Detection of an altered DNA conformation at specific sites in chromatin and supercoiled DNA.

abstract：:The fundamental questions of how lubricant molecules organize into a layered structure under nanometers confinement and what is the interplay between layering and friction are still not well answered in the field of nanotribology. While the phase transition of lubricants during a squeeze-out process under compression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu RG,Leng Y

更新日期：2018-06-26 00:00:00

abstract：:A strong mutator effect has been observed in Escherichia coli K-12 strains mutated in the bglY gene (27 min). The frequency of point mutations is not modified in bglY mutant strains. In contrast, a strong increase in spontaneous generation of large deletions has been observed in these strains, both for chromosomal mar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lejeune P,Danchin A

更新日期：1990-01-01 00:00:00

abstract：:Histone mRNAs are naturally intronless and accumulate efficiently in the cytoplasm. To learn whether there are cis-acting sequences within histone genes that allow efficient cytoplasmic accumulation of RNAs, we made recombinant constructs in which sequences from the mouse H2a gene were cloned into a human beta-globin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang Y,Carmichael GG

更新日期：1997-09-16 00:00:00

abstract：:An alpha-bungarotoxin-horseradish peroxidase conjugate, which binds specificity to nicotinic acetylcholine receptors, was synthesized. This conjugate was bound by 5-7% of the synapses in the inner plexiform layer of the chicken retina. Bipolar ribbon synapses as well as amacrine synapses bound the conjugate. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogel Z,Maloney GJ,Ling A,Daniels MP

更新日期：1977-08-01 00:00:00

abstract：:Fifty cats with feline leukemia virus (FeLV) infection and leukemia-lymphoma complex were treated by ex vivo immunoadsorption with Staphylococcus protein A-bound filters. Most cats responded to therapy. Twelve showed tumor regression, including disappearance of tumor cells, but died later of other complications. Three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu WT,Engelman RW,Trang LQ,Hau K,Good RA,Day NK

更新日期：1984-06-01 00:00:00

abstract：:The importance of core histones in the regulation of DNA function by chromatin is clear. However, little is known about the role of the linker histone. We investigated the role of H1 in Saccharomyces cerevisiae during extensive transcriptional reprogramming in stationary phase. Although the levels of linker histone Hh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schäfer G,McEvoy CR,Patterton HG

更新日期：2008-09-30 00:00:00

abstract：:The very low density lipoprotein (VLDL) receptor is a recently cloned member of the low density lipoprotein (LDL) receptor family that mediates the binding and uptake of VLDL when overexpressed in animal cells. Its sequence is 94% identical in humans and rabbits and 84% identical in humans and chickens, implying a con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frykman PK,Brown MS,Yamamoto T,Goldstein JL,Herz J

更新日期：1995-08-29 00:00:00

abstract：:DNA fragmentation is a hallmark of apoptosis (programmed cell death). However, the biological function of apoptotic DNA fragmentation remains unclear. Here, we show that DNA fragmentation factor plays an important role for maintaining genomic stability. Inhibition or loss of the DNA fragmentation factor (DFF)/caspase-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan B,Wang H,Peng Y,Hu Y,Wang H,Zhang X,Chen Q,Bedford JS,Dewhirst MW,Li CY

更新日期：2006-01-31 00:00:00

abstract：:Goblet-cell hyperplasia is a critical pathological feature in hypersecretory diseases of airways. However, the underlying mechanisms are unknown, and no effective therapy exists. Here we show that stimulation of epidermal growth factor receptors (EGF-R) by its ligands, EGF and transforming growth factor alpha (TGFalph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takeyama K,Dabbagh K,Lee HM,Agustí C,Lausier JA,Ueki IF,Grattan KM,Nadel JA

更新日期：1999-03-16 00:00:00

abstract：:Molecular recognition by proteins is fundamental to molecular biology. Dissection of the thermodynamic energy terms governing protein-ligand interactions has proven difficult, with determination of entropic contributions being particularly elusive. NMR relaxation measurements have suggested that changes in protein con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caro JA,Harpole KW,Kasinath V,Lim J,Granja J,Valentine KG,Sharp KA,Wand AJ

