Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris.

abstract：:The tissues of marine invertebrates are colonized by species-rich microbial communities. The dysbiosis of host's microbiota is tightly associated with the invertebrate diseases. Yesso scallop (Patinopecten yessoensis), one of the most important maricultured scallops in northern China, has recently suffered massive sum...

journal_title：AMB Express

authors： Yu Z,Liu C,Fu Q,Lu G,Han S,Wang L,Song L

更新日期：2019-09-14 00:00:00

abstract：:Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS m...

journal_title：AMB Express

authors： Liang J,Cheng T,Huang Y,Liu J

更新日期：2018-05-28 00:00:00

abstract：:Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of ...

journal_title：AMB Express

authors： Gao D,Chang K,Ding G,Wu H,Chen Y,Jia M,Liu X,Wang S,Jin Y,Pan H,Li H

更新日期：2019-05-24 00:00:00

abstract：:Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus...

journal_title：AMB Express

authors： Lu F,Huang Y,Zhang X,Wang Z

更新日期：2017-12-01 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：:Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

journal_title：AMB Express

authors： Fikry S,Khalil N,Salama O

更新日期：2019-03-26 00:00:00

abstract：:Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. Increasing interest in controlling water-borne pathogens in water resources evidenced by a large number of recent publications clearly attests to the need for studies that synthesize knowl...

journal_title：AMB Express

authors： Pandey PK,Kass PH,Soupir ML,Biswas S,Singh VP

更新日期：2014-06-28 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the prese...

journal_title：AMB Express

authors： Lee JH,Kim YG,Gwon G,Wood TK,Lee J

更新日期：2016-12-01 00:00:00

abstract：:High-performance liquid chromatography-mass spectrometry (LC-MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis ...

journal_title：AMB Express

authors： Gu F,Chen Y,Hong Y,Fang Y,Tan L

更新日期：2017-12-01 00:00:00

abstract：:The zygomycete fungus Lichtheimia ramosa H71D, isolated from sugarcane bagasse compost, was identified by applying phylogenetic analysis based on the DNA sequence of the Internal Transcribed Spacer (ITS), and subsequent secondary structure analysis of ITS2. L. ramosa H71D was able to grow over a wide range of temperat...

journal_title：AMB Express

authors： Alvarez-Zúñiga MT,Santiago-Hernández A,Rodríguez-Mendoza J,Campos JE,Pavón-Orozco P,Trejo-Estrada S,Hidalgo-Lara ME

更新日期：2017-11-02 00:00:00

abstract：:An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experiment...

journal_title：AMB Express

authors： Abdelrazek NA,Elkhatib WF,Raafat MM,Aboulwafa MM

更新日期：2020-04-15 00:00:00

abstract：:Phasins are amphiphilic proteins involved in the regulation of the number and size of polyhydroxybutyrate (PHB) granules. The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and energy source. By analyzing the genome, we identified six genes that cod...

journal_title：AMB Express

authors： Martínez-Martínez MLA,González-Pedrajo B,Dreyfus G,Soto-Urzúa L,Martínez-Morales LJ

更新日期：2019-09-25 00:00:00

abstract：:Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments. To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes ...

journal_title：AMB Express

authors： Lyu T,Liu G,Zhang H,Wang L,Zhou S,Dou H,Pang B,Sha W,Zhang H

更新日期：2018-08-02 00:00:00

abstract：: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

journal_title：AMB Express

authors： Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

更新日期：2012-04-25 00:00:00

abstract：:A novel strain of Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize medium chain-length polyhydroxyalkanoates (mcl-PHAs). P.putida LS46 was differentiated from other P.putida strains on the basis of cpn60 (UT). The complete genome of P.putida LS46 was sequenced and annotate...

journal_title：AMB Express

authors： Sharma PK,Fu J,Zhang X,Fristensky B,Sparling R,Levin DB

更新日期：2014-05-22 00:00:00

abstract：:High temperature causes ubiquitous environmental stress to microorganisms, but studies have not fully explained whether and to what extent heat shock would affect genome stability. Hence, this study explored heat-shock-induced genomic alterations in the yeast Saccharomyces cerevisiae. Using genetic screening systems a...

