Abstract:
:We have previously identified a self-reactive gamma delta T-cell clone (KN6) specific for the H-2T region gene product T22b. Now we have investigated by an in vitro mutagenesis analysis of the T22b gene the possibility that the interaction between the KN6 gamma delta T-cell receptor and T22b involves a peptide. The results demonstrate that mutations at the floor of the putative antigen-binding groove of T22b affect recognition by the gamma delta T-cell receptor. Furthermore, we have shown that KN6 cells react with cells that are deficient in the class I peptide transporter TAP1/TAP2. These results suggest that peptide is involved in the interaction of the KN6 T-cell receptor with T22 and that loading of T22 with the putative peptide is TAP1/TAP2-independent.
journal_name
Proc Natl Acad Sci U S Aauthors
Moriwaki S,Korn BS,Ichikawa Y,van Kaer L,Tonegawa Sdoi
10.1073/pnas.90.23.11396subject
Has Abstractpub_date
1993-12-01 00:00:00pages
11396-400issue
23eissn
0027-8424issn
1091-6490journal_volume
90pub_type
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