Bioconversion of curcumin into calebin-A by the endophytic fungus Ovatospora brasiliensis EPE-10 MTCC 25236 associated with Curcuma caesia.

Abstract:

:Calebin-A is a curcuminoid compound reported to be present in Curcuma longa rhizome. The current study was aimed to isolate and characterize calebin-A from Curcuma caesia rhizome and its production through biotransformation approach using endophytic fungus. C. caesia rhizomes of different ages were subjected to analysis in order to investigate the age at which maximum calebin-A content is present. HP-TLC profiles, HPLC retention times and mass spectrometry detector confirmed the occurrence of calebin-A in C. caesia rhizomes of 12 to 14 months of age but not in rhizomes younger to 12 months. Furthermore, an endophytic fungus strain, EPE-10 that was isolated from the medicinal plant C. caesia was identified as Ovatospora brasiliensis based on morphological and molecular characteristics. This strain O. brasiliensis was deposited to the culture collected centre, MTCC Chandigarh, India under the Budapest treaty and was designated with the Accession Number MTCC 25236. Biotransformation process was carried out at 37 ± 0.5 °C with shaking for 7 days after addition of 0.01% w/v curcumin. Extraction of biotransformed products was done by following partition method and the extracts obtained were analyzed using HPTLC, HPLC and LCMS. The data of the study suggested that O. brasiliensis MTCC 25236 was found to convert curcumin to calebin-A in a time dependant manner with optimum conversion at 48 h. Furthermore, O. brasiliensis MTCC 25236 was found to be positive for the Baeyer-Villiger monooxygenase (BVMOs) enzyme activity which could possibly be the mechanism of this bioconversion. The results of this study for the first time indicated that the endophytic fungus identified as O. brasiliensis MTCC 25236 isolated from the C. caesia rhizome could be a possible source for naturally producing calebin-A.

journal_name

AMB Express

journal_title

AMB Express

authors

Majeed A,Majeed M,Thajuddin N,Arumugam S,Ali F,Beede K,Adams SJ,Gnanamani M

doi

10.1186/s13568-019-0802-9

subject

Has Abstract

pub_date

2019-05-29 00:00:00

pages

79

issue

1

issn

2191-0855

pii

10.1186/s13568-019-0802-9

journal_volume

9

pub_type

杂志文章
  • First evaluation of alkylpyrazine application as a novel method to decrease microbial contaminations in processed meat products.

    abstract::Every year about 20% of the globally produced meat gets lost due to microbial spoilage. Nevertheless, the demand for processed meat is constantly rising and producers are searching for novel strategies to reduce microbial contaminations in their products. In the present study, we evaluated the applicability of alkylpy...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0583-6

    authors: Schöck M,Liebminger S,Berg G,Cernava T

    更新日期:2018-04-03 00:00:00

  • Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

    abstract::With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-4-7

    authors: Eibl JK,Corcoran JD,Senhorinho GN,Zhang K,Hosseini NS,Marsden J,Laamanen CA,Scott JA,Ross GM

    更新日期:2014-03-26 00:00:00

  • Autolysis of Pichia pastoris induced by cold.

    abstract::The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0397-y

    authors: Bartolo-Aguilar Y,Dendooven L,Chávez-Cabrera C,Flores-Cotera LB,Hidalgo-Lara ME,Villa-Tanaca L,Marsch R

    更新日期:2017-12-01 00:00:00

  • Construction of a novel Pichia pastoris strain for production of xanthophylls.

    abstract:: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-2-24

    authors: Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

    更新日期:2012-04-25 00:00:00

  • High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli.

    abstract::Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitiv...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01163-8

    authors: Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZL

    更新日期:2020-12-11 00:00:00

  • Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase.

    abstract::Clean coal technologies (e.g. coal biosolubilization) are of essential value, especially in Europe, where coal is the national wealth and other energy sources like crude oil are not available. Fusarium oxysporum LOCK 1134, the strain isolated from brown coal, efficiently biosolubilizes lignite. The obtained liquefied ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0669-1

    authors: Kwiatos N,Jędrzejczak-Krzepkowska M,Strzelecki B,Bielecki S

    更新日期:2018-08-22 00:00:00

  • Impacts of cerium oxide nanoparticles on bacterial community in activated sludge.

