Reductive methylation of membrane proteins in human erythrocyte ghosts.

Abstract:

:The first demonstration of a method to incorporate radiolabeled methyl groups from [14C]formaldehyde into membrane proteins in human erythrocytes by reductive methylation in the presence of NaCNBH3 is described. This method is based on the work of Jentoft and Dearborn [(1979) J. Biol. Chem. 254, 4359-4365], who demonstrated the feasibility of this procedure in soluble proteins. The results of the present study suggest that cytoskeletal protein components of erythrocyte membranes are primarily labeled and that this procedure provides a useful method to investigate the structure of membrane proteins.

journal_name

Biosci Rep

journal_title

Bioscience reports

authors

Butterfield DA,Shahabuddin M

doi

10.1007/BF01136722

subject

Has Abstract

pub_date

1982-04-01 00:00:00

pages

235-9

issue

4

eissn

0144-8463

issn

1573-4935

journal_volume

2

pub_type

杂志文章
  • Modulation of the extracellular matrix patterning of thrombospondins by actin dynamics and thrombospondin oligomer state.

    abstract::Thrombospondins (TSPs) are evolutionarily-conserved, secreted glycoproteins that interact with cell surfaces and extracellular matrix (ECM) and have complex roles in cell interactions. Unlike the structural components of the ECM that form networks or fibrils, TSPs are deposited into ECM as arrays of nanoscale puncta. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20140168

    authors: Hellewell AL,Gong X,Schärich K,Christofidou ED,Adams JC

    更新日期:2015-05-20 00:00:00

  • Apo, Zn2+-bound and Mn2+-bound structures reveal ligand-binding properties of SitA from the pathogen Staphylococcus pseudintermedius.

    abstract::The Gram-positive bacterium Staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. S. pseudintermedius also causes life-threatening human infections. Furthermore, methicillin-resistant S. pseudintermedius is emerging, resembling the human health threa...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20140088

    authors: Abate F,Malito E,Cozzi R,Lo Surdo P,Maione D,Bottomley MJ

    更新日期:2014-11-24 00:00:00

  • Novel protective effects of pulsed electromagnetic field ischemia/reperfusion injury rats.

    abstract::Extracorporeal pulsed electromagnetic field (PEMF) has shown the ability to regenerate tissue by promoting cell proliferation. In the present study, we investigated for the first time whether PEMF treatment could improve the myocardial ischaemia/reperfusion (I/R) injury and uncovered its underlying mechanisms.In our s...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20160082

    authors: Ma F,Li W,Li X,Tran BH,Suguro R,Guan R,Hou C,Wang H,Zhang A,Zhu Y,Zhu Y

    更新日期:2016-12-05 00:00:00

  • The relationship between the chromophore moiety and the cation binding sites in bacteriorhodopsin.

    abstract::Bleaching of the purple membrane strongly reduces the number of divalent cation binding sites as well as their affinities. Conversely, deionization of the bleached membrane drastically inhibits the chromophore regeneration. Proteolysis experiments using bromelain show that the bleached membrane has an additional cleav...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01114972

    authors: Duñach M,Seigneuret M,Rigaud JL,Padrós E

    更新日期:1986-11-01 00:00:00

  • Inhibitory effect of clonidine upon adenylate cyclase activity, cyclic AMP production, and insulin release in rat pancreatic islets.

    abstract::Conflicting opinions were recently expressed concerning the possible effect of alpha 2-adrenergic agonists upon cyclic AMP production in pancreatic islets. In the present study, clonidine inhibited glucose-induced insulin release from rat pancreatic islets, this inhibitory effect being abolished by idazoxan. Clonidine...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01122227

    authors: Garcia-Morales P,Dufrane SP,Sener A,Valverde I,Malaisse WJ

    更新日期:1984-06-01 00:00:00

  • Comparative analysis of the complete mitochondrial genomes of three rockfishes (Scorpaeniformes, Sebastiscus) and insights into the phylogenetic relationships of Sebastidae.

    abstract::Mitochondrial genome is a powerful molecule marker to provide information for phylogenetic relationships and revealing molecular evolution in ichthyological studies. Sebastiscus species, a marine rockfish, are of essential economic value. However, the taxonomic status and phylogenetic relationships of Sebastidae have ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20203379

    authors: Jia C,Zhang X,Xu S,Yang T,Yanagimoto T,Gao T

    更新日期:2020-12-23 00:00:00

  • Fluorimetric analysis of phospholipase activity in Tetrahymena pyriformis GL.

