Optimisation of enzymatic hydrolysis of cassava peel to produce fermentable sugars.

abstract：:Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

journal_title：AMB Express

authors： Fikry S,Khalil N,Salama O

更新日期：2019-03-26 00:00:00

abstract：:A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl2 treatment of the protoplasts and plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by a homologous promoter. During re...

journal_title：AMB Express

authors： Honda Y,Tanigawa E,Tsukihara T,Nguyen DX,Kawabe H,Sakatoku N,Watari J,Sato H,Yano S,Tachiki T,Irie T,Watanabe T,Watanabe T

更新日期：2019-06-24 00:00:00

abstract：:High temperature causes ubiquitous environmental stress to microorganisms, but studies have not fully explained whether and to what extent heat shock would affect genome stability. Hence, this study explored heat-shock-induced genomic alterations in the yeast Saccharomyces cerevisiae. Using genetic screening systems a...

journal_title：AMB Express

authors： Shen L,Wang YT,Tang XX,Zhang K,Wang PM,Sui Y,Zheng DQ

更新日期：2020-08-17 00:00:00

abstract：:Mathematical models have been used from growth kinetic simulation to gen regulatory networks prediction for B. thuringiensis culture. However, this culture is a time dependent dynamic process where cells physiology suffers several changes depending on the changes in the cell environment. Therefore, through its culture...

journal_title：AMB Express

authors： Navarro-Mtz AK,Pérez-Guevara F

更新日期：2014-12-01 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：:Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concent...

journal_title：AMB Express

authors： Chen D,Wang Y,Chen M,Fan P,Li G,Wang C

更新日期：2021-01-04 00:00:00

abstract：:Every year about 20% of the globally produced meat gets lost due to microbial spoilage. Nevertheless, the demand for processed meat is constantly rising and producers are searching for novel strategies to reduce microbial contaminations in their products. In the present study, we evaluated the applicability of alkylpy...

journal_title：AMB Express

authors： Schöck M,Liebminger S,Berg G,Cernava T

更新日期：2018-04-03 00:00:00

abstract：:The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees rev...

journal_title：AMB Express

authors： Oueslati M,Mulet M,Zouaoui M,Chandeysson C,Lalucat J,Hajlaoui MR,Berge O,García-Valdés E,Sadfi-Zouaoui N

更新日期：2020-11-01 00:00:00

abstract：:Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

journal_title：AMB Express

authors： Ng TW,Chan WL,Lai KM

更新日期：2017-12-01 00:00:00

abstract：:This study investigated the effects of the rumen fungus Piromyces sp. CN6 CGMCC 14449 as a silage additive on the fermentation quality, nutrient composition and in vitro digestibility of whole crop maize silage. Whole crop maize served as the silage material and was vacuum packed in polyethylene bags. Three ensiling t...

journal_title：AMB Express

authors： Wang D,Zhao C,Liu S,Zhang T,Yao J,Cao Y

更新日期：2019-07-29 00:00:00

abstract：: Vinasse, a highly polluting waste of the ethanol industry was utilized for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloferax mediterranei in shake-flasks. Following pre-treatment through adsorption on activated carbon, 25%-50% (v/v) pre-treated vinasse was utilized leading t...

journal_title：AMB Express

authors： Bhattacharyya A,Pramanik A,Maji SK,Haldar S,Mukhopadhyay UK,Mukherjee J

更新日期：2012-07-09 00:00:00

abstract：:Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS m...

journal_title：AMB Express

authors： Liang J,Cheng T,Huang Y,Liu J

更新日期：2018-05-28 00:00:00

abstract：:Vaccination is believed to be the most effective method for the prevention of infectious diseases. Thus it is imperative to develop cost effective and scalable process for the production of vaccines so as to make them affordable for mass use. In this study, performance of a novel disposable iCELLis fixed bed bioreacto...

journal_title：AMB Express

authors： Rajendran R,Lingala R,Vuppu SK,Bandi BO,Manickam E,Macherla SR,Dubois S,Havelange N,Maithal K

更新日期：2014-04-25 00:00:00

abstract：:Young barley leaf extract (YBL) contains beneficial substances such as fructans, minerals, and vitamins. The effects of YBL administration on the human colonic microbiota and its production of metabolites were evaluated using an in vitro model culture system. Fermentations were started by inoculating fecal samples fro...

journal_title：AMB Express

authors： Sasaki D,Sasaki K,Kadowaki Y,Aotsuka Y,Kondo A

更新日期：2019-11-13 00:00:00

abstract：:Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L...

journal_title：AMB Express

authors： Ece S,Lambertz C,Fischer R,Commandeur U

更新日期：2017-12-01 00:00:00

abstract：:Cyclophosphamide (CPA) represents a widely used anti-cancer prodrug that is converted by liver cytochrome P450 (CYP) enzymes into the primary metabolite 4-hydroxycyclophosphamide (4-OH-CPA), followed by non-enzymatic generation of the bioactive metabolites phosphoramide mustard and acrolein. The use of human drug meta...

journal_title：AMB Express

authors： Steinbrecht S,Kiebist J,König R,Thiessen M,Schmidtke KU,Kammerer S,Küpper JH,Scheibner K

更新日期：2020-07-18 00:00:00

abstract：:The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell b...

