Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.

abstract：:This study investigated the effects of the rumen fungus Piromyces sp. CN6 CGMCC 14449 as a silage additive on the fermentation quality, nutrient composition and in vitro digestibility of whole crop maize silage. Whole crop maize served as the silage material and was vacuum packed in polyethylene bags. Three ensiling t...

journal_title：AMB Express

authors： Wang D,Zhao C,Liu S,Zhang T,Yao J,Cao Y

更新日期：2019-07-29 00:00:00

abstract：:The basidiospores of most Agaricomycetes are ballistospores. They are propelled off from their basidia at maturity when Buller's drop develops at high humidity at the hilar spore appendix and fuses with a liquid film formed on the adaxial side of the spore. Spores are catapulted into the free air space between hymenia...

journal_title：AMB Express

authors： Lakkireddy K,Kües U

更新日期：2017-12-01 00:00:00

abstract：: Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay for measuring glucose uptake in skeletal muscle cells was established. During the screening studies to discov...

journal_title：AMB Express

authors： Kulkarni-Almeida AA,Brahma MK,Padmanabhan P,Mishra PD,Parab RR,Gaikwad NV,Thakkar CS,Tokdar P,Ranadive PV,Nair AS,Damre AA,Bahirat UA,Deshmukh NJ,Doshi LS,Dixit AV,George SD,Vishwakarma RA,Nemmani KV,Mahajan GB

更新日期：2011-11-21 00:00:00

abstract：: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

journal_title：AMB Express

authors： Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

更新日期：2012-04-25 00:00:00

abstract：:Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attemp...

journal_title：AMB Express

authors： Watanabe Y,Kuroda K,Tatemichi Y,Nakahara T,Aoki W,Ueda M

更新日期：2020-04-15 00:00:00

abstract：:Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natur...

journal_title：AMB Express

authors： Roth T,Beer B,Pick A,Sieber V

更新日期：2017-12-01 00:00:00

abstract：:Fusicocca-2,10(14)-diene (FCdiene) is a diterpene which is interesting as a precursor of the anti-cancer drug fusicoccin A and therefore for pharmaceutical applications. Production of FCdiene using a genetically modified Saccharomyces cerevisiae has been previously demonstrated with batch cultivations with a product c...

journal_title：AMB Express

authors： Halka LM,Nowacki C,Kleinschmidt A,Koenen K,Wichmann R

更新日期：2018-08-22 00:00:00

abstract：:Recombinant antibodies are used with great success in many different diagnostic and therapeutic applications. A variety of protein expression systems are available, but nowadays almost all therapeutic antibodies are produced in mammalian cell lines due to their complex structure and glycosylation requirements. However...

journal_title：AMB Express

authors： Blanco-Toribio A,Álvarez-Cienfuegos A,Sainz-Pastor N,Merino N,Compte M,Sanz L,Blanco FJ,Álvarez-Vallina L

更新日期：2015-12-01 00:00:00

abstract：:Spinal cord injury (SCI) is involved with abnormal expression of miRNAs (miRs) which are responsible for some IIry injury responses which include apoptosis, inflammation and oxidative stress. Mechanisms involving miRs induced apoptosis still needs to be investigated. In the present work we developed a rat model of SCI...

journal_title：AMB Express

authors： An Y,Li J,Yuan Q,Fan M

更新日期：2020-06-15 00:00:00

abstract：:Phasins are amphiphilic proteins involved in the regulation of the number and size of polyhydroxybutyrate (PHB) granules. The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and energy source. By analyzing the genome, we identified six genes that cod...

journal_title：AMB Express

authors： Martínez-Martínez MLA,González-Pedrajo B,Dreyfus G,Soto-Urzúa L,Martínez-Morales LJ

更新日期：2019-09-25 00:00:00

abstract：:The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell b...

journal_title：AMB Express

authors： Werner A,Otte KL,Stahlhut G,Pöggeler S

更新日期：2020-12-21 00:00:00

abstract：:Poly-gamma-glutamic acid (PGA) is a promising bio-based polymer that shares many functions with poly (acrylic acid) and its derivatives. Thus, technologies for efficient production and molecular size control of PGA are required to expand the application of this useful biopolymer. In Bacillus strains, PGA is synthesize...

journal_title：AMB Express

authors： Sawada K,Araki H,Takimura Y,Masuda K,Kageyama Y,Ozaki K,Hagihara H

更新日期：2018-07-03 00:00:00

abstract：:The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs functi...

journal_title：AMB Express

authors： Aghajanyan A,Gabrielyan L,Schubert R,Trchounian A

更新日期：2020-04-07 00:00:00

abstract：:The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

journal_title：AMB Express

authors： Shonyela SM,Feng B,Yang W,Yang G,Wang C

更新日期：2020-04-17 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：:High temperature causes ubiquitous environmental stress to microorganisms, but studies have not fully explained whether and to what extent heat shock would affect genome stability. Hence, this study explored heat-shock-induced genomic alterations in the yeast Saccharomyces cerevisiae. Using genetic screening systems a...

