Identification by high-performance liquid chromatography of immunoreactive substance P released from isolated rat spinal cord.

Abstract:

:An attempt was made to identify the immunoreactive substance P (SP) released from isolated rat spinal cords using reversed-phase high-performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA) for SP. Soaking the spinal cords of newborn rats in Krebs solution containing 90 mM K+ evoked a release of immunoreactive SP as well as of GABA and glycine in a calcium-dependent manner. Capsaicin also evoked a release of immunoreactive SP but not of GABA and glycine. The immunoreactive SP released from rat spinal cords by high K+ or capsaicin was analyzed by HPLC. A single peak was detected by RIA whose elution position coincided with that of synthetic SP.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Akagi H,Otsuka M,Yanagisawa M

doi

10.1016/0304-3940(80)90157-3

subject

Has Abstract

pub_date

1980-12-01 00:00:00

pages

259-63

issue

3

eissn

0304-3940

issn

1872-7972

pii

0304-3940(80)90157-3

journal_volume

20

pub_type

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