Entropy in molecular recognition by proteins.

abstract：:Marked hypoalphalipoproteinemia was found together with relatively low serum cholesterol, triacylglycerol, and LDL levels in a triose-phosphate isomerase (TPI; D-glyceraldehyde-3-phosphate ketol-isomerase, EC 5.3.1.1)-deficient Hungarian family, especially in the two compound-heterozygote brothers. Apart from a slight...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hollán S,Dey I,Szollár L,Horányi M,Magócsi M,Harsányi V,Farkas T

更新日期：1995-01-03 00:00:00

abstract：:We investigated the effects of beta-estradiol on the locomotor behavior of female mice in a radial maze. Data comprising the total distance traveled during each arm entry were obtained from video records of six consecutive daily recording sessions. Distributions of these data were bimodal for both ovariectomized contr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coop AD,Stavarache MA,Pfaff DW,Reeke GN

更新日期：2006-10-17 00:00:00

abstract：:We have developed a technology for analysis and sorting of live cells according to secreted molecules. An artificial affinity matrix, specific for the secreted product of interest, is created on the cell surface, and the cells are allowed to secrete for a defined time period. The secreted molecules bind to the affinit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manz R,Assenmacher M,Pflüger E,Miltenyi S,Radbruch A

更新日期：1995-03-14 00:00:00

abstract：:The mahogany (mg) locus originally was identified as a recessive suppressor of agouti, a locus encoding a skin peptide that modifies coat color by antagonizing the melanocyte-stimulating hormone receptor or MC1-R. Certain dominant alleles of agouti cause an obesity syndrome when ectopic expression of the peptide aberr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dinulescu DM,Fan W,Boston BA,McCall K,Lamoreux ML,Moore KJ,Montagno J,Cone RD

更新日期：1998-10-13 00:00:00

abstract：:A physical map of the 9.2-Mbp Myxococcus xanthus DK1622 chromosome at a resolution of 25 kbp was constructed by using a strategy that is applicable to virtually all microorganisms. Segments of the chromosome were used as hybridization probes to subdivide a yeast artificial chromosome (YAC) library into groups of linke...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He Q,Chen H,Kuspa A,Cheng Y,Kaiser D,Shimkets LJ

更新日期：1994-09-27 00:00:00

abstract：:We describe a new computer program, trilogy, for the automated discovery of sequence-structure patterns in proteins. trilogy implements a pattern discovery algorithm that begins with an exhaustive analysis of flexible three-residue patterns; a subset of these patterns are selected as seeds for an extension process in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bradley P,Kim PS,Berger B

更新日期：2002-06-25 00:00:00

abstract：:Aminoacyl-tRNA synthetases (amino acid-tRNA ligases, EC 6.1.1.-) catalyze the aminoacylation of specific amino acids onto their cognate tRNAs with extraordinary accuracy. Recent reports, however, indicate that this class of enzymes may play other roles in cellular metabolism. Several aminoacyl-tRNA synthetases are her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rapaport E,Remy P,Kleinkauf H,Vater J,Zamecnik PC

更新日期：1987-11-01 00:00:00

abstract：:We consider many-body problems in classical mechanics where a wide range of time scales limits what can be computed. We apply the method of optimal prediction to obtain equations that are easier to solve numerically. We demonstrate by examples that optimal prediction can reduce the amount of computation needed to obta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kast AP

更新日期：2000-06-06 00:00:00

abstract：:Notwithstanding important advances in the context of single-variant pathogenicity identification, novel breakthroughs in discerning the origins of many rare diseases require methods able to identify more complex genetic models. We present here the Variant Combinations Pathogenicity Predictor (VarCoPP), a machine-learn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papadimitriou S,Gazzo A,Versbraegen N,Nachtegael C,Aerts J,Moreau Y,Van Dooren S,Nowé A,Smits G,Lenaerts T

更新日期：2019-06-11 00:00:00

abstract：:Total chemical synthesis was used to prepare the mirror image (D-protein) form of the angiogenic protein vascular endothelial growth factor (VEGF-A). Phage display against D-VEGF-A was used to screen designed libraries based on a unique small protein scaffold in order to identify a high affinity ligand. Chemically syn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mandal K,Uppalapati M,Ault-Riché D,Kenney J,Lowitz J,Sidhu SS,Kent SB

