Chimeric plant calcium/calmodulin-dependent protein kinase gene with a neural visinin-like calcium-binding domain.

abstract：:A transient transfection assay using Drosophila S2 tissue culture cells and WT and mutant Drosophila vnd/NK-2 homeobox cDNAs was used to localize repression and activation domains of vnd/NK-2 homeodomain protein. A repression domain was identified near the N terminus of vnd/NK-2 homeodomain protein (amino acid residue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stepchenko A,Nirenberg M

更新日期：2004-09-07 00:00:00

abstract：:Transcription factors (TFs) are primary regulators of gene expression in cells, where they bind specific genomic target sites to control transcription. Quantitative measurements of TF-DNA binding energies can improve the accuracy of predictions of TF occupancy and downstream gene expression in vivo and shed light on h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le DD,Shimko TC,Aditham AK,Keys AM,Longwell SA,Orenstein Y,Fordyce PM

更新日期：2018-04-17 00:00:00

abstract：:Graphene oxide (GO), the main precursor of graphene-based materials made by solution processing, is known to be very stiff. Indeed, it has a Young's modulus comparable to steel, on the order of 300 GPa. Despite its very high stiffness, we show here that GO is superflexible. We quantitatively measure the GO bending rig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poulin P,Jalili R,Neri W,Nallet F,Divoux T,Colin A,Aboutalebi SH,Wallace G,Zakri C

更新日期：2016-10-04 00:00:00

abstract：:Silencing of multiple cancer-related genes is associated with de novo methylation of linked CpG islands. Additionally, bivalent histone modification profiles characterized by the juxtaposition of active and inactive histone marks have been observed in genes that become hypermethylated in cancer. It is unknown how thes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rodriguez J,Muñoz M,Vives L,Frangou CG,Groudine M,Peinado MA

更新日期：2008-12-16 00:00:00

abstract：:Avian reovirus S1133 penetrates and uncoats in suspension cultures of mouse L cells. The multiple species of viral transcripts are produced in the cytoplasm of the infected cell, but they fail to associate with polysomes, consistent with the absence of viral protein synthesis. The selective block in avian virus mRNA t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benavente J,Shatkin AJ

更新日期：1988-06-01 00:00:00

abstract：:Avian leukosis virus subgroup J (ALV-J) is an important concern for the poultry industry. Replication of ALV-J depends on a functional cellular receptor, the chicken Na+/H+ exchanger type 1 (chNHE1). Tryptophan residue number 38 of chNHE1 (W38) in the extracellular portion of this molecule is a critical amino acid for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koslová A,Trefil P,Mucksová J,Reinišová M,Plachý J,Kalina J,Kučerová D,Geryk J,Krchlíková V,Lejčková B,Hejnar J

更新日期：2020-01-28 00:00:00

abstract：:Leukemia cell lines of the monocytic series (HL-60, U-937, and P388D1) produce a hepatocyte-stimulating factor (HSF) following induction of differentiation with phorbol diester. In 24-72 hr, these leukemia cells produce 2-30% the amount of HSF as human peripheral blood monocytes. Cells of the series at earlier stages ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woloski BM,Fuller GM

更新日期：1985-03-01 00:00:00

abstract：:Creative cognition requires mental exploration of remotely connected concepts while suppressing dominant ones. Across four experiments using different samples of participants, we provide evidence that right temporal alpha oscillations play a crucial role in inhibiting habitual thinking modes, thereby paving the way fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luft CDB,Zioga I,Thompson NM,Banissy MJ,Bhattacharya J

更新日期：2018-12-26 00:00:00

abstract：:Protein-binding DNA sequence elements encode a variety of regulated functions of genomes. Information about such elements is currently in a state of rapid growth, but improved methods are required to characterize the sequence specificity of DNA-binding proteins. We have established an in vitro method for specific and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gustafsdottir SM,Schlingemann J,Rada-Iglesias A,Schallmeiner E,Kamali-Moghaddam M,Wadelius C,Landegren U

