The Triplo-lethal locus of Drosophila: reexamination of mutants and discovery of a second-site suppressor.

Abstract:

:In the genome of Drosophila melanogaster there is a single locus, Triplo-lethal (Tpl), that causes lethality when present in either one or three copies in an otherwise diploid animal. Previous attempts to mutagenize Tpl produced alleles that were viable over a chromosome bearing a duplication of Tpl, but were not lethal in combination with a wild-type chromosome, as deficiencies for Tpl are. These mutations were interpreted as hypomorphic alleles of Tpl. In this work, we show that these alleles are not mutations at Tpl; rather, they are dominant mutations in a tightly linked, but cytologically distant, locus that we have named Suppressor-of-Tpl (Sul(Tpl)). Su(Tpl) mutations suppress the lethality associated with three copies of the Triplo-lethal locus and are recessive lethal. We have mapped Su(Tpl) to the approximate map position 3-46.5, within the cytological region 76B-76D.

journal_name

Genetics

journal_title

Genetics

authors

Dorer DR,Ezekiel DH,Christensen AC

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

1037-42

issue

3

eissn

0016-6731

issn

1943-2631

journal_volume

141

pub_type

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