更新日期：2017-06-20 00:00:00

abstract：:Many substances in the plant kingdom and in man's diet occur as glycosides. Recent studies have indicated that many glycosides that are not mutagenic in tests such as the Salmonella test become mutagenic upon hydrolysis of the glycosidic linkages. The Salmonella test utilizes a liver homogenate to approximate mammalia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tamura G,Gold C,Ferro-Luzzi A,Ames BN

更新日期：1980-08-01 00:00:00

abstract：:Systemic inflammation occurring around the course of tumor progression and treatment are often correlated with adverse oncological outcomes. As such, it is suspected that neutrophils, the first line of defense against infection, may play important roles in linking inflammation and metastatic seeding. To decipher the d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen MB,Hajal C,Benjamin DC,Yu C,Azizgolshani H,Hynes RO,Kamm RD

更新日期：2018-07-03 00:00:00

abstract：:Hereditary hemochromatosis (HH) is a common chronic human genetic disorder whose hallmark is systemic iron overload. Homozygosity for a mutation in the MHC class I heavy chain paralogue gene HFE has been found to be a primary cause of HH. However, many individuals homozygous for the defective allele of HFE do not deve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sproule TJ,Jazwinska EC,Britton RS,Bacon BR,Fleming RE,Sly WS,Roopenian DC

更新日期：2001-04-24 00:00:00

abstract：:Experimental systemic lupus erythematosus (SLE) can be induced in naive mice by immunization with a murine monoclonal anti-DNA antibody (mAb), 5G12, that bears a major idiotype designated 16/6 Id. Strain-dependent differences were observed in the proliferative responses of lymph node cells of mice immunized with two p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waisman A,Ruiz PJ,Israeli E,Eilat E,Könen-Waisman S,Zinger H,Dayan M,Mozes E

更新日期：1997-04-29 00:00:00

abstract：:There is now abundant evidence to substantiate an important role of hepatitis C virus (HCV) core protein in cellular gene expression as well as in the viral cycle. Thus the subcellular localization of this protein has important implications. However, several studies have shown controversial results: the HCV core has b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barba G,Harper F,Harada T,Kohara M,Goulinet S,Matsuura Y,Eder G,Schaff Z,Chapman MJ,Miyamura T,Bréchot C

更新日期：1997-02-18 00:00:00

abstract：:To determine whether the human pituitary contains a previously unidentified, nonprolactin (non-hPRL), non-growth-hormone (non-hGH) factor capable of stimulating mammary development, we tested the effects of whole human pituitary extract (hPE) and pituitary extracts depleted of hPRL and hGH ("stripped hPE") in hypophys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newman CB,Cosby H,Friesen HG,Feldman M,Cooper P,De Crescito V,Pilon M,Kleinberg DL

更新日期：1987-11-01 00:00:00

abstract：:The gene that codes for Drosophila alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase EC 1.1.1.1) was identified in a bacteriophage lambda library of genomic Drosophila DNA by using ADH cDNA cloned DNA as a probe. The DNA sequence of the protein encoding region was shown to be in agreement with the amino acid seq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benyajati C,Place AR,Powers DA,Sofer W

更新日期：1981-05-01 00:00:00

abstract：:Mutations are introduced into rearranged Ig variable genes at a frequency of 10(-2) mutations per base pair by an unknown mechanism. Assuming that DNA repair pathways generate or remove mutations, the frequency and pattern of mutation will be different in variable genes from mice defective in repair. Therefore, hyperm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winter DB,Phung QH,Umar A,Baker SM,Tarone RE,Tanaka K,Liskay RM,Kunkel TA,Bohr VA,Gearhart PJ

更新日期：1998-06-09 00:00:00

abstract：:A translocation involving human chromosome 10, band q24, in a subset of T-cell acute leukemias disrupts a region surrounding the putative oncogene HOX11, which encodes a protein with a homeodomain. The HOX11 protein binds to a specific DNA sequence, it localizes to the cell nucleus, and it transactivates transcription...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dear TN,Sanchez-Garcia I,Rabbitts TH

更新日期：1993-05-15 00:00:00

abstract：:The zinc finger motif, exemplified by a segment of the Drosophila gap gene Krüppel, is a nucleic acid-binding domain present in many transcription factors. To investigate the gene family encoding this motif in the human genome, a placental genomic library was screened at moderate stringency with a degenerate oligodeox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bray P,Lichter P,Thiesen HJ,Ward DC,Dawid IB

