Complete conversion of all typical glycosylated protopanaxatriol ginsenosides to aglycon protopanaxatriol by combined bacterial β-glycosidases.

Abstract:

:Aglycon protopanaxatriol (APPT) has valuable pharmacological effects such as anti-inflammatory and anti-stress activities. However, the complete conversion of all typical glycosylated protopanaxatriol ginsenosides to APPT has not been achieved to date. β-Glycosidase from the hyperthermophilic bacterium Dictyoglomus turgidum (DT-bgl) hydrolyzes the glucose residues at C-6 and the inner glucose at C-20 in protopanaxatriol (PPT), but not the outer rhamnose residues at C-6. In contrast, β-glycosidase from the hyperthermophilic bacterium Pyrococcus furiosus (PF-bgl) hydrolyzes the outer rhamnose residue at C-6 but not the inner glucose residues at C-6 and C-20 in PPT. Thus, the combined use of DT-bgl and PF-bgl resulted in the complete the conversion of all typical glycosylated PPT ginsenosides, including R1, R2, Re, Rg1, Rg2, Rh1, Rf, F1, F3, and F5, to APPT. DT-bgl combined with PF-bgl completely hydrolyzed 1.0 mg ml-1 R1 and 1.0 mg ml-1 total PPT-type ginsenosides in Panax notoginseng root extract to 0.5 and 0.63 mg ml-1 APPT for 4 and 3 h, with molar conversions of 100% and productivities of 125 and 210 mg l-1 h-1, respectively. To the best of our knowledge, this is the first report of the complete conversion of all typical glycosylated PPT ginsenosides to APPT and the highest productivity of APPT obtained from ginseng extract achieved to date.

journal_name

AMB Express

journal_title

AMB Express

authors

Yang EJ,Kim TH,Shin KC,Oh DK

doi

10.1186/s13568-018-0543-1

subject

Has Abstract

pub_date

2018-01-24 00:00:00

pages

8

issue

1

issn

2191-0855

pii

10.1186/s13568-018-0543-1

journal_volume

8

pub_type

杂志文章
  • Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses.

    abstract::Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and fe...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01130-3

    authors: Li XJ,Wang M,Xue Y,Duan D,Li C,Han X,Wang K,Qiao R,Li XL

    更新日期:2020-10-29 00:00:00

  • Identification of a bacteriocin-like compound from Lactobacillus plantarum with antimicrobial activity and effects on normal and cancerogenic human intestinal cells.

    abstract::In this paper, we demonstrate that the antimicrobial activity of L. plantarum PBS067 strain against antagonist microorganisms was mediated by the production of a bacteriocin-like compound secreted at the stationary phase of the growth. The novel bacteriocin-like compound, designed plantaricin P1053, was identified by ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0813-6

    authors: De Giani A,Bovio F,Forcella M,Fusi P,Sello G,Di Gennaro P

    更新日期:2019-06-17 00:00:00

  • Importance of stress-response genes to the survival of airborne Escherichia coli under different levels of relative humidity.

    abstract::Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0376-3

    authors: Ng TW,Chan WL,Lai KM

    更新日期:2017-12-01 00:00:00

  • A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities.

    abstract::Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify a...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0943-x

    authors: Wang Y,Zhao B,Liu Y,Mao L,Zhang X,Meng W,Liu K,Chu J

    更新日期:2020-01-14 00:00:00

  • Effects of rehydration nutrients on H2S metabolism and formation of volatile sulfur compounds by the wine yeast VL3.

    abstract:: In winemaking, nutrient supplementation is a common practice for optimising fermentation and producing quality wine. Nutritionally suboptimal grape juices are often enriched with nutrients in order to manipulate the production of yeast aroma compounds. Nutrients are also added to active dry yeast (ADY) rehydration me...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-1-36

    authors: Winter G,Henschke PA,Higgins VJ,Ugliano M,Curtin CD

    更新日期:2011-11-02 00:00:00

  • Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions.

    abstract::Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box-Behnken design with three factors, the glucose and sodium acetate conce...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0180-5

    authors: Silva HR,Prete CEC,Zambrano F,de Mello VH,Tischer CA,Andrade DS

    更新日期:2016-03-01 00:00:00

  • Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus.

    abstract::The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternativ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0188-x

    authors: da Cruz Ramos GF,Ramos PL,Passarini MRZ,Vieira Silveira MA,Okamoto DN,de Oliveira LCG,Zezzo LV,Marem A,Santos Rocha RC,da Cruz JB,Juliano L,de Vasconcellos SP

