Biosynthesis of MSH and related peptides in the pars intermedia of the mouse: a pulse-chase analysis.

abstract：:Procedures have been developed which provide simple means of determining binding constants of steroid receptors for glucocorticoids in mouse lymphoid cell lines and of characterizing the interaction of the steroid--receptor complex with the nucleus. An average of 70% of the steroid--receptor complexes is found associa...

journal_title：Molecular and cellular endocrinology

authors： Pfahl M,Sandros T,Bourgeois S

更新日期：1978-04-01 00:00:00

abstract：:The teleost brain is characterized by exceptionally high levels of aromatase, the enzyme that converts androgens into estrogens, and by its continuous growth throughout life. Gonadal estrogens have been implicated in sex differentiation and the control of reproduction in adult fish, but the role of neural estrogens du...

journal_title：Molecular and cellular endocrinology

authors： Blázquez M,Piferrer F

更新日期：2004-04-30 00:00:00

abstract：:The involvement of cyclic-AMP (cAMP) as a potential second messenger in the neurohormonal control of ovarian steroidogenesis was investigated in the adult female blowfly Phormia regina. Individual measurements of ovarian cAMP concentrations and of ovarian biosynthesis of ecdysteroids, stimulated after a protein meal, ...

journal_title：Molecular and cellular endocrinology

authors： Manière G,Vanhems E,Delbecque J

更新日期：2000-10-25 00:00:00

abstract：:The adrenal cortex functions to produce steroid hormones necessary for life. To maintain its functional capacity throughout life, the adrenal cortex must be continually replenished and rapidly repaired following injury. Moreover, the adrenal responds to endocrine-mediated organismal needs, which are highly dynamic and...

journal_title：Molecular and cellular endocrinology

authors： Hammer GD,Basham KJ

更新日期：2021-01-01 00:00:00

abstract：:The capability of rat pituitary cells to express receptors for pituitary adenylate cyclase activating polypeptide (PACAP) and VIP was evaluated by binding studies and measurement of adenylate cyclase activity on whole gland preparations and by reverse transcriptase-polymerase chain reaction (TR-PCR) using specific pri...

journal_title：Molecular and cellular endocrinology

authors： Vertongen P,Velkeniers B,Hooghe-Peters E,Robberecht P

更新日期：1995-09-22 00:00:00

abstract：:Accumulating evidence suggests that the human follitropin receptor is unusually sensitive to mutation. Previous results (Mol. Cell. Endo. 166 (2000) 101) determined that scanning mutations in a.a. 12-14 and 22-30 neither bound follitropin nor were present on the cell surface, suggesting that these regions are involved...

journal_title：Molecular and cellular endocrinology

authors： Nechamen CA,Dias JA

更新日期：2003-03-28 00:00:00

abstract：:Adrenocortical tumors (ACTs) frequently cause steroid excess and present cell-cycle dysregulation. cAMP/PKA signaling is involved in steroid synthesis and play a role in cell-cycle regulation. We investigated, by cell synchronization in the different phases of the cell-cycle, the control of steroidogenesis and the con...

journal_title：Molecular and cellular endocrinology

authors： Rizk-Rabin M,Chaoui-Ibadioune S,Vaczlavik A,Ribes C,Polak M,Ragazzon B,Bertherat J

更新日期：2020-01-15 00:00:00

abstract：:When measured in vitro using human placental microsomal preparations, the aromatization of 16alpha-hydroxytestosterone to estriol and androstenedione to estrone and estradiol proceeds at almost identical initial rates. Important differences between 16alpha-hydroxytestosterone and adrostenedione aromatization are evide...

journal_title：Molecular and cellular endocrinology

authors： Canick JA,Ryan KJ

更新日期：1976-12-01 00:00:00

abstract：:The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, a...

journal_title：Molecular and cellular endocrinology

authors： de Souza BM,Rodrigues M,de Oliveira FS,da Silva LPA,Bouças AP,Portinho CP,Dos Santos BP,Camassola M,Rocha D,Lysakowski S,Martini J,Leitão CB,Nardi NB,Bauer AC,Crispim D

更新日期：2020-04-05 00:00:00

abstract：:Oxytocin receptors (OT-R) are known to be involved in the course of labor since a massive increase in OT-binding sites is observed in the uterus just before parturition. Vasopressin (AVP)-binding sites have also been observed and have been shown to mediate uterotonic responses. To determine exactly which subtypes of O...

journal_title：Molecular and cellular endocrinology

authors： Clerget MS,Elalouf JM,Germain G

更新日期：1997-12-31 00:00:00

abstract：:Chronic restraint stress (CRS) magnifies restraint-induced corticosterone secretion through a mechanism involving increased adrenocortical 5-HT content and turnover. We analysed the impact of CRS on serotonin transporter (SERT) expression and distribution in rat adrenal glands. Male Wistar rats were submitted to CRS (...

