Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara).

abstract：:The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broa...

journal_title：AMB Express

authors： Ao XL,Yu X,Wu DT,Li C,Zhang T,Liu SL,Chen SJ,He L,Zhou K,Zou LK

更新日期：2018-06-12 00:00:00

abstract：:Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli ar...

journal_title：AMB Express

authors： Mozejko-Ciesielska J,Dabrowska D,Szalewska-Palasz A,Ciesielski S

更新日期：2017-12-01 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：:Young barley leaf extract (YBL) contains beneficial substances such as fructans, minerals, and vitamins. The effects of YBL administration on the human colonic microbiota and its production of metabolites were evaluated using an in vitro model culture system. Fermentations were started by inoculating fecal samples fro...

journal_title：AMB Express

authors： Sasaki D,Sasaki K,Kadowaki Y,Aotsuka Y,Kondo A

更新日期：2019-11-13 00:00:00

abstract：: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

journal_title：AMB Express

authors： Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

更新日期：2012-04-25 00:00:00

abstract：:Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

journal_title：AMB Express

authors： Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

更新日期：2020-02-25 00:00:00

abstract：:Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressu...

journal_title：AMB Express

authors： Sakudo A,Yagyu Y

更新日期：2021-01-09 00:00:00

abstract：:A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase...

journal_title：AMB Express

authors： Knudsen JD,Carlquist M,Gorwa-Grauslund M

更新日期：2014-10-30 00:00:00

abstract：:Lactobacilli, as the largest group of lactic acid bacteria, produce large amounts of antimicrobial metabolites such as organic acids, fatty acids, ammonia, hydrogen peroxide, diacetyl and bacteriocin, which inhibit the growth of pathogenic bacteria and increase shelf life of food. The aim of this study was to identify...

journal_title：AMB Express

authors： Azizi F,Habibi Najafi MB,Edalatian Dovom MR

更新日期：2017-09-18 00:00:00

abstract：:The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternativ...

journal_title：AMB Express

authors： da Cruz Ramos GF,Ramos PL,Passarini MRZ,Vieira Silveira MA,Okamoto DN,de Oliveira LCG,Zezzo LV,Marem A,Santos Rocha RC,da Cruz JB,Juliano L,de Vasconcellos SP

更新日期：2016-03-01 00:00:00

abstract：:This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine. In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccha...

journal_title：AMB Express

authors： Benito Á,Calderón F,Benito S

更新日期：2019-02-02 00:00:00

abstract：:The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA...

journal_title：AMB Express

authors： Hu Z,Ma D,Niu H,Chang J,Yu J,Tong Q,Li S

更新日期：2021-01-06 00:00:00

abstract：: Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay for measuring glucose uptake in skeletal muscle cells was established. During the screening studies to discov...

journal_title：AMB Express

authors： Kulkarni-Almeida AA,Brahma MK,Padmanabhan P,Mishra PD,Parab RR,Gaikwad NV,Thakkar CS,Tokdar P,Ranadive PV,Nair AS,Damre AA,Bahirat UA,Deshmukh NJ,Doshi LS,Dixit AV,George SD,Vishwakarma RA,Nemmani KV,Mahajan GB

更新日期：2011-11-21 00:00:00

abstract：:The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is comparatively simple, well characterized and can be manipulated easily...

journal_title：AMB Express

authors： Kangwa M,Yelemane V,Polat AN,Gorrepati KD,Grasselli M,Fernández-Lahore M

更新日期：2015-12-01 00:00:00

abstract：:Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liqui...

journal_title：AMB Express

authors： Huang L,Sun N,Ban L,Wang Y,Yang H

更新日期：2019-01-07 00:00:00

abstract：:Green alga Chlamydomonas reinhardtii has gained interest as a sustainable resource because it can be easily grown using CO2 as a carbon source owing to its high CO2 assimilating activity. Although the robustness of the cell wall of C. reinhardtii makes it difficult to extract its intracellular products, such property ...

