Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora.

abstract：:Bacterial contamination is one of the major challenges faced by yeast fermentation industries as the contaminating microorganisms produce lactic acid and acetic acid, which reduces the viability of yeast, and hence fermentation yields. The primary bacterial contaminants of yeast fermentations are lactic acid bacteria ...

journal_title：AMB Express

authors： Haris S,Fang C,Bastidas-Oyanedel JR,Prather KJ,Schmidt JE,Thomsen MH

更新日期：2018-08-06 00:00:00

abstract：:Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade ...

journal_title：AMB Express

authors： Liu Z,Dang K,Li C,Gao J,Wang H,Gao Y,Zhao B,Fan P,Qian A

更新日期：2020-10-31 00:00:00

abstract：:Cellulose degrading enzymes usually have a two-domain structure consisting of a catalytic domain and a non-catalytic carbohydrate-binding module. Although it is well known the importance of those modules in cell wall degrading process, their function is not yet fully understood. Here, we analyze the cellulose-hydrolys...

journal_title：AMB Express

authors： Mello BL,Polikarpov I

更新日期：2014-04-25 00:00:00

abstract：:The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

journal_title：AMB Express

authors： Shonyela SM,Feng B,Yang W,Yang G,Wang C

更新日期：2020-04-17 00:00:00

abstract：:Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

journal_title：AMB Express

authors： Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

更新日期：2020-02-25 00:00:00

abstract：:Fusicocca-2,10(14)-diene (FCdiene) is a diterpene which is interesting as a precursor of the anti-cancer drug fusicoccin A and therefore for pharmaceutical applications. Production of FCdiene using a genetically modified Saccharomyces cerevisiae has been previously demonstrated with batch cultivations with a product c...

journal_title：AMB Express

authors： Halka LM,Nowacki C,Kleinschmidt A,Koenen K,Wichmann R

更新日期：2018-08-22 00:00:00

abstract：:During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the S...

journal_title：AMB Express

authors： El-Rotail AAMM,Zhang L,Li Y,Liu SP,Shi GY

更新日期：2017-12-01 00:00:00

abstract：:Rapidly developing industry raises concerns about the environmental impacts of nanoparticles, but the effects of inorganic nanoparticles on bacterial community in wastewater treatment remain unclear. The present research assessed the impact of cerium oxide nanoparticles (nCeO) on the microbiome of activated sludge sys...

journal_title：AMB Express

authors： Kamika I,Tekere M

更新日期：2017-12-01 00:00:00

abstract：:A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase...

journal_title：AMB Express

authors： Knudsen JD,Carlquist M,Gorwa-Grauslund M

更新日期：2014-10-30 00:00:00

abstract：:Here, we report a novel protein elicitor from Bacillus subtilis BU412 which could cause hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. The purification was executed by ion-exchange and size exclusion chromatography. The target band on SDS-PAGE was analyzed by mass spectrometry, and the...

journal_title：AMB Express

authors： Shen Y,Li J,Xiang J,Wang J,Yin K,Liu Q

更新日期：2019-07-27 00:00:00

abstract：: Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay for measuring glucose uptake in skeletal muscle cells was established. During the screening studies to discov...

journal_title：AMB Express

authors： Kulkarni-Almeida AA,Brahma MK,Padmanabhan P,Mishra PD,Parab RR,Gaikwad NV,Thakkar CS,Tokdar P,Ranadive PV,Nair AS,Damre AA,Bahirat UA,Deshmukh NJ,Doshi LS,Dixit AV,George SD,Vishwakarma RA,Nemmani KV,Mahajan GB

更新日期：2011-11-21 00:00:00

abstract：:The tissues of marine invertebrates are colonized by species-rich microbial communities. The dysbiosis of host's microbiota is tightly associated with the invertebrate diseases. Yesso scallop (Patinopecten yessoensis), one of the most important maricultured scallops in northern China, has recently suffered massive sum...

journal_title：AMB Express

authors： Yu Z,Liu C,Fu Q,Lu G,Han S,Wang L,Song L

更新日期：2019-09-14 00:00:00

abstract：:The basidiospores of most Agaricomycetes are ballistospores. They are propelled off from their basidia at maturity when Buller's drop develops at high humidity at the hilar spore appendix and fuses with a liquid film formed on the adaxial side of the spore. Spores are catapulted into the free air space between hymenia...

journal_title：AMB Express

authors： Lakkireddy K,Kües U

更新日期：2017-12-01 00:00:00

abstract：:Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase a...

journal_title：AMB Express

authors： Bayitse R,Hou X,Bjerre AB,Saalia FK

更新日期：2015-12-01 00:00:00

abstract：:Clean coal technologies (e.g. coal biosolubilization) are of essential value, especially in Europe, where coal is the national wealth and other energy sources like crude oil are not available. Fusarium oxysporum LOCK 1134, the strain isolated from brown coal, efficiently biosolubilizes lignite. The obtained liquefied ...

