Abstract:
:Boyden chamber assays were performed to test the stimulatory effect of different bacterial products on the migratory activity of Mytilus haemocytes. The results indicate that these blood cells exhibit chemotactic as well as chemokinetic reactions. Lipopolysaccharides (LPS) from both Serratia marcescens and Escherichia coli stimulated the migration of cells through the membrane of the Boyden chamber when LPS was present in the lower compartment only. In contrast, addition of LPS to the lower as well as to the upper chamber did not increase the rate of migrating cells. Thus, LPS seemed to act as a chemotaxis-stimulating substance. Further analysis indicated that complete LPS molecules are required for cell stimulation because this did not occur when either the lipid or polysaccharide moieties of LPS were tested alone. Unlike LPS, the formylated tripeptide N-FMLP stimulated random cell migration. The peptide, which is released by bacteria, induced a higher haemocyte motility when present in both wells of the Boyden chamber than in tests where it was added to the lower compartment only. This chemokinetic response was not stimulated by the tetrapeptide N-FMLPLys. These findings demonstrate that bacterial products may elicit chemotactic and/or chemokinetic reactions in haemocytes from an invertebrate, and that the type of reaction that occurs is dependent upon the nature of the molecules presented.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Schneeweiss H,Renwrantz Ldoi
10.1016/0145-305x(93)90029-psubject
Has Abstractpub_date
1993-09-01 00:00:00pages
377-87issue
5eissn
0145-305Xissn
1879-0089pii
0145-305X(93)90029-Pjournal_volume
17pub_type
杂志文章abstract::The aim of this work was to identify in Eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (MTE) representative of a highly polluted smelter soil. Suppression subtractive hybridization (SSH) was used to construct cDNA libraries enriched in up- or down...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.06.009
更新日期:2008-01-01 00:00:00
abstract::A monoclonal antibody, WCL9, specific for membrane molecules of a thymocyte subpopulation was used to detect these cells in situ during the ontogeny of thymus. Cryo-sections revealed WCL9+ cells in the rudiment of the thymus (day 4 post fertilization); thereafter, the positive cells were observed exclusively in the co...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(98)00053-6
更新日期:1999-03-01 00:00:00
abstract::Tumor necrosis factor (TNF) is one of the most important cytokines involved in inflammation, apoptosis, cell proliferation, and stimulation of the immune system. The TNF gene has been cloned in teleost fish; however, the in vivo function of fish TNF is essentially unknown. In this study, we report the identification o...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2015.10.010
更新日期:2016-02-01 00:00:00
abstract::Five large multigene families encoding innate-type immune receptors that are comprised of immunoglobulin domains have been identified in bony fish, of which four do not possess definable mammalian orthologs. The members of some of the multigene families exhibit unusually extensive patterns of divergence and the indivi...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2014.02.004
更新日期:2014-09-01 00:00:00
abstract::The ingestion of sheep erythrocytes (SRBC) by echinoid phagocytes was greatly increased after treatment of SRBC with sera from mouse, human and fish. The opsonic principle in the sera was heat labile (56 degrees C 1/2 hr). Opsonization with mammalian sera was studied more extensively. It was dependent on pre-sensitiza...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(82)80028-1
更新日期:1982-07-01 00:00:00
abstract::We evaluated juvenile, pubescent, reproductive adult, and aged Japanese quail (Coturnix coturnix japonica) to determine if there were age-related differences in immune function with the hypothesis that aged birds would have weaker immune responses. Immune responses were measured using phytohemagglutinin (PHA) skin tes...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2006.07.009
更新日期:2007-01-01 00:00:00
abstract::The repertoire of gut associated T cells is shaped by exposure to microbes, including the natural enteric microflora. Previous studies compared the repertoire of gut associated T cell populations in germ free (GF) and conventional mammals often focussing on intra-epithelial lymphocyte compartments. Using GF, conventio...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2009.11.009
更新日期:2010-04-01 00:00:00
