Abstract：
:DNA topoisomerases, enzymes that alter the superhelicity of DNA, have been implicated in such critical cellular functions as transcription, DNA replication, and recombination. In the yeast Saccharomyces cerevisiae, a null mutation in the gene encoding topoisomerase I (TOP1) causes elevated levels of mitotic recombination in the ribosomal DNA (rDNA), but has little effect on growth. We have isolated a missense mutation in TOP1 that causes mitotic hyper-recombination not only in the rDNA, but also at other loci, in addition to causing a number of other unexpected phenotypes. This topoisomerase I mutation (top1-103) causes slow growth, constitutive expression of DNA damage-inducible genes, and inviability in the absence of the double-strand break repair system. Overexpression of top1-103 causes RAD9-dependent cell cycle arrest in G2. We show that the Top1-103 enzyme nicks DNA in vitro, suggesting that it damages DNA directly. We propose that Top1-103 mimics the action of wild-type topoisomerase I in the presence of the anti-tumor drug, camptothecin.
journal_name
Geneticsjournal_title
Geneticsauthors
Levin NA,Bjornsti MA,Fink GRsubject
Has Abstractpub_date
1993-04-01 00:00:00pages
799-814issue
4eissn
0016-6731issn
1943-2631journal_volume
133pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Fifty-two wee mutants that undergo mitosis and cell division at a reduced size compared with wild type have been genetically analyzed. The mutants define two genes, wee1 and cdc2, which control the timing of mitosis. Fifty-one of the mutants map at the wee1 locus, which is unlinked to any known cdc gene. One of the we...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：:The S-M checkpoint ensures that entry into mitosis is dependent on completion of DNA replication. In the fission yeast Schizosaccharomyces pombe, the SM checkpoint mutant cdc2-3w is thought to be defective in receiving the checkpoint signal. To isolate genes that function in the checkpoint pathway, we screened an S. p...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:The distinction between deleterious, neutral, and adaptive mutations is a fundamental problem in the study of molecular evolution. Two significant quantities are the fraction of DNA variation in natural populations that is deleterious and destined to be eliminated and the fraction of fixed differences between species ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:This paper studies the properties of a new class of demographic parameters for age-structured populations and analyzes the effect of natural selection on these parameters. Two new demographic variables are introduced: the entropy of a population and the reproductive potential. The entropy of a population measures the ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:The L-shaped distribution of estimated QTL effects (R(2)) has long been reported. We recently showed that a metabolic mechanism could account for this phenomenon. But other nonexclusive genetic or nongenetic causes may contribute to generate such a distribution. Using analysis and simulations of an additive genetic mo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:A female frog heterozygous at two unlinked loci, specifying electrophoretic forms of mannosephosphate isomerase (MPI) and malate dehydrogenase (MDH) was crossed to male frogs homozygous for different alleles at each locus. In the offspring approximately ten percent proved to be triploid according to nucleolar and chro...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:Despite its importance to agriculture, the genetic basis of heterosis is still not well understood. The main competing hypotheses include dominance, overdominance, and epistasis. NC design III is an experimental design that has been used for estimating the average degree of dominance of quantitative trait loci (QTL) a...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.082867
更新日期：2008-11-01 00:00:00
abstract：:The process designated RIP (repeat-induced point mutation) alters duplicated DNA sequences in the sexual cycle of Neurospora crassa. We tested whether non-Neurospora sequences are susceptible to RIP, explored the basis for the observed immunity to this process of a diverged tandem duplication that probably arose by a ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The genetic requirements for spontaneous and transcription-stimulated mitotic recombination were determined using a recombination system that employs heterochromosomal lys2 substrates that can recombine only by crossover or only by gene conversion. The substrates were fused either to a constitutive low-level promoter ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:Chromatin is organized and compacted in the nucleus through the association of histones and other proteins, which together control genomic activity. Two broad types of chromatin can be distinguished: euchromatin, which is generally transcriptionally active, and heterochromatin, which is repressed. Here we examine the ...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.117.300386
更新日期：2018-02-01 00:00:00
abstract：:We have used a novel method to activate the DNA damage S-phase checkpoint response in Saccharomyces cerevisiae to slow lagging-strand DNA replication by exposing cells expressing a drug-sensitive DNA polymerase δ (L612M-DNA pol δ) to the inhibitory drug phosphonoacetic acid (PAA). PAA-treated pol3-L612M cells arrest a...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.131938
更新日期：2011-12-01 00:00:00
abstract：:Expectations for the time scale and structure of allelic genealogies in finite populations are formed under three models of sporophytic self-incompatibility. The models differ in the dominance interactions among the alleles that determine the self-incompatibility phenotype: In the SSIcod model, alleles act codominantl...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:This report examines five different naturally occurring alcohol dehydrogenase-1 alleles via the recombinational behavior of Adh1(-) mutants induced within them. Twenty-two biochemically characterized Adh1(-) mutants have been assessed for ability to recombine intragenically, using data generated by specifically staini...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:Strong deviation of allele frequencies from Mendelian inheritance favoring Mus spretus-derived alleles has been described previously for X-linked loci in four mouse interspecific crosses. We reanalyzed data for three of these crosses focusing on the location of the gene(s) controlling deviation on the X chromosome and...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:The change in frequencies of D. melanogaster and D. simulans in competition experiments was used to measure the effect of radiation on the fitness of a population. A dose of 250 or 500 rads given to the males of highly inbred lines of D. simulans at the beginning of competition and every three weeks thereafter increas...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-11-01 00:00:00
