Abstract:
:Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.
journal_name
AMB Expressjournal_title
AMB Expressauthors
Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZLdoi
10.1186/s13568-020-01163-8subject
Has Abstractpub_date
2020-12-11 00:00:00pages
214issue
1issn
2191-0855pii
10.1186/s13568-020-01163-8journal_volume
10pub_type
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