journal_title：Molecular and cellular endocrinology

authors： Shanker S,Saroj N,Cordova EJ,Jarillo-Luna RA,López-Sánchez P,Terrón JA

更新日期：2020-12-01 00:00:00

abstract：:The mechanism by which hCG increases the rat testicular vascular permeability was studied by injecting the testes with hCG (0.1-100 ng) together with testicular interstitial fluid (150 mul) or with the fluid alone (control) and measuring the uptake of i.v. injected [125I]hCG and the interstitial fluid volume in the te...

journal_title：Molecular and cellular endocrinology

authors： Veijola M,Rajaniemi H

更新日期：1986-05-01 00:00:00

abstract：:The transforming growth factor beta (TGF-beta) superfamily includes several closely related peptides including the activins and inhibins. Since we recently reported that TGF-beta 1 and beta 2 are potent inducers of steroid 5 alpha-reductase (5 alpha R), we have now studied the effects of these other peptides using pri...

journal_title：Molecular and cellular endocrinology

authors： Antonipillai I,Wahe M,Yamamoto J,Horton R

更新日期：1995-01-01 00:00:00

abstract：:The present study examined the influence of the calcium ionophores A23187 and X537A on the calcitonin (CT) secretory process. The isolated perfused porcine thyroid was used to evaluate ionophore effects on CT secretion and thyroid slices were used to measure 45Ca uptake. Both A23187 and X537A enhanced the rate of CT r...

journal_title：Molecular and cellular endocrinology

authors： Pento JT

更新日期：1986-04-01 00:00:00

abstract：:The adenosine 3', 5'-cyclic monophosphate (cyclic AMP)-dependent protein phosphokinase of rat interstitial cells was characterized by ion-exchange chromatography and sucrose density gradient centrifugation. The 0.2 M NaCl fraction from DEAE-Sephadex showed a small 2.9-S peak of basal enzyme activity, and a large 6.5-S...

journal_title：Molecular and cellular endocrinology

authors： Podesta EJ,Dufau ML,Catt KJ

更新日期：1976-06-01 00:00:00

abstract：:The mechanisms of cytokine-induced beta-cell death are poorly characterised. In rat insulin-producing RINm5F cells, the combination of interleukin-1beta, interferon-gamma and tumour necrosis factor-alpha presently induced disruption of the mitochondrial membrane potential (Deltapsi(m)) as demonstrated by reduced JC-1 ...

journal_title：Molecular and cellular endocrinology

authors： Barbu A,Welsh N,Saldeen J

更新日期：2002-04-25 00:00:00

abstract：:The mechanisms that control the onset of puberty remain within the purview of the neurobiologists who first recognised the concept of removal of a restraint factor to permit reawakening of puberty. The discovery of a ligand-activated G protein receptor-signalling pathway upstream of the GnRH pulse generator adds furth...

journal_title：Molecular and cellular endocrinology

authors： Hughes IA,Kumanan M

更新日期：2006-07-25 00:00:00

abstract：:Poly(A+)-containing mRNA from human term placenta was used to direct protein synthesis in a nuclease-treated rabbit reticulocyte lysate, which is dependent on mRNA and tRNA for maximal activity. The major protein product was human pre-placental lactogen (hPL). Addition of tRNA from rabbit liver, rabbit reticulocyte, h...

journal_title：Molecular and cellular endocrinology

authors： Kelly SJ,Loria J,Gyves MT,Ilan J

更新日期：1983-02-01 00:00:00

abstract：:We have shown previously that activin A increases the number of immunoreactive follicle-stimulating hormone (FSH) cells. To further investigate the action of activin A, we examined its effects on anterior pituitary cells fractionated by centrifugal elutriation. Before activin A treatment, FSH cells were widely distrib...

journal_title：Molecular and cellular endocrinology

authors： Katayama T,Shiota K,Takahashi M

更新日期：1991-05-01 00:00:00

abstract：:Mutations in FGFR1, GNRHR, PROK2, PROKR2, TAC3, or TACR3 underlie isolated hypogonadotropic hypogonadism (IHH) with clinically variable phenotypes, and, by causing incomplete intrauterine activation of the hypothalamic-pituitary-gonadal axis, may lead to cryptorchidism. To investigate the role of defects in these gene...

journal_title：Molecular and cellular endocrinology

authors： Laitinen EM,Tommiska J,Virtanen HE,Oehlandt H,Koivu R,Vaaralahti K,Toppari J,Raivio T