journal_title：AMB Express

authors： Shen L,Wang YT,Tang XX,Zhang K,Wang PM,Sui Y,Zheng DQ

更新日期：2020-08-17 00:00:00

abstract：:Bacterial contamination is one of the major challenges faced by yeast fermentation industries as the contaminating microorganisms produce lactic acid and acetic acid, which reduces the viability of yeast, and hence fermentation yields. The primary bacterial contaminants of yeast fermentations are lactic acid bacteria ...

journal_title：AMB Express

authors： Haris S,Fang C,Bastidas-Oyanedel JR,Prather KJ,Schmidt JE,Thomsen MH

更新日期：2018-08-06 00:00:00

abstract：:During industrial fermentation, Streptomyces clavuligerus F613-1 simultaneously produces primary product clavulanic acid (CA) and cephamycin C. The cephamycin C biosynthetic gene cluster and pathway have been basically elucidated and the CcaR positive regulator was found to control the cephamycin genes expression. How...

journal_title：AMB Express

authors： Fu J,Qin R,Zong G,Zhong C,Zhang P,Kang N,Qi X,Cao G

更新日期：2019-07-27 00:00:00

abstract：:The 70-kD heat shock proteins (Hsp70s) have been proved to be important for stress tolerance and protein folding and unfolding in almost all organisms. However, the functions of Hsp70s in mushroom are not well understood. In the present study, a hsp70 gene from Hypsizygus marmoreus, hmhsp70, was cloned and transferred...

journal_title：AMB Express

authors： Xu L,Gao J,Guo L,Yu H

更新日期：2020-01-18 00:00:00

abstract：:The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

journal_title：AMB Express

authors： Shonyela SM,Feng B,Yang W,Yang G,Wang C

更新日期：2020-04-17 00:00:00

abstract：:Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this ext...

journal_title：AMB Express

authors： Roberts DP,Selmer K,Lupitskyy R,Rice C,Buyer JS,Maul JE,Lakshman DK,DeSouza J

更新日期：2021-01-06 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：:The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in this biotransformation. The complete hydrolysis of 5-caffeoylquinic acid (5-CQA) into caffeic acid (CA) by L...

journal_title：AMB Express

authors： Bel-Rhlid R,Thapa D,Kraehenbuehl K,Hansen CE,Fischer L

更新日期：2013-05-21 00:00:00

abstract：:Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the alkali extraction process, xylanase and arabinofuranosidase are mainly used to hydrolyze xylooligosacc...

journal_title：AMB Express

authors： Zhang Y,Yang H,Yu X,Kong H,Chen J,Luo H,Bai Y,Yao B

更新日期：2019-01-29 00:00:00

abstract：:The utilization of inoculants containing Azospirillum is becoming more popular due to increasing reports of expressive gains in grain yields. However, incompatibility with pesticides used in seed treatments represents a main limitation for a successful inoculation. Therefore, in this study we searched for alternatives...

journal_title：AMB Express

authors： Fukami J,Nogueira MA,Araujo RS,Hungria M

更新日期：2016-03-01 00:00:00

abstract：:Mathematical models have been used from growth kinetic simulation to gen regulatory networks prediction for B. thuringiensis culture. However, this culture is a time dependent dynamic process where cells physiology suffers several changes depending on the changes in the cell environment. Therefore, through its culture...

journal_title：AMB Express

authors： Navarro-Mtz AK,Pérez-Guevara F

更新日期：2014-12-01 00:00:00

abstract：:Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase a...

journal_title：AMB Express

authors： Bayitse R,Hou X,Bjerre AB,Saalia FK

更新日期：2015-12-01 00:00:00

abstract：:Herbicides are important tools for weed control in modern agriculture. In the search for potential herbicidal natural products from fungal species, harzianum A and B were identified from the biofertilizer fungus, Trichoderma brevicompactum. In the phytotoxicity assays on the dicot species Brassica chinensis, harzianum...

journal_title：AMB Express

authors： Yin M,Fasoyin OE,Wang C,Yue Q,Zhang Y,Dun B,Xu Y,Zhang L

更新日期：2020-07-01 00:00:00

abstract：:Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L...

journal_title：AMB Express

authors： Kang TS,Korber DR,Tanaka T

更新日期：2013-01-31 00:00:00

abstract：:Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

journal_title：AMB Express

authors： Ng TW,Chan WL,Lai KM

更新日期：2017-12-01 00:00:00