更新日期：2012-09-11 00:00:00

abstract：:A simple method has been developed for fusing synaptic vesicles into spherical structures 20-50 micron in diameter. The method has been applied to purified cholinergic synaptic vesicles from Torpedo electric organ, and the membrane properties of these fused structures have been studied by the "cell"-attached version o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rahamimoff R,DeRiemer SA,Sakmann B,Stadler H,Yakir N

更新日期：1988-07-01 00:00:00

abstract：:We have combined standard micrococcal nuclease (MNase) digestion of nuclei with a modified protocol for constructing paired-end DNA sequencing libraries to map both nucleosomes and subnucleosome-sized particles at single base-pair resolution throughout the budding yeast genome. We found that partially unwrapped nucleo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henikoff JG,Belsky JA,Krassovsky K,MacAlpine DM,Henikoff S

更新日期：2011-11-08 00:00:00

abstract：:Memory is stored in neural networks via changes in synaptic strength mediated in part by NMDA receptor (NMDAR)-dependent long-term potentiation (LTP). Here we show that a cholecystokinin (CCK)-B receptor (CCKBR) antagonist blocks high-frequency stimulation-induced neocortical LTP, whereas local infusion of CCK induces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Li X,Wong YT,Zheng X,Wang H,Peng Y,Feng H,Feng J,Baibado JT,Jesky R,Wang Z,Xie H,Sun W,Zhang Z,Zhang X,He L,Zhang N,Zhang Z,Tang P,Su J,Hu LL,Liu Q,He X,Tan A,Sun X,Li M,Wong K,Wang X,Cheung

更新日期：2019-03-26 00:00:00

abstract：:Dead and dying cells release nucleic acids. These extracellular RNAs and DNAs can be taken up by inflammatory cells and activate multiple nucleic acid-sensing toll-like receptors (TLR3, 7, 8, and 9). The inappropriate activation of these TLRs can engender a variety of inflammatory and autoimmune diseases. The redundan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee J,Sohn JW,Zhang Y,Leong KW,Pisetsky D,Sullenger BA

更新日期：2011-08-23 00:00:00

abstract：:The specific nucleocytoplasmic compartmentalization of proteins has been examined for some major soluble acidic nuclear proteins in oocytes of different amphibia. Proteins synthesized and radioactively labeled by translation in vitro, by using mRNA from ovaries of the frog Xenopus laevis, were injected into the cytopl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dabauvalle MC,Franke WW

更新日期：1982-09-01 00:00:00

abstract：:cGMP is a key regulatory molecule in visual transduction, integration of neuronal response to excitatory neurotransmitters, relaxation of smooth muscle, intestinal secretion of water and salt, and reabsorption of sodium and water in the distal tubules of the nephron. Some of these cellular functions are associated wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruth P,Wang GX,Boekhoff I,May B,Pfeifer A,Penner R,Korth M,Breer H,Hofmann F

更新日期：1993-04-01 00:00:00

abstract：:Previously, we reported that bilateral excitotoxic lesions of the basolateral nucleus of the amygdala (BLA) block the enhancing effects of posttraining systemic or intrahippocampal glucocorticoid administration on memory for inhibitory avoidance training. The present study further examined the basis of this permissive...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roozendaal B,Nguyen BT,Power AE,McGaugh JL

更新日期：1999-09-28 00:00:00

abstract：:The Paleocene-Eocene Thermal Maximum (PETM) (∼56 Ma) was a ∼170,000-y (∼170-kyr) period of global warming associated with rapid and massive injections of 13C-depleted carbon into the ocean-atmosphere system, reflected in sedimentary components as a negative carbon isotope excursion (CIE). Carbon cycle modeling has ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frieling J,Svensen HH,Planke S,Cramwinckel MJ,Selnes H,Sluijs A

更新日期：2016-10-25 00:00:00

abstract：:Granulocyte-colony-stimulating factor (G-CSF) stimulates the proliferation and differentiation of cells of the neutrophil lineage by interaction with a specific receptor. Early signal transduction events following G-CSF receptor activation were studied. We detected tyrosine phosphorylation of both the G-CSF receptor a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicholson SE,Oates AC,Harpur AG,Ziemiecki A,Wilks AF,Layton JE

更新日期：1994-04-12 00:00:00

abstract：:Copper is an essential micronutrient that plays a central role for a broad range of biological processes. Although there is compelling evidence that the intracellular milieu does not contain any free copper ions, the rapid kinetics of copper uptake and release suggests the presence of a labile intracellular copper poo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang L,McRae R,Henary MM,Patel R,Lai B,Vogt S,Fahrni CJ