更新日期：2011-07-20 00:00:00

abstract：:The human growth hormone/human chorionic somatomammotropin (hGH/hCS) gene cluster contains five genes: hGH-N, hGH-V, hCS-B, and hCS-L. In this study, the nature of splicing products of their primary transcripts (except hCH-L) was analyzed by nuclease mapping as well as by reverse transcription-polymerase chain reactio...

journal_title：Molecular and cellular endocrinology

authors： Palmetshofer A,Zechner D,Luger TA,Barta A

更新日期：1995-09-22 00:00:00

abstract：:Factors that regulate the tissue specific and developmental expression of the GLUT4 gene, whose transcribed protein is primarily responsible for mediating insulin stimulated glucose transport, are poorly defined. In this study we examined the effects of retinoic acid, a circulating factor that can promote cellular dif...

journal_title：Molecular and cellular endocrinology

authors： Sleeman MW,Zhou H,Rogers S,Ng KW,Best JD

更新日期：1995-02-27 00:00:00

abstract：:Some environmental contaminants and pharmaceuticals increase the incidence of uterine tumors in toxicological studies with rats. These tumors can result from a hormonal imbalance due to rat-specific disrupted pituitary prolactin regulation, and are therefore of questionable relevance for humans. In this study we compa...

journal_title：Molecular and cellular endocrinology

authors： van den Brand AD,Rubinstein E,van den Berg M,van Duursen MBM

更新日期：2019-10-01 00:00:00

abstract：:A thyroid-specific enhancer element located upstream from the bovine thyroglobulin gene had been shown to contain three contiguous regions that are protected by thyroid transcription factor 1 (TTF-1) in footprinting experiments in vitro. The functional relevance of the individual TTF-1 binding sites was investigated i...

journal_title：Molecular and cellular endocrinology

authors： Christophe-Hobertus C,Christophe D

更新日期：1999-03-25 00:00:00

abstract：:Receptor-mediated Ca2+ entry was investigated in fura-2-loaded thyroid FRTL-5 cells. Activation of protein kinase C (PKC) by phorbol myristate acetate (PMA) attenuated the ATP-induced increase in intracellular free Ca2+ ([Ca2+]i). In PKC down-regulated cells, the ATP-induced increase in [Ca2+]i was increased compared ...

journal_title：Molecular and cellular endocrinology

authors： Törnquist K

更新日期：1993-05-01 00:00:00

abstract：:The Polycystic Ovary Syndrome (PCOS) is a complex endocrine disorder characterised both by reproductive and metabolic disturbance, and is the most common cause globally of ovarian infertility. It is also a familial polygenic condition, linked genetically to both Type 2 diabetes and the metabolic syndrome. The striking...

journal_title：Molecular and cellular endocrinology

authors： Corbett S,Morin-Papunen L

更新日期：2013-07-05 00:00:00

abstract：:Medullary thyroid carcinoma (MTC) is a rare neuroendocrine neoplasm of the parafollicular thyroid C cells. Although somatostatin receptors are expressed by MTCs, treatment with octreotide has shown poor efficacy, whereas recently pasireotide has demonstrated antiproliferative effects in persistent postoperative MTCs. ...

journal_title：Molecular and cellular endocrinology

authors： Giardino E,Catalano R,Mangili F,Barbieri AM,Treppiedi D,Elli FM,Dolci A,Contarino A,Spada A,Arosio M,Mantovani G,Peverelli E

更新日期：2021-01-15 00:00:00

abstract：:Classical morphological studies of the folliculo-stellate (FS) cells of the anterior pituitary have suggested that these cells play roles as supporting cells, in metabolism and in macromolecular transport. Over the last 10 years the details of their activity in both trophic and catabolic processes has been clarified, ...

journal_title：Molecular and cellular endocrinology

authors： Allaerts W,Carmeliet P,Denef C

更新日期：1990-06-18 00:00:00

abstract：:Recent developments in the regulation of testicular Leydig-cell function support the following conclusions. (1) Enzymic activities involved in steroid production in the testis are mainly localized in Leydig cells. The aromatase enzyme complex for oestrogen production appears to be localized in Leydig cells as well as ...

journal_title：Molecular and cellular endocrinology

authors： Rommerts FF,Brinkman AO

更新日期：1981-01-01 00:00:00

abstract：:Recent evidence suggests that the classic gut peptide, Peptide YY (PYY), could play a fundamental role in endocrine pancreatic function. In the present study expression of PYY and its NPY receptors on mouse islets and immortalised rodent and human beta-cells was examined together with the effects of both major circula...

journal_title：Molecular and cellular endocrinology

authors： Khan D,Vasu S,Moffett RC,Irwin N,Flatt PR

更新日期：2016-11-15 00:00:00