journal_title：AMB Express

authors： Werner A,Otte KL,Stahlhut G,Pöggeler S

更新日期：2020-12-21 00:00:00

abstract：:Thermomonospora curvata is a thermophilic actinomycete phylogenetically related to Thermobifida fusca that produces extracellular hydrolases capable of degrading synthetic polyesters. Analysis of the genome of T. curvata DSM43183 revealed two genes coding for putative polyester hydrolases Tcur1278 and Tcur0390 sharing...

journal_title：AMB Express

authors： Wei R,Oeser T,Then J,Kühn N,Barth M,Schmidt J,Zimmermann W

更新日期：2014-06-03 00:00:00

abstract：:In this study the aromatic nanocolloids (CANCs) are synthesized using the noble metal silver by using Citronella extract and confirmed through physio chemical analysis. The synthesised CANCs were evaluated for its antimicrobial activity and antibiofilm activity against pathogenic biofilm forming E. coli. In addition, ...

journal_title：AMB Express

authors： Soundhararajan R,Mohamed Sheik Meeran SAF,Prakash SP,Mohammad W,Kandasamy R,Srinivasan H

更新日期：2020-09-18 00:00:00

abstract：:Disentangling the interactions between cyanobacteria and associated bacterial community is important for understanding the mechanisms that mediate the formation of cyanobacterial blooms in freshwater ecosystems. Despite the fact that a metagenomic approach enables researchers to profile the structure of microbial comm...

journal_title：AMB Express

authors： Ren M,Zhang G,Ye Z,Qiao Z,Xie M,Lin Y,Li T,Zhao J

更新日期：2017-11-02 00:00:00

abstract：:Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella living...

journal_title：AMB Express

authors： Tajima T,Fuki K,Kataoka N,Kudou D,Nakashimada Y,Kato J

更新日期：2013-12-05 00:00:00

abstract：:PCV3 capsid protein (Cap) is an important antigen for diagnosis and vaccine development. To achieve high-level expression of recombinant PCV3 Cap in Escherichia coli (E. coli), the gene of wild-type entire Cap (wt-eCap) was amplified from clinical samples, and three optimized entire Cap (opti-eCap) and one optimized C...

journal_title：AMB Express

authors： Wang Y,Wang G,Duan WT,Sun MX,Wang MH,Wang SH,Cai XH,Tu YB

更新日期：2020-01-07 00:00:00

abstract：:The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is comparatively simple, well characterized and can be manipulated easily...

journal_title：AMB Express

authors： Kangwa M,Yelemane V,Polat AN,Gorrepati KD,Grasselli M,Fernández-Lahore M

更新日期：2015-12-01 00:00:00

abstract：:Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a swine respiratory disease with an important impact around the world either as a single infection or part of the porcine respiratory disease complex. The data of interaction between hosts and pathogens has becoming more crucial for exploration...

journal_title：AMB Express

authors： Zhu R,Bao C,Liu B,Xiao J,Sun C,Feng X,Langford PR,Li Y,Lei L

更新日期：2020-07-06 00:00:00

abstract：:The Editor-in-Chief has retracted this article (Wang et al. 2018) because the authors do not have ownership of the data they report. An investigation by the Commonwealth Scientific and Industrial Research Organisation (CSIRO) has concluded that the data reported in this article are the sole property of the CSIRO. Ming...

journal_title：AMB Express

authors： Wang D,Liu Y,Lv D,Hu X,Zhong Q,Zhao Y,Wu M

更新日期：2019-04-19 00:00:00

abstract：:MicroRNAs (miRs) have shown tremendous potential to act as therapeutic targets for cancer treatment. In this context, the present study was designed to investigate the potential of miR-143 in the treatment of breast cancer. Results showed that miR-143 to be significantly (P < 0.05) downregulated in breast cancer tissu...

journal_title：AMB Express

authors： Du Y,Zhang J,Meng Y,Huang M,Yan W,Wu Z

更新日期：2020-07-31 00:00:00

abstract：:Chlorothalonil, a non-systemic and broad-spectrum fungicide, is widely used to control the pathogens of agricultural plants. Although microbial degradation of chlorothalonil is known, we know little about the colonization and degradation capacity of these microbes in the natural and semi-natural soil environments. The...

journal_title：AMB Express

authors： Zhang Q,Saleem M,Wang C

更新日期：2017-12-23 00:00:00

abstract：:Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

journal_title：AMB Express

authors： Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

更新日期：2020-02-25 00:00:00

abstract：:Docosahexaenoic acid (DHA) enriched in brain can yield many important degradation products after the attack of hydroxyl radicals, which is known to serve as a nutraceutical and neuroprotective effects. Oxidative stress is a commonly observed feature of Alzheimer's disease (AD). Therefore, uniformly radiolabeled DHA pl...

journal_title：AMB Express

authors： Song P,Kuryatov A,Axelsen PH

更新日期：2020-03-11 00:00:00

abstract：:Crude enzyme from Geotrichum candidum S12 exhibited high activity towards hexanol at pH 4.0, distinguishing it from currently known enzymes. To identify the dominant enzyme contributing to this activity, the crude enzyme extract was separated into different fractions by ammonium sulfate precipitation, MonoQ anion-exch...

journal_title：AMB Express

authors： Zhu J,Lu K,Xu X,Wang X,Shi J

更新日期：2017-12-01 00:00:00