abstract::Opioid peptides are evolutionary conserved and in teleost fish their specific receptor types have been identified not only on neuroendocrine cells but also on immunocytes. In the present work we have studied the effects of morphine, ligand for the mu3 opioid receptor, on innate immune responses of common carp. Both in...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.07.004
更新日期:2009-01-01 00:00:00
abstract::Neonates of all species, including foals, are highly susceptible to infection, and neutrophils play a crucial role in innate immunity to infection. Evidence exists that neutrophils of neonatal foals are functionally deficient during the first weeks of life, including expression of cytokine genes such as IFNG. We hypot...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.06.012
更新日期:2018-10-01 00:00:00
abstract::Lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) existed in diversity of invertebrates including shrimp plays a crucial role in an innate immunity via mediating the recognition of invading pathogens. In this study, LGBP was cloned and characterized from the hepatopancreas of Litopenaeus vannamei, named as L...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.11.016
更新日期:2018-04-01 00:00:00
abstract::Pro-inflammatory cytokines are produced as part of innate immunity. Increased resistance to extraintestinal Salmonella enteritidis (SE) has been associated with an increase in heterophil pro-inflammatory cytokine gene expression. Invasion of chicken epithelial cells by SE induces an 8- to 10-fold increase in interleuk...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2004.08.002
更新日期:2005-01-01 00:00:00
abstract::Marek's disease (MD) vaccines, although effective in reducing lymphoproliferation, cannot control infectious virus production in the feather follicle epithelium (FFE) which is the site of virus shedding. Therefore, we investigated Marek's disease virus (MDV) replication as well as the expression of cytokine genes in f...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.11.003
更新日期:2009-04-01 00:00:00
abstract::In all vertebrates studied to date, the expression of MHC class II genes is known to be restricted to a limited number of tissues and cell types. In order to have a better understanding of the function of the equivalent genes in teleost fish, the distribution of MHC class II beta transcripts (Cyca-DAB) in the common c...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(95)00033-p
更新日期:1995-11-01 00:00:00
abstract::Phenoloxidase (PO) plays an important role in arthropod melanization. Previously, a prophenoloxidase (PmproPO1) gene was cloned and characterized from the hemocytes of the black tiger shrimp, Penaeus monodon. In the present study, we report a novel proPO gene (PmproPO2) belonging to the proPO family identified from th...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.09.003
更新日期:2009-02-01 00:00:00
abstract::Early activation and coordination of innate defenses are critical for effective responses against infiltrating pathogens. Rapid engagement of immune cells provides a critical first line of defense soon after pathogen infiltration. Activation leads to a well-orchestrated set of events that sees the induction and regula...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2011.03.009
更新日期:2011-12-01 00:00:00
abstract::In a dilute solution of Tris-buffered ammonium chloride (TAC), chicken erythrocytes (RBC) swell but chicken white blood cells (WBC) do not. This allowed us to separate the peaks of RBC and wBC into widely separate channels using a Coulter Counter interfaced to a microcomputer to provide size distributions. Analyses of...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(84)90044-2
更新日期:1984-04-01 00:00:00
abstract::The suppressor of cytokine signaling 1 (SOCS1) is a crucial regulator in the immune systems of mammals, which functions classically as a negatively regulator in the IFN signaling pathways. However, data on functional characterization of SOCS1 in lower vertebrates is limited. In this study, we identified and characteri...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.09.020
更新日期:2018-01-01 00:00:00
abstract::Previous studies demonstrated that porcine plasma ficolin binds the important pig pathogen Actinobacillus pleuropneumoniae (APP) in an N-acetylglucosamine-dependent manner. In the present study, attempts to characterize the bacterial-binding properties of ficolin indicated ficolin is the major porcine plasma protein t...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(03)00057-0
更新日期:2003-10-01 00:00:00
abstract::Population declines due to chytridiomycosis among frogs belonging to the Amerana (Rana boylii) species group from western North America have been particularly severe. Norepinephrine-stimulated skin secretions from the Oregon spotted frog Rana pretiosa Baird and Girard, 1853 were collected from individuals that had bee...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2011.01.017
