Abstract:
:Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L-1 following purification by one-step affinity chromatography. Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparation was active over a broad pH range and at high temperatures, suggesting it would be suitable for biomass degradation. The reusability of ScLac was also demonstrated by immobilizing the enzyme on agarose beads with a binding yield of 33%, and by the synthesis of cross-linked enzyme aggregates with an initial activity recovery of 72%.
journal_name
AMB Expressjournal_title
AMB Expressauthors
Ece S,Lambertz C,Fischer R,Commandeur Udoi
10.1186/s13568-017-0387-0subject
Has Abstractpub_date
2017-12-01 00:00:00pages
86issue
1issn
2191-0855pii
10.1186/s13568-017-0387-0journal_volume
7pub_type
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