Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.

abstract：:Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade ...

journal_title：AMB Express

authors： Liu Z,Dang K,Li C,Gao J,Wang H,Gao Y,Zhao B,Fan P,Qian A

更新日期：2020-10-31 00:00:00

abstract：:A novel strain of Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize medium chain-length polyhydroxyalkanoates (mcl-PHAs). P.putida LS46 was differentiated from other P.putida strains on the basis of cpn60 (UT). The complete genome of P.putida LS46 was sequenced and annotate...

journal_title：AMB Express

authors： Sharma PK,Fu J,Zhang X,Fristensky B,Sparling R,Levin DB

更新日期：2014-05-22 00:00:00

abstract：:Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a swine respiratory disease with an important impact around the world either as a single infection or part of the porcine respiratory disease complex. The data of interaction between hosts and pathogens has becoming more crucial for exploration...

journal_title：AMB Express

authors： Zhu R,Bao C,Liu B,Xiao J,Sun C,Feng X,Langford PR,Li Y,Lei L

更新日期：2020-07-06 00:00:00

abstract：:Gene therapy and viral vaccine are becoming attractive therapeutic options for the treatment of different malignant diseases. Viral vector productions are often using static culture vessels and small volume stainless steel bioreactors (SSB). However, the yield of each vessel can be relatively low and multiple vessels ...

journal_title：AMB Express

authors： Yang J,Guertin P,Jia G,Lv Z,Yang H,Ju D

更新日期：2019-05-24 00:00:00

abstract：:Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify a...

journal_title：AMB Express

authors： Wang Y,Zhao B,Liu Y,Mao L,Zhang X,Meng W,Liu K,Chu J

更新日期：2020-01-14 00:00:00

abstract：:Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In ...

journal_title：AMB Express

authors： Kitahara N,Morisaka H,Aoki W,Takeda Y,Shibasaki S,Kuroda K,Ueda M

更新日期：2015-12-01 00:00:00

abstract：:Clean coal technologies (e.g. coal biosolubilization) are of essential value, especially in Europe, where coal is the national wealth and other energy sources like crude oil are not available. Fusarium oxysporum LOCK 1134, the strain isolated from brown coal, efficiently biosolubilizes lignite. The obtained liquefied ...

journal_title：AMB Express

authors： Kwiatos N,Jędrzejczak-Krzepkowska M,Strzelecki B,Bielecki S

更新日期：2018-08-22 00:00:00

abstract：:Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. How...

journal_title：AMB Express

authors： Hu X,Xu N,Yang X,Hu X,Zheng Y,Zhang Q

更新日期：2020-10-04 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：:Aglycon protopanaxatriol (APPT) has valuable pharmacological effects such as anti-inflammatory and anti-stress activities. However, the complete conversion of all typical glycosylated protopanaxatriol ginsenosides to APPT has not been achieved to date. β-Glycosidase from the hyperthermophilic bacterium Dictyoglomus tu...

journal_title：AMB Express

authors： Yang EJ,Kim TH,Shin KC,Oh DK

更新日期：2018-01-24 00:00:00

abstract：:Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus...

journal_title：AMB Express

authors： Lu F,Huang Y,Zhang X,Wang Z

更新日期：2017-12-01 00:00:00

abstract：:Cellulose degrading enzymes usually have a two-domain structure consisting of a catalytic domain and a non-catalytic carbohydrate-binding module. Although it is well known the importance of those modules in cell wall degrading process, their function is not yet fully understood. Here, we analyze the cellulose-hydrolys...

journal_title：AMB Express

authors： Mello BL,Polikarpov I

更新日期：2014-04-25 00:00:00

abstract：:Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

journal_title：AMB Express

authors： Fikry S,Khalil N,Salama O

更新日期：2019-03-26 00:00:00

abstract：:The discovery of aerobic and anammox bacteria capable of generating methane in bio-filters in freshwater aquaculture systems is generating interest in studies to understand the activity, diversity, distribution and roles of these environmental bacteria. In this study, we used microbial enrichment of bio-filters to ass...

journal_title：AMB Express

authors： Kamira B,Shi LL,Fan LM,Zhang C,Zheng Y,Song C,Meng SL,Hu GD,Bing XW,Chen ZJ,Xu P

更新日期：2018-08-28 00:00:00

abstract：:The utilization of inoculants containing Azospirillum is becoming more popular due to increasing reports of expressive gains in grain yields. However, incompatibility with pesticides used in seed treatments represents a main limitation for a successful inoculation. Therefore, in this study we searched for alternatives...

journal_title：AMB Express

authors： Fukami J,Nogueira MA,Araujo RS,Hungria M

更新日期：2016-03-01 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：:Phasins are amphiphilic proteins involved in the regulation of the number and size of polyhydroxybutyrate (PHB) granules. The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and energy source. By analyzing the genome, we identified six genes that cod...