    abstract::The unicellular Tetrahymena enzymatically split the synthetic phosphodiester, 4-methylumbelliferyl phosphocholine substrate. The enzyme activity was completely blocked in vitro and drastically inhibited in vivo by G-protein activating fluorides (NaF; AIF4- and BeF3-). The phospholipase A2 inhibitor, quinacrine, and th...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1023/a:1020154325630

    authors: Kovács P,Csaba G

    更新日期:1999-04-01 00:00:00

  • Long non-coding RNA SPRY4-IT1 promotes epithelial-mesenchymal transition of cervical cancer by regulating the miR-101-3p/ZEB1 axis.

    abstract:BACKGROUND:Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4 intronic transcript 1 (SPRY4-IT1) predicates poor prognosis and promotes tumor progress in cervical cancer (CC). However, ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20181339

    authors: Fan MJ,Zou YH,He PJ,Zhang S,Sun XM,Li CZ

    更新日期:2019-06-04 00:00:00

  • Investigations on myelination in vitro: thyroid hormone receptors in cultures of cells dissociated from embryonic mouse brain.

    abstract::Brain cells dissociated from 15-day-old embryonic mice and grown in culture contain both cytosolic and nuclear receptors for L-3,5,3'-triiodothyronine (L-T3). KD values for L-T3 of 3.05 X 10(-9) M and 4.2 X 10(-9) M were determined with the cytosolic and nuclear receptors respectively. These cultured cells, which are ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01114868

    authors: Shanker G,Bhat NR,Pieringer RA

    更新日期:1981-04-01 00:00:00

  • Influence of haem environment on the catalytic properties of the tetrathionate reductase TsdA from Campylobacter jejuni.

    abstract::Bifunctional dihaem cytochrome c thiosulfate dehydrogenases/tetrathionate reductases (TsdA) exhibit different catalytic properties depending on the source organism. In the human food-borne intestinal pathogen Campylobacter jejuni, TsdA functions as a tetrathionate reductase enabling respiration with tetrathionate as a...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20160457

    authors: Kurth JM,Butt JN,Kelly DJ,Dahl C

    更新日期:2016-12-09 00:00:00

  • Diagnosis of nonsense mutations in Aspergillus nidulans.

    abstract::Three genotypically suppressible alleles, a1X4, alcA125, and niaD500, are phenotypically suppressed by aminoglycoside antibiotics. Unsuppressible alleles at these loci are unaffected as are known missense mutations at the yA and gdhA loci. This is consistent with the premise that the suppressible mutations are nonsens...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01121566

    authors: Martinelli SD,Roberts TJ

    更新日期:1983-01-01 00:00:00

  • The regulation roles of miR-125b, miR-221 and miR-27b in porcine Salmonella infection signalling pathway.

    abstract::miRNAs are non-coding RNA molecules typically 18-22 nucleotides long that can suppress the expression of their target genes. Several laboratories have attempted to identify miRNAs from the pig that are involved in Salmonella infection. These bioinformatics strategies using the newly available genomic sequence are gene...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20160243

    authors: Yao M,Gao W,Yang J,Liang X,Luo J,Huang T

    更新日期:2016-08-31 00:00:00

  • LncRNA LINC00668 promotes cell proliferation, migration, invasion ability and EMT process in hepatocellular carcinoma by targeting miR-532-5p/YY1 axis.

    abstract::Liver cancer is now one of the most lethal and commonest cancers in the world, among which over 90% is hepatocellular carcinoma (HCC). Recent studies have confirmed long non-coding RNAs (lncRNAs) are implicated in carcinogenesis. It has been reported lncRNA LINC00668 serves as an oncogene in several cancers. However, ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20192697

    authors: Xuan W,Zhou C,You G

    更新日期:2020-05-29 00:00:00

  • Increased stability and catalytic efficiency of yeast hexokinase upon interaction with zwitterionic micelles. Kinetics and conformational studies.

