Effect of the salts of deep ocean water on the production of cordycepin and adenosine of Cordyceps militaris-fermented product.

abstract：:Crude enzyme from Geotrichum candidum S12 exhibited high activity towards hexanol at pH 4.0, distinguishing it from currently known enzymes. To identify the dominant enzyme contributing to this activity, the crude enzyme extract was separated into different fractions by ammonium sulfate precipitation, MonoQ anion-exch...

journal_title：AMB Express

authors： Zhu J,Lu K,Xu X,Wang X,Shi J

更新日期：2017-12-01 00:00:00

abstract：:Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli ar...

journal_title：AMB Express

authors： Mozejko-Ciesielska J,Dabrowska D,Szalewska-Palasz A,Ciesielski S

更新日期：2017-12-01 00:00:00

abstract：:The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees rev...

journal_title：AMB Express

authors： Oueslati M,Mulet M,Zouaoui M,Chandeysson C,Lalucat J,Hajlaoui MR,Berge O,García-Valdés E,Sadfi-Zouaoui N

更新日期：2020-11-01 00:00:00

abstract：:The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes...

journal_title：AMB Express

authors： Bartolo-Aguilar Y,Dendooven L,Chávez-Cabrera C,Flores-Cotera LB,Hidalgo-Lara ME,Villa-Tanaca L,Marsch R

更新日期：2017-12-01 00:00:00

abstract：:Biological technologies for recycling rare metals, which are essential for high-tech products, have attracted much attention because they could prove to be more environmentally friendly and energy-saving than other methods. We have developed biological recycling technologies by cell surface engineering for the selecti...

journal_title：AMB Express

authors： Ito R,Kuroda K,Hashimoto H,Ueda M

更新日期：2016-12-01 00:00:00

abstract：:PCV3 capsid protein (Cap) is an important antigen for diagnosis and vaccine development. To achieve high-level expression of recombinant PCV3 Cap in Escherichia coli (E. coli), the gene of wild-type entire Cap (wt-eCap) was amplified from clinical samples, and three optimized entire Cap (opti-eCap) and one optimized C...

journal_title：AMB Express

authors： Wang Y,Wang G,Duan WT,Sun MX,Wang MH,Wang SH,Cai XH,Tu YB

更新日期：2020-01-07 00:00:00

abstract：:The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternativ...

journal_title：AMB Express

authors： da Cruz Ramos GF,Ramos PL,Passarini MRZ,Vieira Silveira MA,Okamoto DN,de Oliveira LCG,Zezzo LV,Marem A,Santos Rocha RC,da Cruz JB,Juliano L,de Vasconcellos SP

更新日期：2016-03-01 00:00:00

abstract：:Bacterial contamination is one of the major challenges faced by yeast fermentation industries as the contaminating microorganisms produce lactic acid and acetic acid, which reduces the viability of yeast, and hence fermentation yields. The primary bacterial contaminants of yeast fermentations are lactic acid bacteria ...

journal_title：AMB Express

authors： Haris S,Fang C,Bastidas-Oyanedel JR,Prather KJ,Schmidt JE,Thomsen MH

更新日期：2018-08-06 00:00:00

abstract：:Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella living...

journal_title：AMB Express

authors： Tajima T,Fuki K,Kataoka N,Kudou D,Nakashimada Y,Kato J

更新日期：2013-12-05 00:00:00

abstract：:Calebin-A is a curcuminoid compound reported to be present in Curcuma longa rhizome. The current study was aimed to isolate and characterize calebin-A from Curcuma caesia rhizome and its production through biotransformation approach using endophytic fungus. C. caesia rhizomes of different ages were subjected to analys...

journal_title：AMB Express

authors： Majeed A,Majeed M,Thajuddin N,Arumugam S,Ali F,Beede K,Adams SJ,Gnanamani M

更新日期：2019-05-29 00:00:00

abstract：:In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over a...

journal_title：AMB Express

authors： Ji JH,Zhou L,Mbadinga SM,Irfan M,Liu YF,Pan P,Qi ZZ,Chen J,Liu JF,Yang SZ,Gu JD,Mu BZ

更新日期：2020-02-01 00:00:00

abstract：:Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natur...

journal_title：AMB Express

authors： Roth T,Beer B,Pick A,Sieber V

更新日期：2017-12-01 00:00:00

abstract：:The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in this biotransformation. The complete hydrolysis of 5-caffeoylquinic acid (5-CQA) into caffeic acid (CA) by L...

journal_title：AMB Express

authors： Bel-Rhlid R,Thapa D,Kraehenbuehl K,Hansen CE,Fischer L

更新日期：2013-05-21 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：:Mathematical models have been used from growth kinetic simulation to gen regulatory networks prediction for B. thuringiensis culture. However, this culture is a time dependent dynamic process where cells physiology suffers several changes depending on the changes in the cell environment. Therefore, through its culture...

