A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities.

Abstract:

:Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify active ingredient from Bacillus amyloliquefaciens GN59 and study its antibacterial activity. A novel compound was isolated from GN59 by column chromatography on silica gel and HPLC purification. The chemical structure was identified as α-D-glucopyranosyl-(1 → 1')-3'-amino-3'-deoxy-β-D-glucopyranoside (a,β-3-trehalosamine) on the basis of spectroscopic analysis. This is the first report about a,β-3-trehalosamine isolated from biological resources on an antibiotic activity against pathogenic bacterium. The 3'-neotrehalosamine displayed antibacterial activity across a broad spectrum of microorganisms, including different gram-positive and gram-negative bacteria, with minimal inhibitory concentration (MIC) values ranging from 0.5 to 0.7 mg/mL. The results indicated that the 3'-neotrehalosamine from GN59 might be a potential candidate for bactericide.

journal_name

AMB Express

journal_title

AMB Express

authors

Wang Y,Zhao B,Liu Y,Mao L,Zhang X,Meng W,Liu K,Chu J

doi

10.1186/s13568-019-0943-x

subject

Has Abstract

pub_date

2020-01-14 00:00:00

pages

6

issue

1

issn

2191-0855

pii

10.1186/s13568-019-0943-x

journal_volume

10

pub_type

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