    abstract::The effect of ligands (glucose, ATP and Mg2+) and zwitterionic micelles of lysophosphatidylcholine (LPC) or N-hexadecyl-N,N-dimethyl-3-ammonium propanesulfonate (HPS) in the yeast hexokinase (HK) stability was studied at 35 degrees C. The thermal inactivation kinetics followed one-exponential decay. The effect of liga...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1023/a:1005583117296

    authors: Guerra R,Bianconi ML

    更新日期:2000-02-01 00:00:00

  • The pathogenic role of connective tissue growth factor in osteoarthritis.

    abstract::Osteoarthritis (OA) is the most common form of arthritis, and connective tissue growth factor (CTGF) is found to be up-regulated in adjacent areas of cartilage surface damage. CTGF is present in osteophytes of late stage OA. In the present study, we have reviewed association of CTGF in the development and progression ...

    journal_title:Bioscience reports

    pub_type: 杂志文章,评审

    doi:10.1042/BSR20191374

    authors: Tu M,Yao Y,Qiao FH,Wang L

    更新日期:2019-07-18 00:00:00

  • Potential role of miR-155-5p in fat deposition and skeletal muscle development of chicken.

    abstract::miR-155 has multiple functions in many physiological and pathological processes. However, little is known about the expression characteristics of avian miR-155. In the present study, partial pri-miR-155 sequences were cloned from AA+ broiler, Sanhuang broiler and Hy-Line Brown layer, respectively. Stem-loop qRT-PCR wa...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20193796

    authors: Xu S,Chang Y,Wu G,Zhang W,Man C

    更新日期:2020-06-26 00:00:00

  • Zinc lozenges: cold cure or candy? Solution chemistry determinations.

    abstract::Common colds were shortened by 7 days in a 1984 clinical trial using zinc gluconate throat lozenges each 2 h. Between then and 2004, 10 other double-blind, placebo-controlled clinical trials showed widely varying results. This re-analysis of these trials presents solution chemistry methods to elucidate differences in ...

    journal_title:Bioscience reports

    pub_type: 杂志文章,meta分析

    doi:10.1023/b:bire.0000037754.71063.41

    authors: Eby GA

    更新日期:2004-02-01 00:00:00

  • Genetic and structural validation of Aspergillus fumigatus N-acetylphosphoglucosamine mutase as an antifungal target.

    abstract::Aspergillus fumigatus is the causative agent of IA (invasive aspergillosis) in immunocompromised patients. It possesses a cell wall composed of chitin, glucan and galactomannan, polymeric carbohydrates synthesized by processive glycosyltransferases from intracellular sugar nucleotide donors. Here we demonstrate that A...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20130053

    authors: Fang W,Du T,Raimi OG,Hurtado-Guerrero R,Mariño K,Ibrahim AF,Albarbarawi O,Ferguson MA,Jin C,Van Aalten DM

    更新日期:2013-09-04 00:00:00

  • Participation of chloroplasts in plant apoptosis.

    abstract::Mitochondria are known to participate in the initiation of programmed cell death (PCD) in animals and in plants. The role of chloroplasts in PCD is still unknown. We describe a new system to study PCD in plants; namely, leaf epidermal peels. The peel represents a monolayer consisting of cells of two types: phototrophi...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1023/a:1025576307912

    authors: Samuilov VD,Lagunova EM,Kiselevsky DB,Dzyubinskaya EV,Makarova YV,Gusev MV

    更新日期:2003-04-01 00:00:00

  • Association between epicardial adipose tissue and adverse outcomes in coronary heart disease patients with percutaneous coronary intervention.

    abstract::We assessed the relationship between the volume of epicardial adipose tissue and long-term outcomes in patients with coronary heart disease (CHD) undergoing percutaneous coronary intervention (PCI). The patients with CHD were followed for at least 2 years after PCI. The epicardial adipose tissue volume (EATV) was meas...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20182278

    authors: Lu C,Jia H,Wang Z

    更新日期:2019-05-07 00:00:00

  • Development and validation of nomogram to predict risk of survival in patients with laryngeal squamous cell carcinoma.

    abstract::To the best of our knowledge, this is the first study established a nomogram to predict survival probability in Asian patients with LSCC. A risk prediction nomogram for patients with LSCC, incorporating easily assessable clinicopathologic factors, generates more precise estimations of the survival probability when com...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20200228

    authors: Cui J,Wang L,Zhong W,Chen Z,Tan X,Yang H,Chen J,Liu G

    更新日期:2020-08-28 00:00:00

  • Determination of the internal volume of reconstituted sendai virus envelopes by quenching of calcein fluorescence.