journal_title：AMB Express

authors： Navarro-Mtz AK,Pérez-Guevara F

更新日期：2014-12-01 00:00:00

abstract：:Chlorothalonil, a non-systemic and broad-spectrum fungicide, is widely used to control the pathogens of agricultural plants. Although microbial degradation of chlorothalonil is known, we know little about the colonization and degradation capacity of these microbes in the natural and semi-natural soil environments. The...

journal_title：AMB Express

authors： Zhang Q,Saleem M,Wang C

更新日期：2017-12-23 00:00:00

abstract：:Lactobacilli, as the largest group of lactic acid bacteria, produce large amounts of antimicrobial metabolites such as organic acids, fatty acids, ammonia, hydrogen peroxide, diacetyl and bacteriocin, which inhibit the growth of pathogenic bacteria and increase shelf life of food. The aim of this study was to identify...

journal_title：AMB Express

authors： Azizi F,Habibi Najafi MB,Edalatian Dovom MR

更新日期：2017-09-18 00:00:00

abstract：:Denitrifying anaerobic methane oxidation (damo) bioprocesses can remove nitrate using methane as the electron donor, which gains great concern due to the current stringent discharge standard of nitrogen in wastewater treatment plants. To obtain an engineering acceptable nitrogen removal rate (NRR) and demonstrate the ...

journal_title：AMB Express

authors： Li W,Lu P,Chai F,Zhang L,Han X,Zhang D

更新日期：2018-06-30 00:00:00

abstract：:Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade ...

journal_title：AMB Express

authors： Liu Z,Dang K,Li C,Gao J,Wang H,Gao Y,Zhao B,Fan P,Qian A

更新日期：2020-10-31 00:00:00

abstract：:Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L...

journal_title：AMB Express

authors： Ece S,Lambertz C,Fischer R,Commandeur U

更新日期：2017-12-01 00:00:00

abstract：:Sponges are primitive metazoans that are known to harbour diverse and abundant microbes. All over the world attempts are being made to exploit these microbes for their biotechnological potential to produce, bioactive compounds and antimicrobial peptides. However, the majority of the studies are focussed on the marine ...

journal_title：AMB Express

authors： Gaikwad S,Shouche YS,Gade WN

更新日期：2016-12-01 00:00:00

abstract：:The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broa...

journal_title：AMB Express

authors： Ao XL,Yu X,Wu DT,Li C,Zhang T,Liu SL,Chen SJ,He L,Zhou K,Zou LK

更新日期：2018-06-12 00:00:00

abstract：:Geniposide is an iridoid glycoside, which is abundant in Gardeniae Fructus. Despite the various pharmaceutical effects of geniposide on a human body, its hydrolysis into a smaller molecule, genipin, by β-glucosidase produced by bacteria in the intestines is particularly important to improve geniposide uptake into the ...

journal_title：AMB Express

authors： Kim YS,Lee CJ,Ma JY

更新日期：2017-12-01 00:00:00

abstract：:During industrial fermentation, Streptomyces clavuligerus F613-1 simultaneously produces primary product clavulanic acid (CA) and cephamycin C. The cephamycin C biosynthetic gene cluster and pathway have been basically elucidated and the CcaR positive regulator was found to control the cephamycin genes expression. How...

journal_title：AMB Express

authors： Fu J,Qin R,Zong G,Zhong C,Zhang P,Kang N,Qi X,Cao G

更新日期：2019-07-27 00:00:00

abstract：:Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

journal_title：AMB Express

authors： Ng TW,Chan WL,Lai KM

更新日期：2017-12-01 00:00:00

abstract：: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

journal_title：AMB Express

authors： Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

更新日期：2012-04-25 00:00:00

abstract：:The basidiospores of most Agaricomycetes are ballistospores. They are propelled off from their basidia at maturity when Buller's drop develops at high humidity at the hilar spore appendix and fuses with a liquid film formed on the adaxial side of the spore. Spores are catapulted into the free air space between hymenia...

journal_title：AMB Express

authors： Lakkireddy K,Kües U

更新日期：2017-12-01 00:00:00

abstract：:Poly-gamma-glutamic acid (PGA) is a promising bio-based polymer that shares many functions with poly (acrylic acid) and its derivatives. Thus, technologies for efficient production and molecular size control of PGA are required to expand the application of this useful biopolymer. In Bacillus strains, PGA is synthesize...

journal_title：AMB Express

authors： Sawada K,Araki H,Takimura Y,Masuda K,Kageyama Y,Ozaki K,Hagihara H

更新日期：2018-07-03 00:00:00

abstract：:Ginsenoside compound K has been used as a key nutritional and cosmetic component because of its anti-fatigue and skin anti-aging effects. β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is known as the most efficient enzyme for compound K production. The hydrolytic pathway from ginsenoside Rb1 to compound K via Rd...

journal_title：AMB Express

authors： Shin KC,Choi HY,Seo MJ,Oh DK

更新日期：2017-10-04 00:00:00

abstract：:Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS m...

journal_title：AMB Express

authors： Liang J,Cheng T,Huang Y,Liu J

更新日期：2018-05-28 00:00:00