更新日期：2005-08-09 00:00:00

abstract：:The family of WD40-repeat (WDR) proteins is one of the largest in eukaryotes, but little is known about their function in brain development. Among 26 WDR genes assessed, we found 7 displaying a major impact in neuronal morphology when inactivated in mice. Remarkably, all seven genes showed corpus callosum defects, inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kannan M,Bayam E,Wagner C,Rinaldi B,Kretz PF,Tilly P,Roos M,McGillewie L,Bär S,Minocha S,Chevalier C,Po C,Sanger Mouse Genetics Project.,Chelly J,Mandel JL,Borgatti R,Piton A,Kinnear C,Loos B,Adams DJ,Hérault Y,

更新日期：2017-10-31 00:00:00

abstract：:SoxR is a transcription factor that governs a global defense against the oxidative stress caused by nitric oxide or excess superoxide in Escherichia coli. SoxR is a homodimer containing a pair of [2Fe-2S] clusters essential for its transcriptional activity, and changes in the stability of these metal centers could con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ding H,Demple B

更新日期：1996-09-03 00:00:00

abstract：:The sloppy paired (slp) locus consists of two genes, slp1 and slp2, both of which encode proteins containing a forkhead domain (a DNA-binding motif). Previous work has shown that a severe segmentation phenotype is obtained only when both slp genes are deleted. Here we examine the functional redundancy of the locus in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cadigan KM,Grossniklaus U,Gehring WJ

更新日期：1994-07-05 00:00:00

abstract：:We have cloned a mutS homolog from Drosophila melanogaster called spellchecker1 (spel1) and have constructed spel1 mutant flies. MutS proteins promote the correction of DNA mismatches and serve important roles in DNA replication, recombination, and repair. The spel1 gene belongs to a subfamily of mutS first characteri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flores C,Engels W

更新日期：1999-03-16 00:00:00

abstract：:Society makes substantial investments in biomedical research, searching for ways to better human health. The product of this research is principally information published in scientific journals. Continued investment in science relies on society's confidence in the accuracy, honesty, and utility of research results. A ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowen A,Casadevall A

更新日期：2015-09-08 00:00:00

abstract：:Viruses modulate biochemical cellular pathways to permit infection. A recently described mechanism mediates selective protein interactions between acidic domain readers and unacetylated, lysine-rich regions, opposite of bromodomain function. Kaposi´s sarcoma (KS)-associated herpesvirus (KSHV) is tightly linked with KS...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Juillard F,de Miranda MP,Li S,Franco A,Seixas AF,Liu B,Álvarez ÁL,Tan M,Szymula A,Kaye KM,Simas JP

更新日期：2020-09-08 00:00:00

abstract：:Induction, maintenance, and amplification of tumor-protective immunity after cytokine gene therapy is essential for the clinical success of immunotherapeutic approaches. We investigated whether this could be achieved by single-chain IL-12 (scIL-12) gene therapy followed by tumor-targeted IL-2 using a fusion protein co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lode HN,Xiang R,Duncan SR,Theofilopoulos AN,Gillies SD,Reisfeld RA

更新日期：1999-07-20 00:00:00

abstract：:Disordered systems like liquids, gels, glasses, or granular materials are not only ubiquitous in daily life and in industrial applications, but they are also crucial for the mechanical stability of cells or the transport of chemical and biological agents in living organisms. Despite the importance of these systems, th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Z,Kob W

更新日期：2020-06-23 00:00:00

abstract：:Monoclonal antibodies have been obtained that recognize either the A or B chain of human urinary urokinase. These antibodies identify human urokinase-producing cells and the product of urokinase mRNA. Anti-urokinase monoclonal antibodies precipitate an approximately equal to 54,000-dalton protein synthesized in vitro ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salerno G,Verde P,Nolli ML,Corti A,Szöts H,Meo T,Johnson J,Bullock S,Cassani G,Blasi F

更新日期：1984-01-01 00:00:00

abstract：:Epidemiological and genetic association studies show that genetics play an important role in the attainment of education. Here, we investigate the effect of this genetic component on the reproductive history of 109,120 Icelanders and the consequent impact on the gene pool over time. We show that an educational attainm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kong A,Frigge ML,Thorleifsson G,Stefansson H,Young AI,Zink F,Jonsdottir GA,Okbay A,Sulem P,Masson G,Gudbjartsson DF,Helgason A,Bjornsdottir G,Thorsteinsdottir U,Stefansson K

更新日期：2017-01-31 00:00:00