    更新日期:2016-03-01 00:00:00

  • The regulatory effect of Lactobacillus rhamnosus GG on T lymphocyte and the development of intestinal villi in piglets of different periods.

    abstract::The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-00980-1

    authors: Shonyela SM,Feng B,Yang W,Yang G,Wang C

    更新日期:2020-04-17 00:00:00

  • Accessing inoculation methods of maize and wheat with Azospirillum brasilense.

    abstract::The utilization of inoculants containing Azospirillum is becoming more popular due to increasing reports of expressive gains in grain yields. However, incompatibility with pesticides used in seed treatments represents a main limitation for a successful inoculation. Therefore, in this study we searched for alternatives...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0171-y

    authors: Fukami J,Nogueira MA,Araujo RS,Hungria M

    更新日期:2016-03-01 00:00:00

  • Comparative metabolomics in vanilla pod and vanilla bean revealing the biosynthesis of vanillin during the curing process of vanilla.

    abstract::High-performance liquid chromatography-mass spectrometry (LC-MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0413-2

    authors: Gu F,Chen Y,Hong Y,Fang Y,Tan L

    更新日期:2017-12-01 00:00:00

  • Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing.

    abstract::The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01158-5

    authors: Hu Z,Ma D,Niu H,Chang J,Yu J,Tong Q,Li S

    更新日期:2021-01-06 00:00:00

  • Adaptive response of Rhodococcus opacus PWD4 to salt and phenolic stress on the level of mycolic acids.

    abstract::Mycolata form a group of Gram-positive bacteria with unique cell envelope structures that are known for their high tolerance against antibiotics and both aromatic and aliphatic hydrocarbons. An important part of the unique surface structure of the mycolata is the presence of long chain α-alkyl-β-hydroxy fatty acids, t...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0241-9

    authors: de Carvalho CCCR,Fischer MA,Kirsten S,Würz B,Wick LY,Heipieper HJ

    更新日期:2016-12-01 00:00:00

  • Chelatococcus thermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study.

    abstract::The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0209-9

    authors: Ibrahim MHA,Lebbe L,Willems A,Steinbüchel A

    更新日期:2016-12-01 00:00:00

  • Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20.

    abstract::Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01136-x

    authors: Liu Z,Dang K,Li C,Gao J,Wang H,Gao Y,Zhao B,Fan P,Qian A

    更新日期:2020-10-31 00:00:00

  • Multi potent aromatic nano colloid: synthesis, characterization and applications.

    abstract::In this study the aromatic nanocolloids (CANCs) are synthesized using the noble metal silver by using Citronella extract and confirmed through physio chemical analysis. The synthesised CANCs were evaluated for its antimicrobial activity and antibiofilm activity against pathogenic biofilm forming E. coli. In addition, ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01104-5

    authors: Soundhararajan R,Mohamed Sheik Meeran SAF,Prakash SP,Mohammad W,Kandasamy R,Srinivasan H

    更新日期:2020-09-18 00:00:00

  • Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration.

    abstract::The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was in three distinct growth stages: cell growth, lipid accumulation and a final lipid turnover stage. Most of ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0384-3

    authors: Safdar W,Shamoon M,Zan X,Haider J,Sharif HR,Shoaib M,Song Y

    更新日期:2017-12-01 00:00:00

  • The two-component system CepRS regulates the cephamycin C biosynthesis in Streptomyces clavuligerus F613-1.

    abstract::During industrial fermentation, Streptomyces clavuligerus F613-1 simultaneously produces primary product clavulanic acid (CA) and cephamycin C. The cephamycin C biosynthetic gene cluster and pathway have been basically elucidated and the CcaR positive regulator was found to control the cephamycin genes expression. How...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0844-z

    authors: Fu J,Qin R,Zong G,Zhong C,Zhang P,Kang N,Qi X,Cao G

    更新日期:2019-07-27 00:00:00

  • High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli.

    abstract::Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitiv...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01163-8

    authors: Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZL

    更新日期:2020-12-11 00:00:00

  • The biodiversity of Lactobacillus spp. from Iranian raw milk Motal cheese and antibacterial evaluation based on bacteriocin-encoding genes.

