Antibacterial and pro-osteogenic effects of β-Defensin-2-loaded mesoporous bioglass.

Abstract:

:The human antimicrobial peptide beta-defensin-2 (hBD2) shows broad antibacterial activity and infrequent bacterial resistance. Here mesoporous bioactive glass (MBG) was loaded with hBD2, forming hBD2-loaded MBG (BD-MBG). The antibacterial and osteogenic effects of BD-MBG were investigated in comparison with MBG and the blank control (BC). The result showed that BD-MBG yielded sustained hBD2 release for more than 7 weeks in vitro, and resulted in significantly lower amounts of viable bacteria and colony forming units, and significantly higher levels of bacterial protein release compared with those in the BC and MBG groups (all p<0.05). Compared with that in the BC group, significantly higher bone marrow stromal cell (BMSC) proliferation rates, alkaline phosphatase (ALP) activity, calcium nodule formation, and expression levels of early and late osteogenic makers were observed after MBG and BD-MBG treatments (p<0.05). Thus, BD-MBG inhibited bacterial growth, damaged their membrane, and promoted early and late osteogenic BMSC differentiation.

journal_name

Dent Mater J

journal_title

Dental materials journal

authors

Ren L,Zhang Z,Deng C,Zhang N,Li D

doi

10.4012/dmj.2020-105

subject

Has Abstract

pub_date

2020-12-24 00:00:00

eissn

0287-4547

issn

1881-1361

pub_type

杂志文章
  • Light activated resin containing organic dyestuff.

    abstract::The physical properties of experimental visible light-activated resins containing dyestuff, which could be quickly discolored by irradiation was, evaluated by analyzing light absorbance characteristics and measuring micro Vickers hardness. The experimental resin, which was prepared by mixing equivalent weights of Bis ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.8.147

    authors: Nakazato J,Itoh K,Wakumoto S

    更新日期:1989-12-01 00:00:00

  • Effect of polymerization cycles on flexural strengths and microhardness of different denture base materials.

    abstract::The purpose of this study was to evaluate the effect of different polymerization cycles on the flexural strengths and microhardness of two denture base materials (Meliodent and Paladent). Heat-polymerized acrylic resin specimens (65.0 mm long×10.0 mm wide×2.5 mm in height) were prepared using different short and long ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2016-023

    authors: Gungor H,Gundogdu M,Alkurt M,Yesil Duymus Z

    更新日期:2017-03-31 00:00:00

  • Effects of light sources and visible light-activated titanium dioxide photocatalyst on bleaching.

    abstract::The objective of this study was to evaluate, using methylene blue (MB), the effects of various light sources on the bleaching action of hydrogen peroxide (H(2)O(2)) with two titanium dioxide (TiO(2)) photocatalysts - an ultraviolet light-activated TiO(2) photocatalyst (UVTiO(2)) versus a visible light-activated TiO(2)...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.28.693

    authors: Suyama Y,Otsuki M,Ogisu S,Kishikawa R,Tagami J,Ikeda M,Kurata H,Cho T

    更新日期:2009-11-01 00:00:00

  • Effect of Cr and Cu addition on corrosion behavior of Ni-Ti alloys.

    abstract::The corrosion behavior of three Ni-Ti alloys with compositions as commercial super-elastic orthodontic wires was investigated using polished plate specimens. Corrosion resistance was estimated by potentiodynamic polarization measurement in 0.9% NaCl and 1% lactic acid solutions and analysis of released metals by atomi...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.17.31

    authors: Iijima M,Endo K,Ohno H,Mizoguchi I

    更新日期:1998-03-01 00:00:00

  • Radio-opacity of core materials for all-ceramic restorations.

    abstract::The aim of this study was to investigate and compare the radio-opacity of core materials for all-ceramic restorations, such as zirconia (NANOZR and Y-TZP) and alumina, against commercially pure titanium (cpTi) and aluminum. X-ray images were taken under general settings using an X-ray film. The X-ray film images were ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2009-54

    authors: Okuda Y,Noda M,Kono H,Miyamoto M,Sato H,Ban S

    更新日期:2010-01-01 00:00:00

  • Development of casting investment preventing blackening of noble metal alloys part 1. Application of developed investment for Ag-Pd-Cu-Au alloy.

    abstract::The objective of this study is to develop a casting investment that prevents the blackening of the cast surface of noble metal alloys. The experimental investments were prepared using a gypsum-bonded investment in which the metallic powders such as boron (B), silicon (Si), aluminum (Al) and titanium (Ti) were added as...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.22.87

    authors: Kakuta K,Nakai A,Goto S,Wakamatsu Y,Yara A,Miyagawa Y,Ogura H

    更新日期:2003-03-01 00:00:00

  • Long-term evaluation of warm-air treatment effect on adaptation of silane-containing universal adhesives to lithium disilicate ceramic.

