Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia.

Abstract:

:The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.

journal_name

AMB Express

journal_title

AMB Express

authors

Oueslati M,Mulet M,Zouaoui M,Chandeysson C,Lalucat J,Hajlaoui MR,Berge O,García-Valdés E,Sadfi-Zouaoui N

doi

10.1186/s13568-020-01134-z

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

198

issue

1

issn

2191-0855

pii

10.1186/s13568-020-01134-z

journal_volume

10

pub_type

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