journal_title：AMB Express

authors： Martínez-Martínez MLA,González-Pedrajo B,Dreyfus G,Soto-Urzúa L,Martínez-Morales LJ

更新日期：2019-09-25 00:00:00

abstract：:Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel ...

journal_title：AMB Express

authors： Sakai K,Yamaguchi A,Tsutsumi S,Kawai Y,Tsuzuki S,Suzuki H,Jindou S,Suzuki Y,Kajimura H,Kato M,Shimizu M

更新日期：2020-05-24 00:00:00

abstract：: Hydroquinone-1,2-dioxygenase, an enzyme involved in the degradation of alkylphenols in Sphingomonas sp. strain TTNP3 was purified to apparent homogeneity. The extradiol dioxygenase catalyzed the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydr...

journal_title：AMB Express

authors： Kolvenbach BA,Lenz M,Benndorf D,Rapp E,Fousek J,Vlcek C,Schäffer A,Gabriel FL,Kohler HP,Corvini PF

更新日期：2011-05-27 00:00:00

abstract：:The effects of inlet VOCs (Volatile Organic Compounds) shifts on microbial community structure in a biofiltration system were investigated. A lab-scale biofilter was set up to treat eight VOCs sequentially. Short declines in removal efficiency appeared after VOCs shifts and then later recovered. The number of OTUs in ...

journal_title：AMB Express

authors： Lu L,Wang G,Yeung M,Xi J,Hu HY

更新日期：2018-10-03 00:00:00

abstract：:Disentangling the interactions between cyanobacteria and associated bacterial community is important for understanding the mechanisms that mediate the formation of cyanobacterial blooms in freshwater ecosystems. Despite the fact that a metagenomic approach enables researchers to profile the structure of microbial comm...

journal_title：AMB Express

authors： Ren M,Zhang G,Ye Z,Qiao Z,Xie M,Lin Y,Li T,Zhao J

更新日期：2017-11-02 00:00:00

abstract：:Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments. To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes ...

journal_title：AMB Express

authors： Lyu T,Liu G,Zhang H,Wang L,Zhou S,Dou H,Pang B,Sha W,Zhang H

更新日期：2018-08-02 00:00:00

abstract：:Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS m...

journal_title：AMB Express

authors： Liang J,Cheng T,Huang Y,Liu J

更新日期：2018-05-28 00:00:00

abstract：:Calebin-A is a curcuminoid compound reported to be present in Curcuma longa rhizome. The current study was aimed to isolate and characterize calebin-A from Curcuma caesia rhizome and its production through biotransformation approach using endophytic fungus. C. caesia rhizomes of different ages were subjected to analys...

journal_title：AMB Express

authors： Majeed A,Majeed M,Thajuddin N,Arumugam S,Ali F,Beede K,Adams SJ,Gnanamani M

更新日期：2019-05-29 00:00:00

abstract：:The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes...

journal_title：AMB Express

authors： Bartolo-Aguilar Y,Dendooven L,Chávez-Cabrera C,Flores-Cotera LB,Hidalgo-Lara ME,Villa-Tanaca L,Marsch R

更新日期：2017-12-01 00:00:00

abstract：:In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over a...

journal_title：AMB Express

authors： Ji JH,Zhou L,Mbadinga SM,Irfan M,Liu YF,Pan P,Qi ZZ,Chen J,Liu JF,Yang SZ,Gu JD,Mu BZ

更新日期：2020-02-01 00:00:00

abstract：:Aspergillus aculeatus β-glucosidase 1 (AaBGL1), which promotes cellulose hydrolysis by Trichoderma cellulase system, was characterized and compared some properties to a commercially supplied orthologue in A. niger (AnBGL) to elucidate advantages of recombinant AaBGL1 (rAaBGL1) for synergistic effect on Trichoderma enz...

journal_title：AMB Express

authors： Baba Y,Sumitani JI,Tani S,Kawaguchi T

更新日期：2015-01-24 00:00:00

abstract：:Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase a...

journal_title：AMB Express

authors： Bayitse R,Hou X,Bjerre AB,Saalia FK

更新日期：2015-12-01 00:00:00

abstract：:The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was in three distinct growth stages: cell growth, lipid accumulation and a final lipid turnover stage. Most of ...

journal_title：AMB Express

authors： Safdar W,Shamoon M,Zan X,Haider J,Sharif HR,Shoaib M,Song Y

更新日期：2017-12-01 00:00:00

abstract：:A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl2 treatment of the protoplasts and plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by a homologous promoter. During re...

journal_title：AMB Express

authors： Honda Y,Tanigawa E,Tsukihara T,Nguyen DX,Kawabe H,Sakatoku N,Watari J,Sato H,Yano S,Tachiki T,Irie T,Watanabe T,Watanabe T

更新日期：2019-06-24 00:00:00