Abstract:
:Heterozygous inactivation of either human TSC1 or TSC2 causes tuberous sclerosis (TSC), in which development of benign tumors, hamartomas, occurs via a two-hit mechanism. In this study, fission yeast genes homologous to TSC1 and TSC2 were identified, and their protein products were shown to physically interact like the human gene products. Strains lacking tsc1(+) or tsc2(+) were defective in uptake of nutrients from the environment. An amino acid permease, which is normally positioned on the plasma membrane, aggregated in the cytoplasm or was confined in vacuole-like structures in Deltatsc1 and Deltatsc2 strains. Deletion of tsc1(+) or tsc2(+) also caused a defect in conjugation. When a limited number of the cells were mixed, they conjugated poorly. The conjugation efficiency was improved by increased cell density. Deltatsc1 cells were not responsive to a mating pheromone, P-factor, suggesting that Tsc1 has an important role in the signal cascade for conjugation. These results indicate that the fission yeast Tsc1-Tsc2 complex plays a role in the regulation of protein trafficking and suggest a similar function for the human proteins. We also show that fission yeast Int6 is involved in a similar process, but functions in an independent genetic pathway.
journal_name
Geneticsjournal_title
Geneticsauthors
Matsumoto S,Bandyopadhyay A,Kwiatkowski DJ,Maitra U,Matsumoto Tkeywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
1053-63issue
3eissn
0016-6731issn
1943-2631journal_volume
161pub_type
杂志文章相关文献
GENETICS文献大全abstract::Disease resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) in the cultivated tomato, Lycopersicon esculentum, and the closely related L. pimpinellifolium is triggered by the physical interaction between plant disease resistance protein, Pto, and the pathogen avirulence protein, AvrPto. To inves...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.039339
更新日期:2005-09-01 00:00:00
abstract::MiRNAs bear an increasing number of functions throughout development and in the aging adult. Here we address their role in establishing sexually dimorphic traits and sexual identity in male and female Drosophila. Our survey of miRNA populations in each sex identifies sets of miRNAs differentially expressed in male and...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.169268
更新日期:2014-10-01 00:00:00
abstract::The region containing subdivisions 93C, 93D and 93E on chromosome 3 of Drosophila melanogaster has been screened for visible and lethal mutations. Treatment with three mutagens, gamma irradiation, ethyl methanesulfonate and diepoxybutane, has produced mutations that fall into 20 complementation groups, including the p...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::Site-specific recombinases are widely used tools for analysis of genetics, development, and cell biology, and many schemes have been devised to alter gene expression by recombinase-mediated DNA rearrangements. Because the FRT and lox target sites for the commonly used FLP and Cre recombinases are asymmetrical, and mus...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303394
更新日期:2020-08-01 00:00:00
abstract::Biology is characterized by complex interactions between phenotypes, such as recursive and simultaneous relationships between substrates and enzymes in biochemical systems. Structural equation models (SEMs) can be used to study such relationships in multivariate analyses, e.g., with multiple traits in a quantitative g...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.112979
更新日期:2010-06-01 00:00:00
abstract::The distribution of the number of HLA haplotypes shared by sibs affected with the same HLA-linked disease can be used to obtain information on the genetics of the disease. Since the inception of the use of sib-pair methods for the analysis of the HLA-associated diseases, the question has been raised of how to include ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::Two replications of a complete diallel cross experiment were performed among four partially inbred lines of mice. These inbred lines originated from a random-bred ICR strain and were produced by 12 generations of full sibbing (F congruent to 92%). Individual body weight was recorded for each animal at 12, 21, 42 and 5...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-10-01 00:00:00
abstract::The mutation process at microsatellite loci typically occurs at high rates and with stepwise changes in allele sizes, features that may introduce bias when using classical measures of population differentiation based on allele identity (e.g., F(ST), Nei's Ds genetic distance). Allele size-based measures of differentia...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::We have isolated 15 spontaneous mutants resistant to one or several antibiotics like chloramphenicol, erythromycin and spiramycin. We have shown by several criteria that all of them result from mutations localized in the mitochondrial DNA. The mutations have been mapped by allelism tests and by two- and three-factor c...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-12-01 00:00:00
abstract::The 73AD salivary chromosome region of Drosophila melanogaster was subjected to mutational analysis in order to (1) generate a collection of chromosome breakpoints that would allow a correlation between the genetic, cytological and molecular maps of the region and (2) define the number and gross organization of comple...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Mutations in the genealogy of the sequences in a random sample from a population can be classified as external and internal. External mutations are mutations that occurred in the external branches and internal mutations are mutations that occurred in the internal branches of the genealogy. Under the assumption of sele...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::We have examined the stability of duplicated DNA sequences in the sexual phase of the life cycle of the basidiomycete fungus, Coprinus cinereus. We observed premeiotic de novo methylation in haploid nuclei containing either a triplication, a tandem duplication, or an ectopic duplication. Methylation changes were not o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::Transcription of the Saccharomyces MAL structural genes is induced 40-fold by maltose and requires the MAL-activator and maltose permease. To identify additional players involved in regulating MAL gene expression, we carried out a genetic selection for MAL constitutive mutants. Strain CMY4000 containing MAL1 and integ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.029611
更新日期:2004-10-01 00:00:00
