Abstract：
:The Saccharomyces cerevisiae nuclear gene RPM2 encodes a component of the mitochondrial tRNA-processing enzyme RNase P. Cells grown on fermentable carbon sources do not require mitochondrial tRNA processing activity, but still require RPM2, indicating an additional function for the Rpm2 protein. RPM2-null cells arrest after 25 generations on fermentable media. Spontaneous mutations that suppress arrest occur with a frequency of approximately 9 x 10(-6). The resultant mutants do not grow on nonfermentable carbon sources. We identified two loci responsible for this suppression, which encode proteins that influence proteasome function or assembly. PRE4 is an essential gene encoding the beta-7 subunit of the 20S proteasome core. A Val-to-Phe substitution within a highly conserved region of Pre4p that disrupts proteasome function suppresses the growth arrest of RPM2-null cells on fermentable media. The other locus, UMP1, encodes a chaperone involved in 20S proteasome assembly. A nonsense mutation in UMP1 also disrupts proteasome function and suppresses Deltarpm2 growth arrest. In an RPM2 wild-type background, pre4-2 and ump1-2 strains fail to grow at restrictive temperatures on nonfermentable carbon sources. These data link proteasome activity with Rpm2p and mitochondrial function.
journal_name
Geneticsjournal_title
Geneticsauthors
Lutz MS,Ellis SR,Martin NCkeywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
1013-23issue
3eissn
0016-6731issn
1943-2631journal_volume
154pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The K1 killer plasmid, [KIL-k1], of Saccharomyces cerevisiae is a 1.25 x 10(6) dalton linear double-stranded RNA plasmid coding for a protein toxin and immunity to that toxin. The [KIL-sd1] plasmid is a replication-defective mutant of [KIL-k1] that depends on one of the recessive chromosomal superkiller (ski-) mutatio...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:In Saccharomyces cerevisiae, 59 of the 78 ribosomal proteins are encoded by duplicated genes that, in most cases, encode identical or very similar protein products. However, different sets of ribosomal protein genes have been identified in screens for various phenotypes, including life span, budding pattern, and drug ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.136549
更新日期：2012-05-01 00:00:00
abstract：:As the dynamic nature of progenitor genomes accompanies the speciation by interspecific hybridization, the extraction of the constituent subgenome(s) from a natural allopolyploid species of long history and then restitution of the progenitor(s) provides the unique opportunity to study the genome evolution and interpla...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.190967
更新日期：2016-11-01 00:00:00
abstract：:LARK is an essential Drosophila RNA-binding protein of the RNA recognition motif (RRM) class that functions during embryonic development and for the circadian regulation of adult eclosion. LARK protein contains three consensus RNA-binding domains: two RRM domains and a retroviral-type zinc finger (RTZF). To show that ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Determining the patterns of DNA sequence variation in the human genome is a useful first step toward identifying the genetic basis of a common disease. A haplotype map (HapMap), aimed at describing these variation patterns across the entire genome, has been recently developed by the International HapMap Consortium. In...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.029603
更新日期：2004-09-01 00:00:00
abstract：:We describe here a family of P elements that we refer to as type I repressors. These elements are identified by their repressor functions and their lack of any deletion within the first two-thirds of the canonical P sequence. Elements belonging to this repressor class were isolated from P strains and were made in vitr...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance (R) genes (Xa4, xa5, xa13, and Xa21) and 12 Xoo races. Our results indicate that these two resistan...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:In the genome of Drosophila melanogaster there is a single locus, Triplo-lethal (Tpl), that causes lethality when present in either one or three copies in an otherwise diploid animal. Previous attempts to mutagenize Tpl produced alleles that were viable over a chromosome bearing a duplication of Tpl, but were not leth...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Results of immunogenetic, immunochemical and physicochemical investigations on two serum allotypes of swine are reported. The allotypes, designated Lpr1 and Lpr2, have been identified by specific alloprecipitins in agar gel. Genetic studies indicate that the allotypes are specified by two codominant autosomal allelic ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:Stress caused by the increased salinity of irrigated fields impairs plant growth and is one of the major constraints that limits crop productivity in many important agricultural areas. As a contribution to solving such agronomic problems, we have carried out a large-scale screening for Arabidopsis thaliana mutants ind...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Heterozygous inactivation of either human TSC1 or TSC2 causes tuberous sclerosis (TSC), in which development of benign tumors, hamartomas, occurs via a two-hit mechanism. In this study, fission yeast genes homologous to TSC1 and TSC2 were identified, and their protein products were shown to physically interact like th...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：:In Saccharomyces cerevisiae, expression of the transcriptional activator GCN4 increases at the translational level in response to starvation for an amino acid. The products of multiple GCD genes are required for efficient repression of GCN4 mRNA translation under nonstarvation conditions. The majority of the known GCD...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects; however, the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes and pathways importa...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300527
更新日期：2018-02-01 00:00:00
