Assessment of packed bed bioreactor systems in the production of viral vaccines.

abstract：:Cordyceps militaris is a type of entomogenous fungi and has been widely used as a medicinal fungus in Asia. Cordycepin produced by C. militaris has also been found to protect the liver. Moreover, deep ocean water (DOW) was proven to increase the functional compounds of functional fungi-fermented products. However, the...

journal_title：AMB Express

authors： Hung YP,Wang JJ,Wei BL,Lee CL

更新日期：2015-12-01 00:00:00

abstract：:Young barley leaf extract (YBL) contains beneficial substances such as fructans, minerals, and vitamins. The effects of YBL administration on the human colonic microbiota and its production of metabolites were evaluated using an in vitro model culture system. Fermentations were started by inoculating fecal samples fro...

journal_title：AMB Express

authors： Sasaki D,Sasaki K,Kadowaki Y,Aotsuka Y,Kondo A

更新日期：2019-11-13 00:00:00

abstract：:During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the S...

journal_title：AMB Express

authors： El-Rotail AAMM,Zhang L,Li Y,Liu SP,Shi GY

更新日期：2017-12-01 00:00:00

abstract：:Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5...

journal_title：AMB Express

authors： Duarte JC,Valença GP,Moran PJ,Rodrigues JA

更新日期：2015-02-20 00:00:00

abstract：:Increasing evidences suggest that intestinal microbiota balance closely correlated with host's health status could affected by external environment. Integrated crayfish-rice cultivation model is a highly efficient artificial ecosystem widely practiced in subtropical China. Less information is available to estimate the...

journal_title：AMB Express

authors： Shui Y,Guan ZB,Liu GF,Fan LM

更新日期：2020-01-14 00:00:00

abstract：: Vinasse, a highly polluting waste of the ethanol industry was utilized for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloferax mediterranei in shake-flasks. Following pre-treatment through adsorption on activated carbon, 25%-50% (v/v) pre-treated vinasse was utilized leading t...

journal_title：AMB Express

authors： Bhattacharyya A,Pramanik A,Maji SK,Haldar S,Mukhopadhyay UK,Mukherjee J

更新日期：2012-07-09 00:00:00

abstract：:This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine. In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccha...

journal_title：AMB Express

authors： Benito Á,Calderón F,Benito S

更新日期：2019-02-02 00:00:00

abstract：:Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a swine respiratory disease with an important impact around the world either as a single infection or part of the porcine respiratory disease complex. The data of interaction between hosts and pathogens has becoming more crucial for exploration...

journal_title：AMB Express

authors： Zhu R,Bao C,Liu B,Xiao J,Sun C,Feng X,Langford PR,Li Y,Lei L

更新日期：2020-07-06 00:00:00

abstract：:Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concent...

journal_title：AMB Express

authors： Chen D,Wang Y,Chen M,Fan P,Li G,Wang C

更新日期：2021-01-04 00:00:00

abstract：:The effects of inlet VOCs (Volatile Organic Compounds) shifts on microbial community structure in a biofiltration system were investigated. A lab-scale biofilter was set up to treat eight VOCs sequentially. Short declines in removal efficiency appeared after VOCs shifts and then later recovered. The number of OTUs in ...

journal_title：AMB Express

authors： Lu L,Wang G,Yeung M,Xi J,Hu HY

更新日期：2018-10-03 00:00:00

abstract：:Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitiv...

journal_title：AMB Express

authors： Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZL

更新日期：2020-12-11 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：:Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. Lipophilic dyes such as Nile red and BODIPY have been used to quantify intracellular PHB, but their uses have often been limited in...

journal_title：AMB Express

authors： Choi JE,Na HY,Yang TH,Rhee SK,Song JK

更新日期：2015-12-01 00:00:00

abstract：:Lactobacilli, as the largest group of lactic acid bacteria, produce large amounts of antimicrobial metabolites such as organic acids, fatty acids, ammonia, hydrogen peroxide, diacetyl and bacteriocin, which inhibit the growth of pathogenic bacteria and increase shelf life of food. The aim of this study was to identify...

journal_title：AMB Express

authors： Azizi F,Habibi Najafi MB,Edalatian Dovom MR

更新日期：2017-09-18 00:00:00

abstract：:The 70-kD heat shock proteins (Hsp70s) have been proved to be important for stress tolerance and protein folding and unfolding in almost all organisms. However, the functions of Hsp70s in mushroom are not well understood. In the present study, a hsp70 gene from Hypsizygus marmoreus, hmhsp70, was cloned and transferred...

journal_title：AMB Express

authors： Xu L,Gao J,Guo L,Yu H

更新日期：2020-01-18 00:00:00

abstract：:Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and fe...

journal_title：AMB Express

authors： Li XJ,Wang M,Xue Y,Duan D,Li C,Han X,Wang K,Qiao R,Li XL

更新日期：2020-10-29 00:00:00

abstract：:Plant extracts serve as reducing and coating agents and are, therefore, commonly employed for the generation of silver (Ag) nanoparticles (NPs). Plant extract mediated synthesis of Ag NPs is a green, environmentally friendly and cost-effective technique which offers a new and potential alternative to chemically synthe...