journal_title：AMB Express

authors： Shen L,Wang YT,Tang XX,Zhang K,Wang PM,Sui Y,Zheng DQ

更新日期：2020-08-17 00:00:00

abstract：:Ginsenoside compound K has been used as a key nutritional and cosmetic component because of its anti-fatigue and skin anti-aging effects. β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is known as the most efficient enzyme for compound K production. The hydrolytic pathway from ginsenoside Rb1 to compound K via Rd...

journal_title：AMB Express

authors： Shin KC,Choi HY,Seo MJ,Oh DK

更新日期：2017-10-04 00:00:00

abstract：:Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of ...

journal_title：AMB Express

authors： Gao D,Chang K,Ding G,Wu H,Chen Y,Jia M,Liu X,Wang S,Jin Y,Pan H,Li H

更新日期：2019-05-24 00:00:00

abstract：:Bacillus thuringiensis is a Gram-positive soil bacterium that is known to be a bacterial biopesticide that produces insecticidal proteins called crystal proteins (Cry). In the insecticidal process, chitinases are suggested to perforate the peritrophic membrane barrier to facilitate the invasion of the Cry proteins int...

journal_title：AMB Express

authors： Honda S,Kunii T,Nohara K,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

更新日期：2017-12-01 00:00:00

abstract：:The discovery of aerobic and anammox bacteria capable of generating methane in bio-filters in freshwater aquaculture systems is generating interest in studies to understand the activity, diversity, distribution and roles of these environmental bacteria. In this study, we used microbial enrichment of bio-filters to ass...

journal_title：AMB Express

authors： Kamira B,Shi LL,Fan LM,Zhang C,Zheng Y,Song C,Meng SL,Hu GD,Bing XW,Chen ZJ,Xu P

更新日期：2018-08-28 00:00:00

abstract：:In the present work, a remediation bioprocess based on the use of a native isolate of Chlorella vulgaris immobilized in an alginate matrix inside a polylactic acid (PLA) device is proposed. This microalga immobilized in alginate beads was previously shown to be useful for the reduction of several chemical and microbia...

journal_title：AMB Express

authors： Marconi PL,Trentini A,Zawoznik M,Nadra C,Mercadé JM,Sánchez Novoa JG,Orozco D,Groppa MD

更新日期：2020-08-15 00:00:00

abstract：:Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L...

journal_title：AMB Express

authors： Ece S,Lambertz C,Fischer R,Commandeur U

更新日期：2017-12-01 00:00:00

abstract：:Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. Lipophilic dyes such as Nile red and BODIPY have been used to quantify intracellular PHB, but their uses have often been limited in...

journal_title：AMB Express

authors： Choi JE,Na HY,Yang TH,Rhee SK,Song JK

更新日期：2015-12-01 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：: In this study manganese peroxidase (MnP) enzymes from selected white-rot fungi were isolated and compared for potential future recombinant production. White-rot fungi were cultivated in small-scale in liquid media and a simplified process was established for the purification of extracellular enzymes.Five lignin degra...

journal_title：AMB Express

authors： Järvinen J,Taskila S,Isomäki R,Ojamo H

更新日期：2012-11-29 00:00:00

abstract：:Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have...

journal_title：AMB Express

authors： Almaraz-Delgado AL,Flores-Uribe J,Pérez-España VH,Salgado-Manjarrez E,Badillo-Corona JA

更新日期：2014-08-13 00:00:00

abstract：:Plant extracts serve as reducing and coating agents and are, therefore, commonly employed for the generation of silver (Ag) nanoparticles (NPs). Plant extract mediated synthesis of Ag NPs is a green, environmentally friendly and cost-effective technique which offers a new and potential alternative to chemically synthe...

journal_title：AMB Express

authors： Hambardzumyan S,Sahakyan N,Petrosyan M,Nasim MJ,Jacob C,Trchounian A

更新日期：2020-09-05 00:00:00

abstract：:The zygomycete fungus Lichtheimia ramosa H71D, isolated from sugarcane bagasse compost, was identified by applying phylogenetic analysis based on the DNA sequence of the Internal Transcribed Spacer (ITS), and subsequent secondary structure analysis of ITS2. L. ramosa H71D was able to grow over a wide range of temperat...

journal_title：AMB Express

authors： Alvarez-Zúñiga MT,Santiago-Hernández A,Rodríguez-Mendoza J,Campos JE,Pavón-Orozco P,Trejo-Estrada S,Hidalgo-Lara ME

更新日期：2017-11-02 00:00:00

abstract：:Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli ar...

journal_title：AMB Express

authors： Mozejko-Ciesielska J,Dabrowska D,Szalewska-Palasz A,Ciesielski S

更新日期：2017-12-01 00:00:00

abstract：:Cardiac arrhythmia is an irregular heart rhythm that can lead to serious heart conditions and various organ disorders, and may cause sudden cardiac death. Catalpol belongs to the iridoid glycoside family and is highly abundant in Rehmannia glutinosa Libosch. The study included five groups such as group I (normal contr...

journal_title：AMB Express

authors： Yang L,Feng X,Li Y,Zhang S,Ying Y

更新日期：2020-03-20 00:00:00