journal_title：AMB Express

authors： Kwiatos N,Jędrzejczak-Krzepkowska M,Strzelecki B,Bielecki S

更新日期：2018-08-22 00:00:00

abstract：:The study was carried out to investigate the effect of dietary selenium (Se) and vitamin E (VE) supplementation on mRNA level of heat shock proteins, selenoproteins, and antioxidant enzyme activities in the breast meat of broilers under summer heat stress conditions. A total of 200 male broilers (Ross 308) of 1 day ag...

journal_title：AMB Express

authors： Kumbhar S,Khan AZ,Parveen F,Nizamani ZA,Siyal FA,El-Hack MEA,Gan F,Liu Y,Hamid M,Nido SA,Huang K

更新日期：2018-07-10 00:00:00

abstract：:Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5...

journal_title：AMB Express

authors： Duarte JC,Valença GP,Moran PJ,Rodrigues JA

更新日期：2015-02-20 00:00:00

abstract：:Docosahexaenoic acid (DHA) enriched in brain can yield many important degradation products after the attack of hydroxyl radicals, which is known to serve as a nutraceutical and neuroprotective effects. Oxidative stress is a commonly observed feature of Alzheimer's disease (AD). Therefore, uniformly radiolabeled DHA pl...

journal_title：AMB Express

authors： Song P,Kuryatov A,Axelsen PH

更新日期：2020-03-11 00:00:00

abstract：:The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees rev...

journal_title：AMB Express

authors： Oueslati M,Mulet M,Zouaoui M,Chandeysson C,Lalucat J,Hajlaoui MR,Berge O,García-Valdés E,Sadfi-Zouaoui N

更新日期：2020-11-01 00:00:00

abstract：:Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this ext...

journal_title：AMB Express

authors： Roberts DP,Selmer K,Lupitskyy R,Rice C,Buyer JS,Maul JE,Lakshman DK,DeSouza J

更新日期：2021-01-06 00:00:00

abstract：:Crude enzyme from Geotrichum candidum S12 exhibited high activity towards hexanol at pH 4.0, distinguishing it from currently known enzymes. To identify the dominant enzyme contributing to this activity, the crude enzyme extract was separated into different fractions by ammonium sulfate precipitation, MonoQ anion-exch...

journal_title：AMB Express

authors： Zhu J,Lu K,Xu X,Wang X,Shi J

更新日期：2017-12-01 00:00:00

abstract：:The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in this biotransformation. The complete hydrolysis of 5-caffeoylquinic acid (5-CQA) into caffeic acid (CA) by L...

journal_title：AMB Express

authors： Bel-Rhlid R,Thapa D,Kraehenbuehl K,Hansen CE,Fischer L

更新日期：2013-05-21 00:00:00

abstract：:Gene therapy and viral vaccine are becoming attractive therapeutic options for the treatment of different malignant diseases. Viral vector productions are often using static culture vessels and small volume stainless steel bioreactors (SSB). However, the yield of each vessel can be relatively low and multiple vessels ...

journal_title：AMB Express

authors： Yang J,Guertin P,Jia G,Lv Z,Yang H,Ju D

更新日期：2019-05-24 00:00:00

abstract：:Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

journal_title：AMB Express

authors： Fikry S,Khalil N,Salama O

更新日期：2019-03-26 00:00:00

abstract：:Sponges are primitive metazoans that are known to harbour diverse and abundant microbes. All over the world attempts are being made to exploit these microbes for their biotechnological potential to produce, bioactive compounds and antimicrobial peptides. However, the majority of the studies are focussed on the marine ...

journal_title：AMB Express

authors： Gaikwad S,Shouche YS,Gade WN

更新日期：2016-12-01 00:00:00

abstract：: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

journal_title：AMB Express

authors： Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

更新日期：2012-04-25 00:00:00

abstract：:Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concent...

journal_title：AMB Express

authors： Chen D,Wang Y,Chen M,Fan P,Li G,Wang C

更新日期：2021-01-04 00:00:00

abstract：:Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box-Behnken design with three factors, the glucose and sodium acetate conce...

journal_title：AMB Express

authors： Silva HR,Prete CEC,Zambrano F,de Mello VH,Tischer CA,Andrade DS

更新日期：2016-03-01 00:00:00

abstract：:Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natur...

journal_title：AMB Express

authors： Roth T,Beer B,Pick A,Sieber V

更新日期：2017-12-01 00:00:00

abstract：:The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...

journal_title：AMB Express

authors： Ibrahim MHA,Lebbe L,Willems A,Steinbüchel A

更新日期：2016-12-01 00:00:00