journal_title：AMB Express

authors： Hambardzumyan S,Sahakyan N,Petrosyan M,Nasim MJ,Jacob C,Trchounian A

更新日期：2020-09-05 00:00:00

abstract：:The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

journal_title：AMB Express

authors： Shonyela SM,Feng B,Yang W,Yang G,Wang C

更新日期：2020-04-17 00:00:00

abstract：:The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs functi...

journal_title：AMB Express

authors： Aghajanyan A,Gabrielyan L,Schubert R,Trchounian A

更新日期：2020-04-07 00:00:00

abstract：:In this paper, we demonstrate that the antimicrobial activity of L. plantarum PBS067 strain against antagonist microorganisms was mediated by the production of a bacteriocin-like compound secreted at the stationary phase of the growth. The novel bacteriocin-like compound, designed plantaricin P1053, was identified by ...

journal_title：AMB Express

authors： De Giani A,Bovio F,Forcella M,Fusi P,Sello G,Di Gennaro P

更新日期：2019-06-17 00:00:00

abstract：:The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is comparatively simple, well characterized and can be manipulated easily...

journal_title：AMB Express

authors： Kangwa M,Yelemane V,Polat AN,Gorrepati KD,Grasselli M,Fernández-Lahore M

更新日期：2015-12-01 00:00:00

abstract：:Cellulose degrading enzymes usually have a two-domain structure consisting of a catalytic domain and a non-catalytic carbohydrate-binding module. Although it is well known the importance of those modules in cell wall degrading process, their function is not yet fully understood. Here, we analyze the cellulose-hydrolys...

journal_title：AMB Express

authors： Mello BL,Polikarpov I

更新日期：2014-04-25 00:00:00

abstract：:This study investigated the effects of the rumen fungus Piromyces sp. CN6 CGMCC 14449 as a silage additive on the fermentation quality, nutrient composition and in vitro digestibility of whole crop maize silage. Whole crop maize served as the silage material and was vacuum packed in polyethylene bags. Three ensiling t...

journal_title：AMB Express

authors： Wang D,Zhao C,Liu S,Zhang T,Yao J,Cao Y

更新日期：2019-07-29 00:00:00

abstract：:Crude enzyme from Geotrichum candidum S12 exhibited high activity towards hexanol at pH 4.0, distinguishing it from currently known enzymes. To identify the dominant enzyme contributing to this activity, the crude enzyme extract was separated into different fractions by ammonium sulfate precipitation, MonoQ anion-exch...

journal_title：AMB Express

authors： Zhu J,Lu K,Xu X,Wang X,Shi J

更新日期：2017-12-01 00:00:00

abstract：:Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L...

journal_title：AMB Express

authors： Ece S,Lambertz C,Fischer R,Commandeur U

更新日期：2017-12-01 00:00:00

abstract：:MicroRNAs (miRs) have shown tremendous potential to act as therapeutic targets for cancer treatment. In this context, the present study was designed to investigate the potential of miR-143 in the treatment of breast cancer. Results showed that miR-143 to be significantly (P < 0.05) downregulated in breast cancer tissu...

journal_title：AMB Express

authors： Du Y,Zhang J,Meng Y,Huang M,Yan W,Wu Z

更新日期：2020-07-31 00:00:00

abstract：:The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broa...

journal_title：AMB Express

authors： Ao XL,Yu X,Wu DT,Li C,Zhang T,Liu SL,Chen SJ,He L,Zhou K,Zou LK

更新日期：2018-06-12 00:00:00

abstract：: Effective utilization of photosynthetic microorganisms as potential biocatalysts is favorable for the production of useful biomaterials and the reduction of atmospheric CO2. For example, biocatalytic transformations are used in the synthesis of optically active alcohols. We previously found that ketone reduction in c...

journal_title：AMB Express

authors： Yamanaka R,Nakamura K,Murakami A

更新日期：2011-09-01 00:00:00

abstract：:Mycolata form a group of Gram-positive bacteria with unique cell envelope structures that are known for their high tolerance against antibiotics and both aromatic and aliphatic hydrocarbons. An important part of the unique surface structure of the mycolata is the presence of long chain α-alkyl-β-hydroxy fatty acids, t...

journal_title：AMB Express

authors： de Carvalho CCCR,Fischer MA,Kirsten S,Würz B,Wick LY,Heipieper HJ

更新日期：2016-12-01 00:00:00

abstract：:The tissues of marine invertebrates are colonized by species-rich microbial communities. The dysbiosis of host's microbiota is tightly associated with the invertebrate diseases. Yesso scallop (Patinopecten yessoensis), one of the most important maricultured scallops in northern China, has recently suffered massive sum...

journal_title：AMB Express

authors： Yu Z,Liu C,Fu Q,Lu G,Han S,Wang L,Song L

更新日期：2019-09-14 00:00:00