Response of microbial community structure and metabolic profile to shifts of inlet VOCs in a gas-phase biofilter.

abstract：:Recombinant antibodies are used with great success in many different diagnostic and therapeutic applications. A variety of protein expression systems are available, but nowadays almost all therapeutic antibodies are produced in mammalian cell lines due to their complex structure and glycosylation requirements. However...

journal_title：AMB Express

authors： Blanco-Toribio A,Álvarez-Cienfuegos A,Sainz-Pastor N,Merino N,Compte M,Sanz L,Blanco FJ,Álvarez-Vallina L

更新日期：2015-12-01 00:00:00

abstract：:Increasing evidences suggest that intestinal microbiota balance closely correlated with host's health status could affected by external environment. Integrated crayfish-rice cultivation model is a highly efficient artificial ecosystem widely practiced in subtropical China. Less information is available to estimate the...

journal_title：AMB Express

authors： Shui Y,Guan ZB,Liu GF,Fan LM

更新日期：2020-01-14 00:00:00

abstract：:The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes...

journal_title：AMB Express

authors： Bartolo-Aguilar Y,Dendooven L,Chávez-Cabrera C,Flores-Cotera LB,Hidalgo-Lara ME,Villa-Tanaca L,Marsch R

更新日期：2017-12-01 00:00:00

abstract：:Ginsenoside compound K has been used as a key nutritional and cosmetic component because of its anti-fatigue and skin anti-aging effects. β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is known as the most efficient enzyme for compound K production. The hydrolytic pathway from ginsenoside Rb1 to compound K via Rd...

journal_title：AMB Express

authors： Shin KC,Choi HY,Seo MJ,Oh DK

更新日期：2017-10-04 00:00:00

abstract：:Biological technologies for recycling rare metals, which are essential for high-tech products, have attracted much attention because they could prove to be more environmentally friendly and energy-saving than other methods. We have developed biological recycling technologies by cell surface engineering for the selecti...

journal_title：AMB Express

authors： Ito R,Kuroda K,Hashimoto H,Ueda M

更新日期：2016-12-01 00:00:00

abstract：:The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial ...

journal_title：AMB Express

authors： Kaur N,Arora DS

更新日期：2020-11-11 00:00:00

abstract：:The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA...

journal_title：AMB Express

authors： Hu Z,Ma D,Niu H,Chang J,Yu J,Tong Q,Li S

更新日期：2021-01-06 00:00:00

abstract：:The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...

journal_title：AMB Express

authors： Ibrahim MHA,Lebbe L,Willems A,Steinbüchel A

更新日期：2016-12-01 00:00:00

abstract：:An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experiment...

journal_title：AMB Express

authors： Abdelrazek NA,Elkhatib WF,Raafat MM,Aboulwafa MM

更新日期：2020-04-15 00:00:00

abstract：:Although high levels of staphylococcal phenol-soluble modulins (PSMs) in clinical methicillin-resistant Staphylococcus aureus (MRSA) has been shown to correlate with bacterial virulence, the PSMs expression in foodborne Staphylococcus aureus (S. aureus), as well as its association with staphylococcal food poisoning (S...

journal_title：AMB Express

authors： Wu X,Yang M,Fang X,Zhen S,Zhang J,Yang X,Qiao L,Yang Y,Zhang C

更新日期：2018-11-22 00:00:00

abstract：:Herbicides are important tools for weed control in modern agriculture. In the search for potential herbicidal natural products from fungal species, harzianum A and B were identified from the biofertilizer fungus, Trichoderma brevicompactum. In the phytotoxicity assays on the dicot species Brassica chinensis, harzianum...

journal_title：AMB Express

authors： Yin M,Fasoyin OE,Wang C,Yue Q,Zhang Y,Dun B,Xu Y,Zhang L

更新日期：2020-07-01 00:00:00

abstract：:This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine. In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccha...

journal_title：AMB Express

authors： Benito Á,Calderón F,Benito S

更新日期：2019-02-02 00:00:00

abstract：:Thermomonospora curvata is a thermophilic actinomycete phylogenetically related to Thermobifida fusca that produces extracellular hydrolases capable of degrading synthetic polyesters. Analysis of the genome of T. curvata DSM43183 revealed two genes coding for putative polyester hydrolases Tcur1278 and Tcur0390 sharing...

journal_title：AMB Express

authors： Wei R,Oeser T,Then J,Kühn N,Barth M,Schmidt J,Zimmermann W

更新日期：2014-06-03 00:00:00

abstract：:Protein-ligand docking plays an important role in computer-aided pharmaceutical development. Protein-ligand docking can be defined as a search algorithm with a scoring function, whose aim is to determine the conformation of the ligand and the receptor with the lowest energy. Hence, to improve an efficient algorithm ha...

