Abstract：
:The unicellular Tetrahymena enzymatically split the synthetic phosphodiester, 4-methylumbelliferyl phosphocholine substrate. The enzyme activity was completely blocked in vitro and drastically inhibited in vivo by G-protein activating fluorides (NaF; AIF4- and BeF3-). The phospholipase A2 inhibitor, quinacrine, and the protein phosphatase inhibitor, neomycin, inhibited the enzyme activity in vitro and activated it in vivo. Another phospholipase A2 inhibitor 4-bromo phenacyl bromide was ineffective in vivo and in vitro alike, as well as the cyclooxygenase inhibitor indomethacin. Results of these experiments indicate that some treatments could be specific for a well defined activity (e.g., phospholipase A2, G-protein) but subject to influence by other enzymes (e.g., phospholipase C, sphingomyelinase). The experiments call attention to the differences in the results of the in vivo and in vitro studies.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Kovács P,Csaba Gdoi
10.1023/a:1020154325630keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
81-7issue
2eissn
0144-8463issn
1573-4935journal_volume
19pub_type
杂志文章abstract：:An antibody was raised to the synthetic pentapeptide pGluHisProGlyLys which, in radioimmunoassay (RIA), could detect the pentapeptide at a level of 10 fmole per tube and exhibited less than 0.5 per cent cross reactivity with a series of related peptides. The RIA was used to demonstrate the presence of C-terminally ext...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114948
更新日期：1986-06-01 00:00:00
abstract：:The present study investigated the effects of Isorhamnetin on two types of prostate cancer cells (androgen-independent and androgen-dependent) and explored its possible mechanisms underlying such effects. Treatment with Isorhamnetin significantly inhibited cell growth and induced lactate dehydrogenase (LDH) release of...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20192826
更新日期：2020-03-27 00:00:00
abstract：:Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1010425932561
更新日期：2001-02-01 00:00:00
abstract：:Gallbladder cancer (GBC) is a relatively rare but fatal gastrointestinal tumor. The microRNA-33b (miR-33b), a member of miR-33 family, is reported to function as a tumor suppressor in various cancers. Notably, miR-33 was predicted to target CROCC based on microarray-based analysis. Hereby, we aimed to characterize the...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190108
更新日期：2020-01-31 00:00:00
abstract：:As a zinc transporter, SLC39A7 (zip7) is vital in intestinal epithelial self-renewal, and recent studies suggested that SLC39A7 was related to cancer progression. Whereas, little is known about the role of SLC39A7 in gastric cancer (GC). In the present study, qRT-PCR analysis demonstrated that SLC39A7 mRNA level was i...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200041
更新日期：2020-02-28 00:00:00
abstract：:Peri-implantitis, which is characterized by dense inflammatory infiltrates and increased osteoclast activity, can lead to alveolar bone destruction and implantation failure. miRNAs participate in the regulation of various inflammatory diseases, such as periodontitis and osteoporosis. Therefore, the present study aimed...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170768
更新日期：2017-10-11 00:00:00
abstract：:Kawasaki disease (KD) is a type of disease that includes the development of a fever that lasts at least 5 days and involves the clinical manifestation of multicellular vasculitis. KD has become one of the most common pediatric cardiovascular diseases. Previous studies have reported that miR-218 rs11134527 A>G is assoc...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20180367
更新日期：2018-05-22 00:00:00
abstract：:Poly(A)+RNA was isolated from brown adipose tissue of cold acclimated rats and a fraction enriched for uncoupling protein mRNA was used to generate a cDNA library in pBR 322. Immunological screening of 1,500 colonies with an affinity-purified antiserum against the uncoupling protein yielded five positive clones, pUCPr...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01145183
更新日期：1986-01-01 00:00:00
abstract：:Acidic airway microenvironment is one of the representative pathophysiological features of chronic inflammatory respiratory diseases. Epithelial barrier function is maintained by TJs (tight junctions), which act as the first physical barrier against the inhaled substances and pathogens of airway. As previous studies d...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20130087
更新日期：2013-10-31 00:00:00
abstract：:The neurotransmitter, serotonin has emerged as a tumor growth factor and immune response regulator through complex signaling pathways. Yip1 Interacting Factor Homolog B (YIF1B) is a membrane protein involved in serotonin receptor (HTR) membrane trafficking and signal transmission in neuropathy. Participation of YIF1B ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201384
更新日期：2020-07-31 00:00:00
abstract：:This paper presents a short review of our contribution to the knowledge of the structure and function of human C1r, the activation unit of C1, the first component of the classical pathway of complement. On the basis of the domain structure of C1r, a model accounting for its autolytic activation mechanism is proposed. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119894
更新日期：1985-10-01 00:00:00
abstract：:The cDNA encoding about half of an antigenic non-surface schistosome parasite protein of Mr 97 K has recently been cloned and sequenced (Lanar, Pearce, James and Sher (1986) Science 234:593-596). Analysis of this sequence, together with the properties of the native protein, reveals that this protein is paramyosin, the...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122722
更新日期：1987-01-01 00:00:00
abstract：:Thrombospondin-2 (THBS2) is a secreted protein overexpressed in numerous cancers and may function as a diagnostic tumor marker. The objective of the present study was to investigate the diagnostic performance of serum THBS2 in early stage non-small-cell lung cancer (NSCLC). Serum THBS2 and Cyfra21-1 level were evaluat...