    abstract::Lactobacilli, as the largest group of lactic acid bacteria, produce large amounts of antimicrobial metabolites such as organic acids, fatty acids, ammonia, hydrogen peroxide, diacetyl and bacteriocin, which inhibit the growth of pathogenic bacteria and increase shelf life of food. The aim of this study was to identify...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0474-2

    authors: Azizi F,Habibi Najafi MB,Edalatian Dovom MR

    更新日期:2017-09-18 00:00:00

  • Metagenomic analysis reveals potential interactions in an artificial coculture.

    abstract::Disentangling the interactions between cyanobacteria and associated bacterial community is important for understanding the mechanisms that mediate the formation of cyanobacterial blooms in freshwater ecosystems. Despite the fact that a metagenomic approach enables researchers to profile the structure of microbial comm...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0490-2

    authors: Ren M,Zhang G,Ye Z,Qiao Z,Xie M,Lin Y,Li T,Zhao J

    更新日期:2017-11-02 00:00:00

  • Biosynthesis of uniformly carbon isotope-labeled docosahexaenoic acid in Crypthecodinium cohnii.

    abstract::Docosahexaenoic acid (DHA) enriched in brain can yield many important degradation products after the attack of hydroxyl radicals, which is known to serve as a nutraceutical and neuroprotective effects. Oxidative stress is a commonly observed feature of Alzheimer's disease (AD). Therefore, uniformly radiolabeled DHA pl...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-00981-0

    authors: Song P,Kuryatov A,Axelsen PH

    更新日期:2020-03-11 00:00:00

  • Ability of different edible fungi to degrade crop straw.

    abstract::Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liqui...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0731-z

    authors: Huang L,Sun N,Ban L,Wang Y,Yang H

    更新日期:2019-01-07 00:00:00

  • Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation.

    abstract::Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0127-2

    authors: Kitahara N,Morisaka H,Aoki W,Takeda Y,Shibasaki S,Kuroda K,Ueda M

    更新日期:2015-12-01 00:00:00

  • Construction of a novel Pichia pastoris strain for production of xanthophylls.

    abstract:: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-2-24

    authors: Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

    更新日期:2012-04-25 00:00:00

  • Long-term nitrate removal through methane-dependent denitrification microorganisms in sequencing batch reactors fed with only nitrate and methane.

    abstract::Denitrifying anaerobic methane oxidation (damo) bioprocesses can remove nitrate using methane as the electron donor, which gains great concern due to the current stringent discharge standard of nitrogen in wastewater treatment plants. To obtain an engineering acceptable nitrogen removal rate (NRR) and demonstrate the ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0637-9

    authors: Li W,Lu P,Chai F,Zhang L,Han X,Zhang D

    更新日期:2018-06-30 00:00:00

  • MicroRNA-143 targets MAPK3 to regulate the proliferation and bone metastasis of human breast cancer cells.

    abstract::MicroRNAs (miRs) have shown tremendous potential to act as therapeutic targets for cancer treatment. In this context, the present study was designed to investigate the potential of miR-143 in the treatment of breast cancer. Results showed that miR-143 to be significantly (P < 0.05) downregulated in breast cancer tissu...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01072-w

    authors: Du Y,Zhang J,Meng Y,Huang M,Yan W,Wu Z

    更新日期:2020-07-31 00:00:00

  • Optimisation of enzymatic hydrolysis of cassava peel to produce fermentable sugars.

    abstract::Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase a...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0146-z

    authors: Bayitse R,Hou X,Bjerre AB,Saalia FK

    更新日期:2015-12-01 00:00:00

  • Construction of a biodynamic model for Cry protein production studies.

    abstract::Mathematical models have been used from growth kinetic simulation to gen regulatory networks prediction for B. thuringiensis culture. However, this culture is a time dependent dynamic process where cells physiology suffers several changes depending on the changes in the cell environment. Therefore, through its culture...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0079-y

    authors: Navarro-Mtz AK,Pérez-Guevara F

    更新日期:2014-12-01 00:00:00

  • Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol.

    abstract::Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella living...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-3-69

    authors: Tajima T,Fuki K,Kataoka N,Kudou D,Nakashimada Y,Kato J

    更新日期:2013-12-05 00:00:00

  • Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora.

    abstract::A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl2 treatment of the protoplasts and plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by a homologous promoter. During re...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0818-1

    authors: Honda Y,Tanigawa E,Tsukihara T,Nguyen DX,Kawabe H,Sakatoku N,Watari J,Sato H,Yano S,Tachiki T,Irie T,Watanabe T,Watanabe T

    更新日期:2019-06-24 00:00:00