Abstract：
:A fully humanized anti-CD4 antibody was studied for its effects on resting and activated CD4 T cells. Whereas the antibody was poorly lytic, it induced dramatic down-modulation of CD4 expression on both types of cell. In order to down-modulate CD4 on resting, normal CD4 T cells there was an absolute requirement for FcR-mediated cross-linking of the anti-CD4 antibody, and only CD4 levels were affected. When activated cloned T-cell lines were studied there was no requirement for cross-linking and several other cell surface markers were also affected. Although the total cellular CD4 was reduced in the down-modulated cells, as judged by Western blot analysis, that CD4 which remained was associated with p56lck. The results are discussed in relation to the potential use of humanized anti-CD4 antibodies in the therapy of autoimmune disease and the choice of antibody isotype for such a therapeutic antibody.
journal_name
Immunologyjournal_title
Immunologyauthors
Bartholomew M,Brett S,Barber K,Rossman C,Crowe S,Tite Jsubject
Has Abstractpub_date
1995-05-01 00:00:00pages
41-8issue
1eissn
0019-2805issn
1365-2567journal_volume
85pub_type
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