Vasoactive intestinal peptide (VIP) binding to solubilized material from rat liver plasma membranes.

Abstract:

:A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubilized proteins bind specifically [125I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of detergent (below 0.001%) and is inhibited by native peptide. A molecular weight of about 80,000 was estimated for VIP-binding proteins by reference to a series of globular markers of proteins. Binding to VIP soluble proteins is specific and dependent on time as studied by the Hummel and Dreyer (Biochim. Biophys. Acta 63:530-532, 1962) assay.

journal_name

Biosci Rep

journal_title

Bioscience reports

authors

Guerrero JM,Calvo JR,Garrido MR,Molinero P,Osuna C,Jiménez J,Goberna R

doi

10.1007/BF01145177

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

39-44

issue

1

eissn

0144-8463

issn

1573-4935

journal_volume

6

pub_type

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