    abstract::Sendai virus envelopes (SVE) were isolated from Sendai virus particles by Triton X-100 solubilization and ultracentrifugation. The envelopes were reconstituted in the presence of the fluorescent dye calcein by gradual removal of the detergent with Bio-beads SM-2. The internal volume of reconstituted Sendai virus envel...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01121911

    authors: Bartzatt RL,Volsky DJ

    更新日期:1984-07-01 00:00:00

  • Calpain-2 participates in the process of calpain-1 inactivation.

    abstract::Calpain-1 and calpain-2 are highly structurally similar isoforms of calpain. The calpains, a family of intracellular cysteine proteases, cleave their substrates at specific sites, thus modifying their properties such as function or activity. These isoforms have long been considered to function in a redundant or comple...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20200552

    authors: Shinkai-Ouchi F,Shindo M,Doi N,Hata S,Ono Y

    更新日期:2020-11-27 00:00:00

  • miR-152/TNS1 axis inhibits non-small cell lung cancer progression through Akt/mTOR/RhoA pathway.

    abstract:AIM:The purpose of the present study was to explore the function and mechanism of tensin 1 (TNS1) in non-small cell lung cancer (NSCLC) progression. METHODS:The expression of TNS1 in NSCLC cells and tissues was assessed by RT-PCR and Western blot. Besides, Kaplan-Meier survival analysis was recruited to explore the as...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20201539

    authors: Duan J,Wang L,Shang L,Yang S,Wu H,Huang Y,Miao Y

    更新日期:2021-01-29 00:00:00

  • Identification of key genes involved in type 2 diabetic islet dysfunction: a bioinformatics study.

    abstract::Aims: To identify the key differentially expressed genes (DEGs) in islet and investigate their potential pathway in the molecular process of type 2 diabetes.Methods: Gene Expression Omnibus (GEO) datasets (GSE20966, GSE25724, GSE38642) of type 2 diabetes patients and normal controls were downloaded from GEO database. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20182172

    authors: Zhong M,Wu Y,Ou W,Huang L,Yang L

    更新日期:2019-05-31 00:00:00

  • Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-κB pathway.

    abstract:OBJECTIVE:Our aim was to investigate the effect of emodin on intestinal and lung injury induced by acute intestinal injury in rats and explore potential molecular mechanisms. METHODS:Healthy male Sprague-Dawley (SD) rats were randomly divided into five groups (n=10, each group): normal group; saline group; acute intes...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20201605

    authors: Qian J,Li G,Jin X,Ma C,Cai W,Jiang N,Zheng J

    更新日期:2020-09-30 00:00:00

  • Ambra1 induces autophagy and desensitizes human prostate cancer cells to cisplatin.

    abstract::Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autoph...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20170770

    authors: Liu J,Chen Z,Guo J,Wang L,Liu X

    更新日期:2019-08-23 00:00:00

  • C1r serine proteinase of human complement: a case of intramolecular autolytic activation.

    abstract::This paper presents a short review of our contribution to the knowledge of the structure and function of human C1r, the activation unit of C1, the first component of the classical pathway of complement. On the basis of the domain structure of C1r, a model accounting for its autolytic activation mechanism is proposed. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01119894

    authors: Arlaud GJ,Colomb MG,Villiers CL

    更新日期:1985-10-01 00:00:00

  • Identification of Ly2 members as antimicrobial peptides from zebrafish Danio rerio.

    abstract::The emergence of multidrug-resistant (MDR) microbes caused by overuse of antibiotics leads to urgent demands for novel antibiotics exploration. Our recent data showed that Ly2.1-3 (a novel lymphocyte antigen 6 (Ly6) gene cluster) were proteins with cationic nature and rich in cysteine content, that are characteristic ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20160265

    authors: Liu X,Cao X,Wang S,Ji G,Zhang S,Li H

    更新日期:2017-01-17 00:00:00

  • Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse.

    abstract::FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural simila...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20194431

    authors: Weiss AKH,Naschberger A,Cappuccio E,Metzger C,Mottes L,Holzknecht M,von Velsen J,Bowler MW,Rupp B,Jansen-Dürr P

    更新日期:2020-03-27 00:00:00