    abstract::Rapidly developing industry raises concerns about the environmental impacts of nanoparticles, but the effects of inorganic nanoparticles on bacterial community in wastewater treatment remain unclear. The present research assessed the impact of cerium oxide nanoparticles (nCeO) on the microbiome of activated sludge sys...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0365-6

    authors: Kamika I,Tekere M

    更新日期:2017-12-01 00:00:00

  • Design, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae.

    abstract::Helical alignment of the α-helical linker of the LOV (light-oxygen-voltage) domain of YtvA from Bacillus subtilis with the α-helical linker of the histidine-protein kinase domain of the Sln1 kinase of the phospho-relay system for osmoregulation of Saccharomyces cerevisiae has been used to construct a light-modulatable...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0582-7

    authors: Bury A,Hellingwerf KJ

    更新日期:2018-04-02 00:00:00

  • Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla.

    abstract::High-performance liquid chromatography-mass spectrometry (LC-MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0413-2

    authors: Gu F,Chen Y,Hong Y,Fang Y,Tan L

    更新日期:2017-12-01 00:00:00

  • Multi potent aromatic nano colloid: synthesis, characterization and applications.

    abstract::In this study the aromatic nanocolloids (CANCs) are synthesized using the noble metal silver by using Citronella extract and confirmed through physio chemical analysis. The synthesised CANCs were evaluated for its antimicrobial activity and antibiofilm activity against pathogenic biofilm forming E. coli. In addition, ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01104-5

    authors: Soundhararajan R,Mohamed Sheik Meeran SAF,Prakash SP,Mohammad W,Kandasamy R,Srinivasan H

    更新日期:2020-09-18 00:00:00

  • Recovery of platinum(0) through the reduction of platinum ions by hydrogenase-displaying yeast.

    abstract::Biological technologies for recycling rare metals, which are essential for high-tech products, have attracted much attention because they could prove to be more environmentally friendly and energy-saving than other methods. We have developed biological recycling technologies by cell surface engineering for the selecti...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0262-4

    authors: Ito R,Kuroda K,Hashimoto H,Ueda M

    更新日期:2016-12-01 00:00:00

  • Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare L. essential oil.

    abstract::Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0764-y

    authors: Fikry S,Khalil N,Salama O

    更新日期:2019-03-26 00:00:00

  • Identification of a bacteriocin-like compound from Lactobacillus plantarum with antimicrobial activity and effects on normal and cancerogenic human intestinal cells.

    abstract::In this paper, we demonstrate that the antimicrobial activity of L. plantarum PBS067 strain against antagonist microorganisms was mediated by the production of a bacteriocin-like compound secreted at the stationary phase of the growth. The novel bacteriocin-like compound, designed plantaricin P1053, was identified by ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0813-6

    authors: De Giani A,Bovio F,Forcella M,Fusi P,Sello G,Di Gennaro P

    更新日期:2019-06-17 00:00:00

  • Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.

    abstract::The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell b...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01160-x

    authors: Werner A,Otte KL,Stahlhut G,Pöggeler S

    更新日期:2020-12-21 00:00:00

  • A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities.

    abstract::Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify a...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0943-x

    authors: Wang Y,Zhao B,Liu Y,Mao L,Zhang X,Meng W,Liu K,Chu J

    更新日期:2020-01-14 00:00:00

  • Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).

    abstract::Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments. To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0652-x

    authors: Lyu T,Liu G,Zhang H,Wang L,Zhou S,Dou H,Pang B,Sha W,Zhang H

    更新日期:2018-08-02 00:00:00

  • Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii.

    abstract::Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0057-4

    authors: Almaraz-Delgado AL,Flores-Uribe J,Pérez-España VH,Salgado-Manjarrez E,Badillo-Corona JA

    更新日期:2014-08-13 00:00:00

  • Effects of rehydration nutrients on H2S metabolism and formation of volatile sulfur compounds by the wine yeast VL3.

    abstract:: In winemaking, nutrient supplementation is a common practice for optimising fermentation and producing quality wine. Nutritionally suboptimal grape juices are often enriched with nutrients in order to manipulate the production of yeast aroma compounds. Nutrients are also added to active dry yeast (ADY) rehydration me...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-1-36

    authors: Winter G,Henschke PA,Higgins VJ,Ugliano M,Curtin CD

    更新日期:2011-11-02 00:00:00

  • In vitro production of two chitinolytic proteins with an inhibiting effect on the insect coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) and the fungus Hemileia vastatrix the most limiting pests of coffee crops.