    abstract::The effect of warm air-blowing on the sealing ability of two one-bottle and one two-bottle silane-containing universal adhesives (UAs) applied to lithium disilicate ceramics (LDS) was evaluated. Tapered cavities prefabricated in LDS blocks (IPS e.max CAD) were treated by the adhesives, air-blown either with normal air...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2020-057

    authors: Kaykhine P,Tichy A,Abdou A,Hosaka K,Foxton RM,Sumi Y,Nakajima M,Tagami J

    更新日期:2020-10-24 00:00:00

  • Influence of silane heat treatment on bond strength of resin cement to a feldspathic ceramic.

    abstract::This study evaluated the influence of heat treatment (HT) of the silane on the microtensile bond strength of resin cement to a feldspathic ceramic. Ceramic (VITA VM7) and composite blocks (N=32) were divided into four groups (n=6 for bond test, n=2 for SEM) at random and subject to following sequence of conditioning: ...

    journal_title:Dental materials journal

    pub_type: 杂志文章,随机对照试验

    doi:

    authors: de Carvalho RF,Martins ME,de Queiroz JR,Leite FP,Ozcan M

    更新日期:2011-01-01 00:00:00

  • Irrigation effectiveness of continuous ultrasonic irrigation system: An ex vivo study.

    abstract::This study evaluated the irrigation extrusion and smear layer (SL) removal of continuous ultrasonic irrigation (CUI). Forty root canals were shaped, embedded in warm sterile saline agar colored with 1% acid red, and distributed into four groups; three groups had the final irrigation sequence of sodium hypochlorite and...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2016-411

    authors: Jamleh A,Suda H,Adorno CG

    更新日期:2018-01-30 00:00:00

  • Effects of experimental pastes containing surface pre-reacted glass ionomer fillers on inhibition of enamel demineralization.

    abstract::This study aimed to evaluate the inhibitory effect of experimental pastes containing surface pre-reacted glass ionomer (S-PRG) fillers on enamel demineralization. Bovine blocks were treated twice a day for 4 days by 7 groups; experimental pastes containing 0-30 wt% S-PRG filler (S00, S01, S05, S10, and S30), deionized...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2016-303

    authors: Nakamura K,Hamba H,Nakashima S,Sadr A,Nikaido T,Oikawa M,Uo M,Tagami J

    更新日期:2017-07-26 00:00:00

  • Titanium removable denture based on a one-metal rehabilitation concept.

    abstract::The use of a single metal for all restorations would be necessary because it protects against metal corrosion caused by the contact of different metals. For this "one-metal rehabilitation" concept, non-alloyed commercially pure (CP) titanium should be used for all restorations. Titanium frameworks have been cast and u...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2017-137

    authors: Ohkubo C,Sato Y,Nishiyama Y,Suzuki Y

    更新日期:2017-09-26 00:00:00

  • Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments.

    abstract::Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and alpha-tricalcium phosphate (alpha-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-alpha-TCP func...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.24.207

    authors: Yuasa T,Miyamoto Y,Kon M,Ishikawa K,Takechi M,Momota Y,Tatehara S,Takano H,Mimamiguchi S,Nagayama M

    更新日期:2005-06-01 00:00:00

  • Temperature dependence of thermal expansion coefficient for palladium-based binary alloy.

    abstract::Temperature dependencies of thermal expansion coefficients for the alloys of Pd-Ag, Pd-Cu and Pd-Co binary systems were measured at temperatures up to 900 degrees C, with heating and cooling rates of 5 degrees C/min. The coefficient of thermal expansion of alloys with no transformation in the temperature range of inte...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.9.47

    authors: Asaoka K,Kuwayama N

    更新日期:1990-06-01 00:00:00

  • Influence of the number of cycles on shear fatigue strength of resin composite bonded to enamel and dentin using dental adhesives in self-etching mode.

    abstract::The influence of the number of cycles on shear fatigue strength to enamel and dentin using dental adhesives in self-etch mode was investigated. A two-step self-etch adhesive and two universal adhesives were used to bond to enamel and dentin in self-etch mode. Initial shear bond strength and shear fatigue strength to e...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2017-012

    authors: Tsujimoto A,Barkmeier WW,Erickson RL,Takamizawa T,Latta MA,Miyazaki M

    更新日期:2018-01-30 00:00:00

  • A study of in vivo degradation of two vital home bleaching gels.

