Abstract：
:We present a two-part system for conditional FLP-out of FRT-flanked sequences in Caenorhabditis elegans to control gene activity in a spatially and/or temporally regulated manner. Using reporters, we assess the system for efficacy and demonstrate its use as a cell lineage marking tool. In addition, we construct and test a dominant-negative form of hlh-12, a gene that encodes a basic helix-loop-helix (bHLH) transcription factor required for proper distal tip cell (DTC) migration. We show that this allele can be conditionally expressed from a heat-inducible FLP recombinase and can interfere with DTC migration. Using the same DTC assay, we conditionally express an hlh-12 RNAi-hairpin and induce the DTC migration defect. Finally, we introduce a set of traditional and Gateway-compatible vectors to facilitate construction of plasmids for this technology using any promoter, reporter, and gene/hairpin of interest.
journal_name
Geneticsjournal_title
Geneticsauthors
Voutev R,Hubbard EJdoi
10.1534/genetics.108.090274subject
Has Abstractpub_date
2008-09-01 00:00:00pages
103-19issue
1eissn
0016-6731issn
1943-2631pii
genetics.108.090274journal_volume
180pub_type
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