    abstract:: Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP). The goal was to produce and test proteins that inhibit both the coffee berry borer insect Hypothenemus ha...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-2-22

    authors: Martínez CP,Echeverri C,Florez JC,Gaitan AL,Góngora CE

    更新日期:2012-03-30 00:00:00

  • MicroRNA-466c-3p exerts protective effect on neuronal apoptosis and improves functional recovery post spinal cord injury via mitochondrial apoptotic pathway.

    abstract::Spinal cord injury (SCI) is involved with abnormal expression of miRNAs (miRs) which are responsible for some IIry injury responses which include apoptosis, inflammation and oxidative stress. Mechanisms involving miRs induced apoptosis still needs to be investigated. In the present work we developed a rat model of SCI...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01033-3

    authors: An Y,Li J,Yuan Q,Fan M

    更新日期:2020-06-15 00:00:00

  • Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20.

    abstract::Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01136-x

    authors: Liu Z,Dang K,Li C,Gao J,Wang H,Gao Y,Zhao B,Fan P,Qian A

    更新日期:2020-10-31 00:00:00

  • Silver ion bioreduction in nanoparticles using Artemisia annua L. extract: characterization and application as antibacterial agents.

    abstract::The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs functi...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01002-w

    authors: Aghajanyan A,Gabrielyan L,Schubert R,Trchounian A

    更新日期:2020-04-07 00:00:00

  • Contamination of water resources by pathogenic bacteria.

    abstract::Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. Increasing interest in controlling water-borne pathogens in water resources evidenced by a large number of recent publications clearly attests to the need for studies that synthesize knowl...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0051-x

    authors: Pandey PK,Kass PH,Soupir ML,Biswas S,Singh VP

    更新日期:2014-06-28 00:00:00

  • Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara).

    abstract::Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attemp...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01011-9

    authors: Watanabe Y,Kuroda K,Tatemichi Y,Nakahara T,Aoki W,Ueda M

    更新日期:2020-04-15 00:00:00

  • Importance of stress-response genes to the survival of airborne Escherichia coli under different levels of relative humidity.

    abstract::Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0376-3

    authors: Ng TW,Chan WL,Lai KM

    更新日期:2017-12-01 00:00:00

  • Complete conversion of all typical glycosylated protopanaxatriol ginsenosides to aglycon protopanaxatriol by combined bacterial β-glycosidases.

    abstract::Aglycon protopanaxatriol (APPT) has valuable pharmacological effects such as anti-inflammatory and anti-stress activities. However, the complete conversion of all typical glycosylated protopanaxatriol ginsenosides to APPT has not been achieved to date. β-Glycosidase from the hyperthermophilic bacterium Dictyoglomus tu...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0543-1

    authors: Yang EJ,Kim TH,Shin KC,Oh DK

    更新日期:2018-01-24 00:00:00

  • Natural antibacterial agents from arid-region pretreated lignocellulosic biomasses and extracts for the control of lactic acid bacteria in yeast fermentation.

    abstract::Bacterial contamination is one of the major challenges faced by yeast fermentation industries as the contaminating microorganisms produce lactic acid and acetic acid, which reduces the viability of yeast, and hence fermentation yields. The primary bacterial contaminants of yeast fermentations are lactic acid bacteria ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0654-8

    authors: Haris S,Fang C,Bastidas-Oyanedel JR,Prather KJ,Schmidt JE,Thomsen MH

    更新日期:2018-08-06 00:00:00

  • Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis.

    abstract::Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli ar...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0396-z

    authors: Mozejko-Ciesielska J,Dabrowska D,Szalewska-Palasz A,Ciesielski S

    更新日期:2017-12-01 00:00:00

  • Enhancement of active compound, genipin, from Gardeniae Fructus using immobilized glycosyl hydrolase family 3 β-glucosidase from Lactobacillus antri.

    abstract::Geniposide is an iridoid glycoside, which is abundant in Gardeniae Fructus. Despite the various pharmaceutical effects of geniposide on a human body, its hydrolysis into a smaller molecule, genipin, by β-glucosidase produced by bacteria in the intestines is particularly important to improve geniposide uptake into the ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0360-y

    authors: Kim YS,Lee CJ,Ma JY

    更新日期:2017-12-01 00:00:00

  • The regulatory effect of Lactobacillus rhamnosus GG on T lymphocyte and the development of intestinal villi in piglets of different periods.

    abstract::The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-00980-1

    authors: Shonyela SM,Feng B,Yang W,Yang G,Wang C

    更新日期:2020-04-17 00:00:00