    abstract::This study investigated hydrogen peroxide (H2O2) concentration of two home bleaching gels, in their dispensing syringes and their degradation in vivo in intraoral bleaching trays. Two bleaching gels were studied, 7.5% hydrogen peroxide (HP) and 20% carbamide peroxide (CP). The concentration of H2O2 was determined in d...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2012-317

    authors: Alonso De La Peña V,Rodriguez Carreira A,Corral Aneiros R,López Ratón M,Guitián Rivera F

    更新日期:2013-01-01 00:00:00

  • The effects of glass ionomer and flowable composite liners on the fracture resistance of open-sandwich class II restorations.

    abstract::This in vitro study aimed to investigate the effects of glass-ionomer and flowable composite liners on the fracture resistance of Class II amalgam and composite restorations. Group 1 cavities were restored with amalgam and Group 4 cavities with nanofill composite after the application of a dentin-bonding agent. For th...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2013-053

    authors: Güray Efes B,Yaman BC,Gümüştaş B,Tıryakı M

    更新日期:2013-01-01 00:00:00

  • Activation of matrix-bound endogenous proteases by self-etch adhesives.

    abstract::The study evaluated changes in total enzymatic activity and degradation of demineralized dentin following the application of universal or self-etch adhesives. The universal adhesives -Scotchbond Universal (SU) and All-Bond Universal (ABU) and self-etch adhesives -Adper Easy Bond (EB) and G-aenial Bond (GB) were used f...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2019-304

    authors: Ahmet BSO,Seseogullari-Dirihan R,Tezvergil-Mutluay A

    更新日期:2020-12-03 00:00:00

  • The effect of five kinds of surface treatment agents on the bond strength to various ceramics with thermocycle aging.

    abstract::This study evaluated the effects of ceramic surface treatment agents on shear bond strengths to ceramic materials with and without thermocycling. Ceramic plates were prepared from feldspathic ceramic; AAA, lithium disilicate ceramic material; IPS e.max Press, zirconia ceramic; Lava. Ceramic surfaces were pretreated wi...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2016-383

    authors: Noda Y,Nakajima M,Takahashi M,Mamanee T,Hosaka K,Takagaki T,Ikeda M,Foxton RM,Tagami J

    更新日期:2017-11-29 00:00:00

  • A review of our development of dental adhesives--effects of radical polymerization initiators and adhesive monomers on adhesion.

    abstract::This paper reviews the development of dental adhesives by collating information of related studies from original scientific papers, reviews, and patent literatures. Through our development, novel radical polymerization initiators, adhesive monomers, and microcapsules were synthesized, and their effects on adhesion wer...

    journal_title:Dental materials journal

    pub_type: 杂志文章,评审

    doi:10.4012/dmj.2009-057

    authors: Ikemura K,Endo T

    更新日期:2010-03-01 00:00:00

  • In vitro study of anti-suck-back ability by themselves on new high-speed air turbine handpieces.

    abstract::The anti-suck-back ability of five new high-speed air turbine handpiece models was evaluated in this study. First, suck-back pressure with water displacement within a glass tube was measured. Next, under three different conditions, how many on-off times it takes before fluorescent stains became visible on a piece of g...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2010-008

    authors: Ozawa T,Nakano M,Arai T

    更新日期:2010-11-01 00:00:00

  • Effects of degree of dissociation of acid used on hydrolytic durability of ceramic primer.

    abstract::In this study, effects of the degree of dissociation of acids on the hydrolysis rate of methoxy group in γ-methacryloxypropyltrimethoxysilane (γ-MPS) and the adsorption characteristics of γ-MPS on ceramic surfaces were studied using acetic, phosphoric, and hydrochloric acids. Hydrolytic stability of γ-MPS adsorption l...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2013-319

    authors: Aoki N,Kimoto S,Tanimura H,Nishiyama N,Aida M

    更新日期:2014-01-01 00:00:00

  • Influence of three specimen fixation modes on the micro-tensile bond strength of adhesives to dentin.

    abstract::The aim of this study was to investigate in how far the way the specimen is fixed to the testing device influences the micro-tensile bond strength of adhesives to dentin. Compared to a flat jig, a notched jig enables the specimen to be aligned easier and more accurately perpendicular to the interface, thereby concentr...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.26.694

    authors: Poitevin A,De Munck J,Van Landuyt K,Coutinho E,Peumans M,Lambrechts P,Van Meerbeek B

    更新日期:2007-09-01 00:00:00

  • Clinical evaluation of an electron-ionizing toothbrush with a tooth paste containing stannous fluoride in treatment of dentine hypersensitivity following periodontal surgery.