更新日期:2011-06-01 00:00:00
abstract::As physical barriers that separate teleost fish from the external environment, mucosae are also active immunological sites that protect them against exposure to microbes and stressors. In mammals, the sites where antigens are sampled from mucosal surfaces and where stimulation of naïve T and B lymphocytes occurs are k...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2011.11.009
更新日期:2011-12-01 00:00:00
abstract::Protein(s) reactive with N-acetyl-D-glucosamine (GlcNAc) was isolated from porcine nonimmune serum. The molecular weight of the purified protein was found to be mainly 40 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. The N-terminal 10 amino acid sequence of the purified pr...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2005.04.002
更新日期:2006-01-01 00:00:00
abstract::CD42 is a platelet membrane glycoprotein Ib that plays a key role in haemostasis and thrombin-induced platelet activation. Here, we report the molecular cloning and sequence analysis of the CD42c gene from rock bream (Oplegnathus fasciatus). Rock bream CD42 (RbCD42c) gene expression profiles were determined after infe...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2014.11.011
更新日期:2015-04-01 00:00:00
abstract::A comprehensive analysis of cattle shotgun sequencing data reveals 36 immunoglobulin heavy chain variable genes. The previously described bovine subgroup IGHV1 contains 10 functional genes with a conserved promoter including the consensus octamer and several other transcription factor binding sites, intact exons and m...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.02.006
更新日期:2012-07-01 00:00:00
abstract::B lymphocyte-induced maturation protein 1 (Blimp-1), a transcription factor containing zinc finger, is required and sufficient to trigger terminal differentiation of B cells in mammals. The Blimp-1 (OnBlimp-1) from Nile tilapia (Oreochromis niloticus) was identified and characterized its expression pattern during B ce...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2019.05.002
更新日期:2019-09-01 00:00:00
abstract::In 1954 the discovery of bursal function was one of the major contributions to the formation of the T and B cell concept of immunology. In 1978 the avian dendritic cells; bursal secretory dendritic cell (BSDC) and follicular dendritic cell (FDC) in the cecal tonsil were recognized. In 1982 the interdigitating dendriti...
journal_title:Developmental and comparative immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.dci.2013.03.007
更新日期:2013-11-01 00:00:00
abstract::Invertebrates utilise the innate immune system when defending against pathogenic attack. However, except for some effectors as proPhenolOxidase (proPO), the innate immune response is less well understood outside model insect species, and its role in natural host-pathogen systems is generally not well documented. We ha...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.11.012
更新日期:2009-05-01 00:00:00
abstract::Anti-lipopolysaccharide factor (ALF) is an antimicrobial peptide originally identified from horseshoe crabs and recently found in several shrimp species. ALFPm3, the most abundant isoform in the black tiger shrimp, Penaeus monodon, has been shown to possess a broad spectrum of antimicrobial activity against Gram-negat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.03.008
更新日期:2008-01-01 00:00:00
abstract::In order to find the immune-relevant factors responsible for the virus resistance in the WSSV-resistant shrimp, a suppression subtractive hybridization method was employed to identify differentially expressed genes and their expression profile in the hepatopancreas of the virus-resistant penaeid shrimp. Thirty five ge...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2004.07.001
更新日期:2005-01-01 00:00:00
abstract::The skin mucus of the conger eel, Conger myriaster, contains galactose-specific agglutinins. Hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 degrees C for 15 min. The mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. Three of...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(88)90007-9
更新日期:1988-04-01 00:00:00
abstract::Diapausing pupae of Cecropia respond to a bacterial infection by the selective synthesis of RNA and 15-20 hemolymph proteins. Of these we have purified lysozyme and two classes of antibacterial proteins called cecropins and attacins. The primary structure has been determined for the lysozyme, one attacin and five cecr...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(85)90018-7
更新日期:1985-07-01 00:00:00