abstract：:In order to examine the operation of diversifying selection as the maintenance mechanism of excessive additive genetic variance for viability in southern populations in comparison with northern populations of Drosophila melanogaster, two sets of experiments were conducted using second chromosomes extracted from the Og...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Phenoxazinone synthetase, which catalyzes the condensation of 3-hydroxykynurenine to xanthommatin, the brown eye pigment of Drosophila, is shown to exist in association with a particle which resembles the cytologically defined Type I pigment granule. Several classical eye color mutants (v, cn, st, ltd, cd, w), includi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-01-01 00:00:00
abstract：:In a previous study, a mitochondrial mutant expressing a specific enzymatic deficiency in co-enzyme QH2-cytochrome c reductase was described (TZAGO-LOFF, FOURY and AKAI 1976). Analysis of the mitochondrially translated proteins revealed the absence in the mutant of the mitochondrial product corresponding to cytochrome...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:An elevated mutation rate can provide cells with a source of mutations to adapt to changing environments. We identified a negative epistatic interaction involving naturally occurring variants in the MLH1 and PMS1 mismatch repair (MMR) genes of Saccharomyces cerevisiae We hypothesized that this MMR incompatibility, cre...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.199513
更新日期：2017-04-01 00:00:00
abstract：:A plausible explanation for many MHC-linked diseases is lacking. Sequencing of the MHC class I region (coding units or full contigs) in several human and nonhuman primate haplotypes allowed an analysis of single nucleotide variations (SNV) across this entire segment. This diversity was not evenly distributed. It was r...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.057034
更新日期：2006-07-01 00:00:00
abstract：:The evolution of domesticated maize from its wild ancestor teosinte is a dramatic example of the effect of human selection on agricultural crops. Maize has one dominant axis of growth, whereas teosinte is highly branched. The axillary branches in maize are short and feminized whereas the axillary branches of teosinte ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:Previously we have shown that the nucleolus organizer region (NOR) of Neurospora crassa changes size frequently during the premeiotic portion of the sexual phase. Here, we have investigated whether these changes in size originate only in specific regions of the NOR, or are distributed throughout the NOR. In two specia...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:In order to learn more about other proteins that may be involved in repression of HML and HMR in Saccharomyces cerevisiae, extragenic suppressor mutations were identified that could restore repression in cells defective in SIR4, a gene required for function of the silencer elements flanking HML and HMR. These suppress...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:Nonrandom associations between alleles at different loci can be tested for using Fisher's exact test. Extensive simulations show that there is a substantial probability of obtaining significant nonrandom associations between closely or completely linked polymorphic neutral loci in a population of constant size at equi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:The polarized partitioning of proteins in cells underlies asymmetric cell division, which is an important driver of development and cellular diversity. The budding yeast Saccharomyces cerevisiae divides asymmetrically, like many other cells, to generate two distinct progeny cells. A well-known example of an asymmetric...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303439
更新日期：2020-11-01 00:00:00
abstract：:The behaviour of two "meiotic drive" systems, Segregation-Distorter (SD) and the sex chromosome sc(4)sc(8) has been examined in the same meiocyte. It has been found that the two systems interact in a specific way. When the distorting effects of SD and sc(4)sc(8) are against each other, there is no detectable interacti...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-09-01 00:00:00
abstract：:Electrophoretic comparisons of 40S and 55S ribosomal subunit proteins from 18 strains of Drosophila melanogaster revealed the virtual absence of allelic variation. More detailed two-dimensional studies on the large subunit proteins in 6 of the strains demonstrated additional complexity but still no interstrain variati...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-12-01 00:00:00
abstract：:We describe here a new variant of transposon Tn10 especially adapted for transposon analysis of cloned yeast genes; it can equally well be used for analysis of prokaryotic genes. We have applied this element to analysis of the LEU2, RAD50, and CDC48 genes of Saccharomyces cerevisiae. This transposon, nicknamed mini-Tn...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Heterogeneous genetic divergence can accumulate across the genome when populations adapt to different habitats while still exchanging alleles. How long does diversification take and how much of the genome is affected? When divergence occurs in parallel from standing genetic variation, how often are the same haplotypes...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300610
更新日期：2018-07-01 00:00:00
abstract：:The Saccharomyces cerevisiae nuclear gene RPM2 encodes a component of the mitochondrial tRNA-processing enzyme RNase P. Cells grown on fermentable carbon sources do not require mitochondrial tRNA processing activity, but still require RPM2, indicating an additional function for the Rpm2 protein. RPM2-null cells arrest...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