    abstract::The purpose of this study was to examine the effect of an electro-ionizing toothbrush with stannous fluoride in the treatment of dentin hypersensitivity following periodontal surgery. Thirty-two volunteers with dentin hypersensitivity were divided in two equal groups each using different methods: (Group I) stannous fl...

    journal_title:Dental materials journal

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.4012/dmj.20.164

    authors: Orbak R,Canakçi V,Tezel A

    更新日期:2001-06-01 00:00:00

  • Effect of light intensity on polymerization of light-cured composite resins.

    abstract::The depths of cure and the distributions of degree of conversion (DC), polymerization conversion (PC) and percent pendant double bonds (PDB) of light-cured composite resins cured under various intensities of light were investigated. When the total amount of exposure, represented by the product of the light intensity a...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.13.198

    authors: Nomoto R,Uchida K,Hirasawa T

    更新日期:1994-12-01 00:00:00

  • Multi-purpose bonding performance of newly synthesized phosphonic acid monomers.

    abstract::Multi-purpose bonding performance of three kinds of newly synthesized phosphonic acid monomers was investigated. Methacryloxyalkyl or acryloxyalkyl phosphonoacetates of 6-MHPA, 6-AHPA, 10-MDPA were synthesized in 42.8-51.9% yields with a light yellow viscous liquid, and identified as new compounds by 1H NMR, IR, and e...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.26.105

    authors: Ikemura K,Tay FR,Nishiyama N,Pashley DH,Endo T

    更新日期:2007-01-01 00:00:00

  • Reinforcement mechanism of dentin mechanical properties by intracanal medicaments.

    abstract::The reinforcement mechanism of dentin mechanical properties by intracanal medicaments was investigated. The dumbbell-shaped specimens were prepared from a collagen sheet, demineralized dentin and organic dissolved dentin. After immersing the specimens in intracanal medicaments (eugenol and formocresol), the tensile te...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.18.304

    authors: Nakano F,Takahashi H,Nishimura F

    更新日期:1999-09-01 00:00:00

  • Initial attachment of human oral keratinocytes cultured on zirconia or titanium.

    abstract::Initial attachment of human oral keratinocytes (HOKs) cultured on mirror-surfaced commercially pure titanium (Ti) or yttria-stabilized tetragonal zirconia polycrystals (TZP) was investigated. Numbers of viable attached HOKs, their mRNAs and proteins expression of laminin γ2 and integrin β4 were evaluated using the WST...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2011-189

    authors: Kimura Y,Matsuzaka K,Yoshinari M,Inoue T

    更新日期:2012-01-01 00:00:00

  • Mechanical properties and radiopacity of flowable fiber-reinforced composite.

    abstract::The aim was to evaluate the effect of different zirconia discontinuous fiber fractions on radiopacity and other selected properties of glass discontinuous fiber-reinforced flowable composite (Exp-SFRC). Exp-SFRC was prepared by mixing 30 wt% of resin-matrix and 45 wt% of particulate-fillers to 25 wt% of various weight...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2018-102

    authors: Garoushi S,Vallittu P,Lassila L

    更新日期:2019-03-31 00:00:00

  • Effectiveness of sodium hypochlorite and sulfinic acid sodium salt treatment on dentin-resin bonding: Long-term durability of one-step self-etching adhesive.

    abstract::This study aimed to evaluate the effect of sulfinic acid sodium salt interposition after acid and sodium hypochlorite treatment (NC treatment) on dentin bonding durability using a mild type one-step self-etching adhesive. Fifteen human third molars were randomly assigned into three experimental groups according to den...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2017-105

    authors: Nakatani H,Mine A,Matsumoto M,Kabetani T,Kawaguchi-Uemura A,Higashi M,Tajiri Y,Imai D,Hagino R,Minamino T,Miura J,Yatani H

    更新日期:2017-11-29 00:00:00

  • A study of self-adhesive resin cements for bonding to silver-palladium-copper-gold alloy -- effect of including primer components in cement base.

    abstract::This study investigated the efficacies of adhesive resin cements (Clearfil SA Luting, Maxcem, G-CEM, RelyX Unicem Clicker, Vitremer Paste) for bonding to Ag-Pd-Cu-Au alloy not surface-pretreated with metal primer. For control, Panavia F 2.0 -developed for use with a proprietary metal primer, Alloy Primer- was tested w...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2010-093

    authors: Muraguchi K,Minami H,Minesaki Y,Suzuki S,Tanaka T

    更新日期:2011-01-01 00:00:00