更新日期：1991-11-01 00:00:00

abstract：:The importance of Gly-93 and Gly-94 in transmembrane segment M1 of the Na+,K+-ATPase for interaction with Na+ and K+ was demonstrated by functional analysis of mutants Gly-93-Ala and Gly-94-Ala. In the crystal structures of the Ca2+-ATPase, the corresponding residues, Asp-59 and Leu-60, are located exactly where M1 be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Einholm AP,Toustrup-Jensen M,Andersen JP,Vilsen B

更新日期：2005-08-09 00:00:00

abstract：:Mechanisms that enabled primitive cell membranes to self-reproduce have been discussed based on the physicochemical properties of fatty acids; however, there must be a transition to modern cell membranes composed of phospholipids [Budin I, Szostak JW (2011) Proc Natl Acad Sci USA 108:5249-5254]. Thus, a growth-divisio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Terasawa H,Nishimura K,Suzuki H,Matsuura T,Yomo T

更新日期：2012-04-17 00:00:00

abstract：:Cellular differentiation leading to formation of the bradyzoite tissue cyst stage is the underlying cause of chronic toxoplasmosis. Consequently, mechanisms responsible for controlling development in the Toxoplasma intermediate life cycle have long been sought. Here, we identified 15 Toxoplasma mRNAs induced in early ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Radke JB,Lucas O,De Silva EK,Ma Y,Sullivan WJ Jr,Weiss LM,Llinas M,White MW

更新日期：2013-04-23 00:00:00

abstract：:Spontaneous liposome formation is predicted in binary mixtures of fluid phase phospholipids and poly(n)ethylene oxide (PEO)-bearing lipids by using single chain mean field theory. The range of stability of the spontaneous liposomes is determined as a function of percentage of PEO-conjugated lipids and polymer molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szleifer I,Gerasimov OV,Thompson DH

更新日期：1998-02-03 00:00:00

abstract：:Drug-target residence time (t = 1/k(off), where k(off) is the dissociation rate constant) has become an important index in discovering better- or best-in-class drugs. However, little effort has been dedicated to developing computational methods that can accurately predict this kinetic parameter or related parameters, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bai F,Xu Y,Chen J,Liu Q,Gu J,Wang X,Ma J,Li H,Onuchic JN,Jiang H

更新日期：2013-03-12 00:00:00

abstract：:Social separation early in life can lead to the development of impaired interpersonal relationships and profound social disorders. However, the underlying cellular and molecular mechanisms involved are largely unknown. Here, we found that isolation of neonatal rats induced glucocorticoid-dependent social dominance ove...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tada H,Miyazaki T,Takemoto K,Takase K,Jitsuki S,Nakajima W,Koide M,Yamamoto N,Komiya K,Suyama K,Sano A,Taguchi A,Takahashi T

更新日期：2016-11-08 00:00:00

abstract：:The assembly of heterogeneous nuclear RNA (hnRNA) into ribonucleoprotein (RNP) particles has been investigated during in vitro transcription in isolated nuclei. Approximately 80% of the in vitro transcription observed in mouse Friend erythroleukemia cell nuclei is attributable to the activity of RNA polymerase II. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Economidis IV,Pederson T

更新日期：1982-03-01 00:00:00

abstract：:In contrast to observations in bacteria and fungi, frameshift mutations in bacteriophage T4 do not arise during genetic recombination. Nascent mutants, captured in the heterozygous condition, exhibit properties which indicate that the new lesions are located at the extreme tips of the chromosomes, and are segregated b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindstrom DM,Drake JW

更新日期：1970-03-01 00:00:00

abstract：:A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo sit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kopecko DJ,Cohen SN

更新日期：1975-04-01 00:00:00

abstract：:LINEs are transposable elements found in various eukaryotes such as plants, protists, insects, and mammals. Their transposition is usually difficult to study, particularly in humans, where some diseases have been shown to result from LINE insertion mutations. This is due to the fact that most copies of any particular ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pélisson A,Finnegan DJ,Bucheton A

更新日期：1991-06-01 00:00:00