更新日期：2007-02-27 00:00:00

abstract：:Ras is a guanine nucleotide-binding protein that cycles between inactive GDP-bound and active GTP-bound states to regulate a diverse array of cellular processes, including cell growth, apoptosis, and differentiation. The guanine nucleotide-bound state of Ras is tightly maintained by regulatory factors to promote regul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams JG,Pappu K,Campbell SL

更新日期：2003-05-27 00:00:00

abstract：:Hebb proposed that neuronal cell assemblies are critical for effective perception, cognition, and action. However, evidence for brain mechanisms that coordinate multiple coactive assemblies remains lacking. Neuronal oscillations have been suggested as one possible mechanism for cell assembly coordination. Prior studie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Canolty RT,Ganguly K,Kennerley SW,Cadieu CF,Koepsell K,Wallis JD,Carmena JM

更新日期：2010-10-05 00:00:00

abstract：:Gonadal steroids have traditionally been found to have short-term effects on the mating behavior of adult vertebrates, such that increased steroid hormone levels influence behavior over the course of a few days. Long-term effects also have been observed in which embryonic exposure to steroid hormone influences the sex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crews D

更新日期：1991-05-01 00:00:00

abstract：:The mitochondrial uniporter is a Ca2+-channel complex resident within the organelle's inner membrane. In mammalian cells the uniporter's activity is regulated by Ca2+ due to concerted action of MICU1 and MICU2, two paralogous, but functionally distinct, EF-hand Ca2+-binding proteins. Here we present the X-ray structur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamer KJ,Jiang W,Kaushik VK,Mootha VK,Grabarek Z

更新日期：2019-02-26 00:00:00

abstract：:High-throughput screening (HTS) of chemical compounds to identify modulators of molecular targets is a mainstay of pharmaceutical development. Increasingly, HTS is being used to identify chemical probes of gene, pathway, and cell functions, with the ultimate goal of comprehensively delineating relationships between ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inglese J,Auld DS,Jadhav A,Johnson RL,Simeonov A,Yasgar A,Zheng W,Austin CP

更新日期：2006-08-01 00:00:00

abstract：:We previously identified rat8 in the pathway involved in epithelial cell differentiation that occurs in the rat mammary gland at pregnancy when tubules and alveoli are formed. rat8, which encodes an IFN-inducible membrane protein, is the rat homologue of the mouse gene fragilis. By differential detergent extraction an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zucchi I,Prinetti A,Scotti M,Valsecchi V,Valaperta R,Mento E,Reinbold R,Vezzoni P,Sonnino S,Albertini A,Dulbecco R

更新日期：2004-02-17 00:00:00

abstract：:New experiments using scanning probe microcopies and advanced optical methods allow us to study molecules as individuals, not just as populations. The findings of these studies not only include the confirmation of results expected from studies of bulk matter, but also give substantially new information concerning the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bai C,Wang C,Xie XS,Wolynes PG

更新日期：1999-09-28 00:00:00

abstract：:Natural killer (NK) cells play a critical role in controlling malignancies. Susceptibility or resistance to lung cancer, for example, specifically depends on NK cell function. Nevertheless, intrinsic factors that control NK cell-mediated clearance of lung cancer are unknown. Here we report that NK cells exposed to exo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi L,Li K,Guo Y,Banerjee A,Wang Q,Lorenz UM,Parlak M,Sullivan LC,Onyema OO,Arefanian S,Stelow EB,Brautigan DL,Bullock TNJ,Brown MG,Krupnick AS

更新日期：2018-11-13 00:00:00

abstract：:Chromosome rearrangements, such as large deletions, inversions, or translocations, mediate migration of large DNA segments within or between chromosomes, which can have major effects on cellular genetic control. A method for chromosome manipulation would be very useful for studying the consequences of large-scale DNA ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Deursen J,Fornerod M,Van Rees B,Grosveld G

更新日期：1995-08-01 00:00:00

abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a C-terminal hydrophilic domain that is homologous to IIA protein(s) domains of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). C-terminal truncation mutants were constructed and expressed in Escherichia coli and their properties we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poolman B,Knol J,Mollet B,Nieuwenhuis B,Sulter G