journal_title：Bioscience reports
pub_type： 临床试验,杂志文章
doi：10.1042/BSR20190476
更新日期：2019-07-25 00:00:00
abstract：:Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autoph...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170770
更新日期：2019-08-23 00:00:00
abstract：BACKGROUND:Breast cancer (BC) is the most common malignancy among females worldwide. The tumor microenvironment usually prevents effective lymphocyte activation and infiltration, and suppresses infiltrating effector cells, leading to a failure of the host to reject the tumor. CC chemokines play a significant role in in...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20202042
更新日期：2020-11-27 00:00:00
abstract：:The identification of the sodium potassium pump as a Na+, K+ -ATPase is described. ...
journal_title：Bioscience reports
pub_type： 传,历史文章
doi：10.1023/a:1020196612909
更新日期：1998-08-01 00:00:00
abstract：:Maclurin, the potent non-specific blood-group hemagglutinin present in extracts of Maclura pomifera, has been purified by a new biospecific affinity-chromatographic procedure. Additional studies have indicated that this hemagglutinin occurs as five closely related tetrameric protein isoforms derived from two non-coval...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01136724
更新日期：1982-04-01 00:00:00
abstract：:Canstatin, the non-collagenous domain of collagen type IV α-chains, belongs to a series of collagen-derived angiogenic inhibitors. In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. The volume and weight of tumours in mice treated wit...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20140012
更新日期：2014-04-01 00:00:00
abstract：:Kinesin family member 14 (KIF14) is a member of kinesin family proteins which have been found to be dysregulated in various cancer types. However, the expression of KIF14 and its potential prognostic significance have not been investigated in cervical cancer. Real-time PCR was performed to assess the expression levels...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20150314
更新日期：2016-04-05 00:00:00
abstract：:A family of artificial proteins, named αRep, based on a natural family of helical repeat was previously designed. αRep members are efficiently expressed, folded and extremely stable proteins. A large αRep library was constructed creating proteins with a randomized interaction surface. In the present study, we show tha...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20150080
更新日期：2015-06-12 00:00:00
abstract：:Liver cancer is now one of the most lethal and commonest cancers in the world, among which over 90% is hepatocellular carcinoma (HCC). Recent studies have confirmed long non-coding RNAs (lncRNAs) are implicated in carcinogenesis. It has been reported lncRNA LINC00668 serves as an oncogene in several cancers. However, ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20192697
更新日期：2020-05-29 00:00:00
abstract：:A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubilized proteins bind specifically [125I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of deter...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01145177
更新日期：1986-01-01 00:00:00
abstract：:PDC (pyruvate dehydrogenase complex) is a multi-enzyme complex comprising an E1 (pyruvate decarboxylase), an E2 (dihydrolipomide acetyltransferase) and an E3 (dihydrolipoamide dehydrogenase). PDC catalyses the decarboxylation of pyruvate and forms acetyl-CoA and NADH. In the human malaria parasite Plasmodium falciparu...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20140150
更新日期：2015-01-14 00:00:00
abstract：:Ehrlich Ascites Tumour cells were irradiated with UV-light to crosslink DNA to proteins in vivo. The DNA fragments associated with the nuclear lamina were purified and characterized. The results of the Cot analysis and the hybridization experiments suggest that the DNA fragments attached to the nuclear lamina although...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119802
更新日期：1989-10-01 00:00:00
abstract：:Merremia vitifolia (Burm.f.) Hallier f., an ethnomedicinally important plant, used in the tribal areas to treat various ailments including fever, headache, eye inflammation, rheumatism, dysentery, jaundice and urinary diseases. The present study explored the biological efficacy of the aqueous fraction of M. vitifolia ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20203022
更新日期：2021-01-29 00:00:00
abstract：:Recent developments in the study of peptide amidation are reviewed. The main areas covered are assay procedures, purification of amidating enzymes, co-factors and regulation, mechanism and specificity of the amidating reaction, and multiple forms of the amidating enzyme and glycosylation. Discussion is presented on as...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1007/BF01122123
更新日期：1987-12-01 00:00:00
abstract：:For improving Ostwald's viscometry, which is time-consuming and requires a relatively large volume of specimen to determine viscosity, we developed a capillary microviscometric method with an appropriate mathematical model, and have compared this method with Ostwald's method. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01121952
更新日期：1983-02-01 00:00:00
abstract：:Cholera toxin (CT) is composed of a disulfide-linked A1/A2 heterodimer and a ring-like, cell-binding B homopentamer. The catalytic A1 subunit must dissociate from CTA2/CTB5 to manifest its cellular activity. Reduction of the A1/A2 disulfide bond is required for holotoxin disassembly, but reduced CTA1 does not spontane...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181320
更新日期：2018-09-07 00:00:00
abstract：:Secretion of high molecular weight (HMW) adiponectin is dependent on post-translational modification (PTM) of conserved lysines in the collagenous domain. The present study aims to characterize the enzymes responsible for the PTM of conserved lysines which leads to HMW adiponectin secretion, and to define its signific...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170105
更新日期：2017-05-17 00:00:00
abstract：:When the amino-acid sequence of the 108-residue, rat tumour calcium-binding protein, oncomodulin, was aligned with that of rat muscle parvalbumin, 55 homologous positions were found, with an additional 33 single base-pair substitutions. This extensive homology, with virtual identity of the calcium-binding domains, sig...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01121034
更新日期：1983-11-01 00:00:00
