Abstract:
:The first demonstration of a method to incorporate radiolabeled methyl groups from [14C]formaldehyde into membrane proteins in human erythrocytes by reductive methylation in the presence of NaCNBH3 is described. This method is based on the work of Jentoft and Dearborn [(1979) J. Biol. Chem. 254, 4359-4365], who demonstrated the feasibility of this procedure in soluble proteins. The results of the present study suggest that cytoskeletal protein components of erythrocyte membranes are primarily labeled and that this procedure provides a useful method to investigate the structure of membrane proteins.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Butterfield DA,Shahabuddin Mdoi
10.1007/BF01136722subject
Has Abstractpub_date
1982-04-01 00:00:00pages
235-9issue
4eissn
0144-8463issn
1573-4935journal_volume
2pub_type
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journal_title:Bioscience reports
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journal_title:Bioscience reports
pub_type: 杂志文章
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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更新日期:2020-11-27 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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