journal_title：AMB Express

authors： Nakanishi A,Iritani K,Sakihama Y,Ozawa N,Mochizuki A,Watanabe M

更新日期：2020-06-10 00:00:00

abstract：:Crude enzyme from Geotrichum candidum S12 exhibited high activity towards hexanol at pH 4.0, distinguishing it from currently known enzymes. To identify the dominant enzyme contributing to this activity, the crude enzyme extract was separated into different fractions by ammonium sulfate precipitation, MonoQ anion-exch...

journal_title：AMB Express

authors： Zhu J,Lu K,Xu X,Wang X,Shi J

更新日期：2017-12-01 00:00:00

abstract：:This study investigated the effects of the rumen fungus Piromyces sp. CN6 CGMCC 14449 as a silage additive on the fermentation quality, nutrient composition and in vitro digestibility of whole crop maize silage. Whole crop maize served as the silage material and was vacuum packed in polyethylene bags. Three ensiling t...

journal_title：AMB Express

authors： Wang D,Zhao C,Liu S,Zhang T,Yao J,Cao Y

更新日期：2019-07-29 00:00:00

abstract：:Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L...

journal_title：AMB Express

authors： Kang TS,Korber DR,Tanaka T

更新日期：2013-01-31 00:00:00

abstract：:The tissues of marine invertebrates are colonized by species-rich microbial communities. The dysbiosis of host's microbiota is tightly associated with the invertebrate diseases. Yesso scallop (Patinopecten yessoensis), one of the most important maricultured scallops in northern China, has recently suffered massive sum...

journal_title：AMB Express

authors： Yu Z,Liu C,Fu Q,Lu G,Han S,Wang L,Song L

更新日期：2019-09-14 00:00:00

abstract：:Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this ext...

journal_title：AMB Express

authors： Roberts DP,Selmer K,Lupitskyy R,Rice C,Buyer JS,Maul JE,Lakshman DK,DeSouza J

更新日期：2021-01-06 00:00:00

abstract：:Disentangling the interactions between cyanobacteria and associated bacterial community is important for understanding the mechanisms that mediate the formation of cyanobacterial blooms in freshwater ecosystems. Despite the fact that a metagenomic approach enables researchers to profile the structure of microbial comm...

journal_title：AMB Express

authors： Ren M,Zhang G,Ye Z,Qiao Z,Xie M,Lin Y,Li T,Zhao J

更新日期：2017-11-02 00:00:00

abstract：:The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs functi...

journal_title：AMB Express

authors： Aghajanyan A,Gabrielyan L,Schubert R,Trchounian A

更新日期：2020-04-07 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the prese...

journal_title：AMB Express

authors： Lee JH,Kim YG,Gwon G,Wood TK,Lee J

更新日期：2016-12-01 00:00:00

abstract：:Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus...

journal_title：AMB Express

authors： Lu F,Huang Y,Zhang X,Wang Z

更新日期：2017-12-01 00:00:00

abstract：:Here, we report a novel protein elicitor from Bacillus subtilis BU412 which could cause hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. The purification was executed by ion-exchange and size exclusion chromatography. The target band on SDS-PAGE was analyzed by mass spectrometry, and the...

journal_title：AMB Express

authors： Shen Y,Li J,Xiang J,Wang J,Yin K,Liu Q

更新日期：2019-07-27 00:00:00

abstract：:Rapidly developing industry raises concerns about the environmental impacts of nanoparticles, but the effects of inorganic nanoparticles on bacterial community in wastewater treatment remain unclear. The present research assessed the impact of cerium oxide nanoparticles (nCeO) on the microbiome of activated sludge sys...

journal_title：AMB Express

authors： Kamika I,Tekere M

更新日期：2017-12-01 00:00:00

abstract：:In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over a...

journal_title：AMB Express

authors： Ji JH,Zhou L,Mbadinga SM,Irfan M,Liu YF,Pan P,Qi ZZ,Chen J,Liu JF,Yang SZ,Gu JD,Mu BZ

更新日期：2020-02-01 00:00:00

abstract：:The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell b...

journal_title：AMB Express

authors： Werner A,Otte KL,Stahlhut G,Pöggeler S

更新日期：2020-12-21 00:00:00