Regulatory effects of matrix protein variations on influenza virus growth.

Abstract:

:Influenza virus A/WSN/33 forms large plaques (> 3 mm diameter) on MDCK cells whereas A/Aichi/2/68 forms only small plaques (< 1 mm diameter). Fast growing reassortants (AWM), isolated by mixed infection of MDCK cells with these two virus strains in the presence of anti-WSN antibodies, all carried the M gene from WSN. On MDCK cells, these reassortants produced progeny viruses as rapidly as did WSN, and the virus yield was as high as Aichi. The fast-growing reassortants overcame the growth inhibitory effect of lignins. Pulse-labeling experiments at various times after virus infection showed that the reassortant AWM started to synthesize viral proteins earlier than Aichi. Taken together, we conclude that upon infecting MDCK cells, the reassortant viruses advance rapidly into the growth cycle, thereby leading to an elevated level of progeny viruses in the early period of infection. Possible mechanisms of the M gene involvement in the determination of virus growth rate are discussed, in connection with multiple functions of the M proteins.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Yasuda J,Toyoda T,Nakayama M,Ishihama A

doi

10.1007/BF01313769

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

283-94

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

133

pub_type

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