更新日期：1995-01-31 00:00:00

abstract：:A mutant (ML 308-22) was isolated from Escherichia coli ML 308, which had lost the normal capacity to accumulate lactose analogs despite an increase in the membrane carrier activity. The exit of thiomethylgalactoside was much faster than normal, accounting for the inability of the cell to maintain high intracellular c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong PT,Kashket ER,Wilson TH

更新日期：1970-01-01 00:00:00

abstract：:Arginine is an amino acid that serves as a substrate for nitric oxide synthase and arginase. As such, arginine has the potential to influence diverse fundamental processes in the lung. Here we report that the arginine transport protein, cationic amino acid transporter (CAT)2, has a critical role in regulating lung inf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothenberg ME,Doepker MP,Lewkowich IP,Chiaramonte MG,Stringer KF,Finkelman FD,MacLeod CL,Ellies LG,Zimmermann N

更新日期：2006-10-03 00:00:00

abstract：:The cycles of carbon and oxygen at the Earth surface are intimately linked, where the burial of organic carbon into sediments represents a source of oxygen to the surface environment. This coupling is typically quantified through the isotope records of organic and inorganic carbon. Yet, the late Neoproterozoic Eon, th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bjerrum CJ,Canfield DE

更新日期：2011-04-05 00:00:00

abstract：:Analysis of the activity and structure of lower vertebrate alcohol dehydrogenases reveals that relationships between the classical liver and yeast enzymes need not be continuous. Both the ethanol activity of class I-type alcohol dehydrogenase (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) and the glutathione-dependent form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Danielsson O,Jörnvall H

更新日期：1992-10-01 00:00:00

abstract：:We have recently established that several mRNAs encode the PR264/SC35 splicing factor and that their expression is related to that of c-Myb in human hematopoietic cells. We now report that the various PR264 mRNAs whose expression is transactivated by c-Myb proteins encode an identical PR264/SC35 polypeptide. These mRN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sureau A,Perbal B

更新日期：1994-02-01 00:00:00

abstract：:Vertebrate embryogenesis and organogenesis are driven by cell biological processes, ranging from mitosis and migration to changes in cell size and polarity, but their control and causal relationships are not fully defined. Here, we use the developing limb skeleton to better define the relationships between mitosis and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Junge JA,Arnesano C,Gross GG,Miner JH,Moats R,Roberts RW,Arnold DB,Fraser SE

更新日期：2018-11-13 00:00:00

abstract：:The spatial coordination of growth is of central importance for the regulation of plant tissue architecture. Individual layers, such as the epidermis, are clonally propagated and structurally maintained by symmetric cell divisions that are oriented along the plane of the layer. The developmental control of this proces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Enugutti B,Kirchhelle C,Oelschner M,Torres Ruiz RA,Schliebner I,Leister D,Schneitz K

更新日期：2012-09-11 00:00:00

abstract：:The apparent detection of an exoplanet orbiting Fomalhaut was announced in 2008. However, subsequent observations of Fomalhaut b raised questions about its status: Unlike other exoplanets, it is bright in the optical and nondetected in the infrared, and its orbit appears to cross the debris ring around the star withou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gáspár A,Rieke GH

更新日期：2020-05-05 00:00:00

abstract：:We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dirks RM,Pierce NA

更新日期：2004-10-26 00:00:00

abstract：:In most animals, longevity is achieved at the expense of fertility, but queen honey bees do not show this tradeoff. Queens are both long-lived and fertile, whereas workers, derived from the same genome, are both relatively short-lived and normally sterile. It has been suggested, on the basis of results from workers, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Corona M,Velarde RA,Remolina S,Moran-Lauter A,Wang Y,Hughes KA,Robinson GE

更新日期：2007-04-24 00:00:00

abstract：:Herpes simplex virus (HSV) is known to bud from the inner membrane of the nuclear envelope. The structural gene for the glycoprotein gB, which is essential for virus entry and cell fusion induced by HSV type 1, has been cloned in a transient expression vector containing the adenovirus major late promoter, tripartite l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ali MA,Butcher M,Ghosh HP

更新日期：1987-08-01 00:00:00