The bovine lymphoid system. III. A monoclonal antibody specific for bovine cell surface and serum IgM.

Abstract:

:Mouse spleen cells from animals immunized with bovine peripheral blood lymphocytes were fused to X63 . Ag8 myeloma cells and the activity of one of the resulting myeloma hybrids was characterized. The product of this clone (B5/4.1.4) binds to pentameric bovine IgM isolated from serum but not to serum IgG1 or IgG2. This reagent also binds to cell surface (monomeric) IgM and can be used in immunofluorescence assays to enumerate IgM-bearing cells in lymphoid cell suspensions and to examine B lymphocytes or B lymphocyte derived cells in tissue sections.

journal_name

Immunology

journal_title

Immunology

authors

Pinder M,Musoke AJ,Morrison WI,Roelants GE

subject

Has Abstract

pub_date

1980-07-01 00:00:00

pages

359-65

issue

3

eissn

0019-2805

issn

1365-2567

journal_volume

40

pub_type

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