmental conditions, naturally transforming bacteria are induced to pick up DNA released into the environment by other cells of the same or closely related species and, by homologous recombination, integrate that DNA into their chromosome. The selective pressures responsible for the evolution and ma...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Identifying loci under natural selection from genomic surveys is of great interest in different research areas. Commonly used methods to separate neutral effects from adaptive effects are based on locus-specific population differentiation coefficients to identify outliers. Here we extend such an approach to estimate d...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.092221
更新日期：2008-10-01 00:00:00
abstract：:A mouse homolog of the Drosophila pair-rule gene Odd Oz (Odz4) maps to the critical region of the l7Rn3 locus on mouse chromosome 7. Here we show that Odz4 is an excellent candidate for this allelic series because (1) it spans the entire critical region, (2) the phenotypes correlate with embryonic expression, (3) the ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.034967
更新日期：2005-01-01 00:00:00