abstract：:DnaB is the helicase associated with the DNA polymerase III replication fork in Escherichia coli. Previously we observed that the dnaB107(ts) mutation, at its permissive temperature, greatly stimulated deletion events at chromosomal tandem repeats. This stimulation required recA, which suggests a recombinational mecha...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:About 450 black rats (Rattus rattus) were collected from 25 localities in Asia and Oceania. Their serum transferrins were analyzed by a newly developed thin layer acrylamide gel electrophoresis accompanied with acrinol pretreatment, exhibiting 12 transferrin bands. Generally, Asian type rats (2N=42) had fast-moving tr...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1975-02-01 00:00:00
abstract：:Dimethylnitrosamine and diethylnitrosamine, two potent carcinogens, are nonmutagenic when tested directly in microorganisms. Likewise 1-naphthylamine and 2-naphthylamine are also nonmutagenic but the N-hydroxy derivatives are mutagenic in microorganisms. Apparently these compounds require metabolism to breakdown produ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-07-01 00:00:00
abstract：:The mutation process at microsatellite loci typically occurs at high rates and with stepwise changes in allele sizes, features that may introduce bias when using classical measures of population differentiation based on allele identity (e.g., F(ST), Nei's Ds genetic distance). Allele size-based measures of differentia...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:A novel method of RNA secondary structure prediction based on a comparison of nucleotide sequences is described. This method correctly predicts nearly all evolutionarily conserved secondary structures of five different RNAs: tRNA, 5S rRNA, bacterial ribonuclease P (RNase P) RNA, eukaryotic small subunit rRNA, and the ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:We propose a novel approximate-likelihood method to fit demographic models to human genomewide single-nucleotide polymorphism (SNP) data. We divide the genome into windows of constant genetic map width and then tabulate the number of distinct haplotypes and the frequency of the most common haplotype for each window. W...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.099275
更新日期：2009-05-01 00:00:00
abstract：:The human pathogen Candida albicans is considered an obligate commensal of animals, yet it is occasionally isolated from trees, shrubs, and grass. We generated genome sequence data for three strains of C. albicans that we isolated from oak trees in an ancient wood pasture, and compared these to the genomes of over 200...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301482
更新日期：2019-01-01 00:00:00
abstract：:The rate of microsatellite mutation is dependent upon both the allele length and the repeat motif, but the exact nature of this relationship is still unknown. We analyzed data on the inheritance of human Y-chromosomal microsatellites in father-son duos, taken from 24 published reports and comprising 15,285 directly ob...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.132308
更新日期：2011-12-01 00:00:00
abstract：:Restriction fragment length polymorphisms in mitochondrial DNA (mtDNA) were used to reconstruct evolutionary relationships of conspecific populations in four species of freshwater fish-Amia calva, Lepomis punctatus, L. gulosus, and L. microlophus. A suite of 14-17 endonucleases was employed to assay mtDNAs from 305 sp...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:We describe allelic variation at 28 gene loci in natural populations of D. willistoni. Seventy samples were studied from localities extending from Mexico and Florida, through Central America, the West Indies, and tropical South America, down to South Brazil. At least several hundred, and often several thousand, genome...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-01-01 00:00:00
abstract：:The Saccharomyces cerevisiae PHO85 gene encodes a nonessential cyclin-dependent kinase that associates with 10 cyclin subunits. To survey the functions provided by Pho85, we identified mutants that require PHO85 for viability. We identified mutations that define seven Pho Eighty-Five Requiring or Efr loci, six of whic...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Genes with unstable transcripts often encode proteins that play important regulatory roles. ATL2 is a member of a multigene family coding highly related RING-H2 zinc-finger proteins that may function as E3 ubiquitin ligases. ATL2 mRNA accumulation occurs rapidly and transiently after incubation with elicitors of patho...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.028043
更新日期：2004-06-01 00:00:00
abstract：:The Yanomama Indians are a South American tribe distributed over an irregular area approximately 200 X 300 miles. The gene frequencies observed at 12 loci in 47 villages within this area have been analyzed for the occurrence of clines. Apparently significant clines are observed for alleles of the Rh, MNSs, Kidd, Gm, I...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:The point mutation rate of phage PhiX174 was determined using the fluctuation test. After identifying the genetic changes associated with the selected phenotype, we obtained an estimate of 1.0x10(-6) substitutions per base per round of copying, which is consistent with Drake's rule (0.003 mutations per genome per roun...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.106005
更新日期：2009-10-01 00:00:00
abstract：:Natural selection leaves its footprints on protein-coding sequences by modulating their silent and replacement evolutionary rates. In highly expressed genes in invertebrates, these footprints are seen in the higher codon usage bias and lower synonymous divergence. In mammals, the highly expressed genes have a shorter ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.028944
更新日期：2004-09-01 00:00:00
abstract：:We demonstrate the involvement of suppressor of meiotic silencing-2 (sms-2(+)), a Neurospora gene coding for an Argonaute-like protein, in meiotic silencing and normal sexual development. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:Genome-wide association mapping is a popular method for using natural variation within a species to generate a genotype-phenotype map. Statistical association between an allele at a locus and the trait in question is used as evidence that variation at the locus is responsible for variation of the trait. Indirect assoc...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.121665
更新日期：2010-11-01 00:00:00