journal_title：AMB Express

authors： Guan B,Zhang C,Ning J

更新日期：2017-09-13 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

journal_title：AMB Express

authors： Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

更新日期：2012-04-25 00:00:00

abstract：:The utilization of inoculants containing Azospirillum is becoming more popular due to increasing reports of expressive gains in grain yields. However, incompatibility with pesticides used in seed treatments represents a main limitation for a successful inoculation. Therefore, in this study we searched for alternatives...

journal_title：AMB Express

authors： Fukami J,Nogueira MA,Araujo RS,Hungria M

更新日期：2016-03-01 00:00:00

abstract：:This study was demonstrated with a coculture fermentation system using sugar beet pulp (SBP) as a carbon source combining the cellulose-degrading bacterium Clostridium cellulovorans with microbial flora of methane production (MFMP) for the direct conversion of cellulosic biomass to methane (CH4). The MFMP was taken fr...

journal_title：AMB Express

authors： Tomita H,Okazaki F,Tamaru Y

更新日期：2019-02-18 00:00:00

abstract：:The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell b...

journal_title：AMB Express

authors： Werner A,Otte KL,Stahlhut G,Pöggeler S

更新日期：2020-12-21 00:00:00

abstract：:With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae...

journal_title：AMB Express

authors： Eibl JK,Corcoran JD,Senhorinho GN,Zhang K,Hosseini NS,Marsden J,Laamanen CA,Scott JA,Ross GM

更新日期：2014-03-26 00:00:00

abstract：: Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP). The goal was to produce and test proteins that inhibit both the coffee berry borer insect Hypothenemus ha...

journal_title：AMB Express

authors： Martínez CP,Echeverri C,Florez JC,Gaitan AL,Góngora CE

更新日期：2012-03-30 00:00:00

abstract：:Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli ar...

journal_title：AMB Express

authors： Mozejko-Ciesielska J,Dabrowska D,Szalewska-Palasz A,Ciesielski S

更新日期：2017-12-01 00:00:00

abstract：:Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5...

journal_title：AMB Express

authors： Duarte JC,Valença GP,Moran PJ,Rodrigues JA

更新日期：2015-02-20 00:00:00

abstract：:High-performance liquid chromatography-mass spectrometry (LC-MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis ...

journal_title：AMB Express

authors： Gu F,Chen Y,Hong Y,Fang Y,Tan L

更新日期：2017-12-01 00:00:00

abstract：:With the aim to develop biocatalysts for enhanced hydrolysis of (hemi)cellulose into monosaccharides, random diversity by directed evolution was introduced in the gene coding for the endo-β-1,4-glucanase from Streptomyces sp. G12 which had been recombinantly expressed in Escherichia coli and named rCelStrep. The main ...

journal_title：AMB Express

authors： Cecchini DA,Pepe O,Pennacchio A,Fagnano M,Faraco V

更新日期：2018-05-05 00:00:00

abstract：:A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl2 treatment of the protoplasts and plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by a homologous promoter. During re...

journal_title：AMB Express

authors： Honda Y,Tanigawa E,Tsukihara T,Nguyen DX,Kawabe H,Sakatoku N,Watari J,Sato H,Yano S,Tachiki T,Irie T,Watanabe T,Watanabe T

更新日期：2019-06-24 00:00:00

abstract：:The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs functi...

journal_title：AMB Express

authors： Aghajanyan A,Gabrielyan L,Schubert R,Trchounian A

更新日期：2020-04-07 00:00:00

abstract：:Green alga Chlamydomonas reinhardtii has gained interest as a sustainable resource because it can be easily grown using CO2 as a carbon source owing to its high CO2 assimilating activity. Although the robustness of the cell wall of C. reinhardtii makes it difficult to extract its intracellular products, such property ...

journal_title：AMB Express

authors： Nakanishi A,Iritani K,Sakihama Y,Ozawa N,Mochizuki A,Watanabe M

更新日期：2020-06-10 00:00:00

abstract：: Biocathode MFCs using microorganisms as catalysts have important advantages in lowering cost and improving sustainability. Electrode materials and microbial synergy determines biocathode MFCs performance. In this study, four materials, granular activated carbon (GAC), granular semicoke (GS), granular graphite (GG) an...

journal_title：AMB Express

authors： Sun Y,Wei J,Liang P,Huang X

更新日期：2012-03-29 00:00:00

abstract：:Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS m...

journal_title：AMB Express

authors： Liang J,Cheng T,Huang Y,Liu J

更新日期：2018-05-28 00:00:00