abstract::Infantile neuronal ceroid lipofuscinosis (INCL) is a pediatric neurodegenerative disease caused by mutations in the human CLN1 gene. CLN1 encodes palmitoyl-protein thioesterase 1 (PPT1), suggesting an important role for the regulation of palmitoylation in normal neuronal function. To further elucidate Ppt1 function, w...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.067983
更新日期:2007-05-01 00:00:00
abstract::We present a two-part system for conditional FLP-out of FRT-flanked sequences in Caenorhabditis elegans to control gene activity in a spatially and/or temporally regulated manner. Using reporters, we assess the system for efficacy and demonstrate its use as a cell lineage marking tool. In addition, we construct and te...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.090274
更新日期:2008-09-01 00:00:00
abstract::The long-term population genetics of multigene families is influenced by several biased and unbiased mechanisms of nonreciprocal exchanges (gene conversion, unequal exchanges, transposition) between member genes, often distributed on several chromosomes. These mechanisms cause fluctuations in the copy number of varian...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::Previous work showed that C. elegans gon-14 is required for gonadogenesis. Here we report that gon-14 encodes a protein with similarity to LIN-15B, a class B synMuv protein. An extensive region of GON-14 contains blocks of sequence similarity to transposases of the hAT superfamily, but key residues are not conserved, ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.048751
更新日期:2006-02-01 00:00:00
abstract::Despite its importance to agriculture, the genetic basis of heterosis is still not well understood. The main competing hypotheses include dominance, overdominance, and epistasis. NC design III is an experimental design that has been used for estimating the average degree of dominance of quantitative trait loci (QTL) a...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.082867
更新日期:2008-11-01 00:00:00
abstract::Genetic analyses of nine traits associated with stem water-soluble carbohydrate (SWSC) accumulation and remobilization at grain-filling period under drought stress (DS) and well-watered (WW) conditions were undertaken using doubled haploid lines (DHLs) derived from two Chinese common wheat cultivars. Some significantl...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.068361
更新日期:2007-05-01 00:00:00
abstract::The rdgB mutants depend on recombinational repair of double-strand breaks. To assess other consequences of rdgB inactivation in Escherichia coli, we isolated RdgB-dependent mutants. All transposon inserts making cells dependent on RdgB inactivate genes of double-strand break repair, indicating that chromosomal fragmen...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.051144
更新日期:2006-02-01 00:00:00
abstract::We introduce an approach to revealing the likelihood of different population histories that utilizes an explicit model of sequence evolution for the DNA segment under study. Based on a phylogenetic tree reconstruction method we show that a Tamura-Nei model with heterogeneous mutation rates is a fair description of the...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::Bonus, a Drosophila TIF1 homolog, is a nuclear receptor cofactor required for viability, molting, and numerous morphological events. Here we establish a role for Bonus in the modulation of chromatin structure. We show that weak loss-of-function alleles of bonus have a more deleterious effect on males than on females. ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.037085
更新日期:2005-02-01 00:00:00
abstract::A lethal locus (l(2)br7;35B6-10), near Adh on chromosome arm 2L of D. melanogaster, is identified with Plunkett's dominant suppressor of Hairless (H). Of eight new alleles, seven act as dominant suppressors of H, the eighth is a dominant enhancer of H. One of the suppressor alleles is both a leaky lethal and a weak s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::Vegetative incompatibility in fungi has long been known to reduce the transmission of viruses between individuals, but the barrier to transmission is incomplete. In replicated laboratory assays, we showed conclusively that the transmission of viruses between individuals of the chestnut blight fungus Cryphonectria para...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::The genetic etiology of many complex diseases is highly heterogeneous. A complex disease can be caused by multiple mutations within the same gene or mutations in multiple genes at various genomic loci. Although these disease-susceptibility mutations can be collectively common in the population, they are often individu...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301266
更新日期:2018-10-01 00:00:00
abstract::The supramolecular striatin-interacting phosphatases and kinases (STRIPAK) complex is conserved from yeast to human, and regulates a variety of key biological processes. In animals, this complex consists of the scaffold protein striatin, the protein phosphatase 2A, and kinases, such as germinal center kinase (GCK) III...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301261
更新日期:2018-09-01 00:00:00
abstract::TE146 is a giant transposon of Drosophila melanogaster. It carries two copies of the white and roughest genes, normally found on the X chromosome. The structure of this transposon has been studied at the molecular level. TE146 may transpose to new chromosome positions, excise and be lost from the genome or undergo int...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::The third hypervariable (V3) region of the HIV-1 gp120 protein is responsible for many aspects of viral infectivity. The tertiary structure of the V3 loop seems to influence the coreceptor usage of the virus, which is an important determinant of HIV pathogenesis. Hence, the information about preferred conformations of...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.051508
更新日期:2006-03-01 00:00:00
abstract::Evolution through natural selection suggests unnecessary genes are lost. We observed that the yeast Candida glabrata lost the gene encoding a phosphate-repressible acid phosphatase (PHO5) present in many yeasts including Saccharomyces cerevisiae. However, C. glabrata still had phosphate starvation-inducible phosphatas...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.120824
更新日期:2010-11-01 00:00:00
abstract::We constructed a genetic linkage map of Gibberella zeae (Fusarium graminearum) by crossing complementary nitrate-nonutilizing (nit) mutants of G. zeae strains R-5470 (from Japan) and Z-3639 (from Kansas). We selected 99 nitrate-utilizing (recombinant) progeny and analyzed them for amplified fragment length polymorphis...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00