Abstract:
:Treatment of Rickettsia prowazekii-infected L929 cells with gamma interferon (IFN-gamma) immediately after infection altered the lipid metabolism of the host cells as determined by measurement of phospholipid hydrolysis and oleic acid incorporation into phospholipids and neutral lipids. At 48 h postinfection, there was increased phospholipid hydrolysis in infected cultures relative to mock-infected cultures and a further increase in radiolabeled phospholipid hydrolysis in IFN-gamma-treated infected cultures. Oleic acid, the radiolabeled product of hydrolysis, was found in both the free fatty acid and neutral lipid fractions. None of the mock-infected cultures demonstrated increased hydrolysis of their radiolabeled phospholipids in response to treatment with IFN-gamma. Most of the radiolabeled oleic acid incorporated into cultures in the interval between 24 and 48 h after infection and IFN-gamma treatment was present in the phospholipid fraction. However, the neutral lipid fraction from the infected cultures that had been IFN-gamma treated was labeled to a greater extent than that from the untreated cultures. Thin-layer chromatographic analysis of the neutral lipid fractions from both the hydrolysis and incorporation experiments demonstrated that most of the radiolabel was in triglycerides. The infected cultures at 30 h were intact as assessed by the exclusion of trypan blue, but at 48 h postinfection in the IFN-gamma-treated infected cultures more than half of the cells were unable to exclude trypan blue. In no case did the mock-infected cells show substantial damage as a result of IFN-gamma treatment.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Winkler HH,Day L,Daugherty R,Turco Jdoi
10.1128/IAI.61.8.3412-3415.1993subject
Has Abstractpub_date
1993-08-01 00:00:00pages
3412-5issue
8eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Severe combined immunodeficient (SCID)-beige mice inoculated with the intracellular parasite Eimeria papillata produced significantly more oocysts during primary infections than inoculated immunodeficient SCID mice. Therefore, the addition of the beige mutation, which detrimentally affects neutrophil and natural kille...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3165-3170.1997
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00369-12
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.465-471.1986
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1104-1110.1979
更新日期:1979-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.368-371.1976
更新日期:1976-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02713-14
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pub_type: 杂志文章
doi:10.1128/IAI.40.2.629-632.1983
更新日期:1983-05-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2014-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.584-588.2004
更新日期:2004-01-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00585-08
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1973-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.118-126.1980
更新日期:1980-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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更新日期:2010-06-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1992-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1977-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2545-2552.1993
更新日期:1993-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
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