Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration.

abstract：:Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liqui...

journal_title：AMB Express

authors： Huang L,Sun N,Ban L,Wang Y,Yang H

更新日期：2019-01-07 00:00:00

abstract：:The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broa...

journal_title：AMB Express

authors： Ao XL,Yu X,Wu DT,Li C,Zhang T,Liu SL,Chen SJ,He L,Zhou K,Zou LK

更新日期：2018-06-12 00:00:00

abstract：:Green alga Chlamydomonas reinhardtii has gained interest as a sustainable resource because it can be easily grown using CO2 as a carbon source owing to its high CO2 assimilating activity. Although the robustness of the cell wall of C. reinhardtii makes it difficult to extract its intracellular products, such property ...

journal_title：AMB Express

authors： Nakanishi A,Iritani K,Sakihama Y,Ozawa N,Mochizuki A,Watanabe M

更新日期：2020-06-10 00:00:00

abstract：: Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP). The goal was to produce and test proteins that inhibit both the coffee berry borer insect Hypothenemus ha...

journal_title：AMB Express

authors： Martínez CP,Echeverri C,Florez JC,Gaitan AL,Góngora CE

更新日期：2012-03-30 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：:Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attemp...

journal_title：AMB Express

authors： Watanabe Y,Kuroda K,Tatemichi Y,Nakahara T,Aoki W,Ueda M

更新日期：2020-04-15 00:00:00

abstract：:Mathematical models have been used from growth kinetic simulation to gen regulatory networks prediction for B. thuringiensis culture. However, this culture is a time dependent dynamic process where cells physiology suffers several changes depending on the changes in the cell environment. Therefore, through its culture...

journal_title：AMB Express

authors： Navarro-Mtz AK,Pérez-Guevara F

更新日期：2014-12-01 00:00:00

abstract：:With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae...

journal_title：AMB Express

authors： Eibl JK,Corcoran JD,Senhorinho GN,Zhang K,Hosseini NS,Marsden J,Laamanen CA,Scott JA,Ross GM

更新日期：2014-03-26 00:00:00

abstract：:Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this ext...

journal_title：AMB Express

authors： Roberts DP,Selmer K,Lupitskyy R,Rice C,Buyer JS,Maul JE,Lakshman DK,DeSouza J

更新日期：2021-01-06 00:00:00

abstract：:During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the S...

journal_title：AMB Express

authors： El-Rotail AAMM,Zhang L,Li Y,Liu SP,Shi GY

更新日期：2017-12-01 00:00:00

abstract：:Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

journal_title：AMB Express

authors： Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

更新日期：2020-02-25 00:00:00

abstract：:The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs functi...

journal_title：AMB Express

authors： Aghajanyan A,Gabrielyan L,Schubert R,Trchounian A

更新日期：2020-04-07 00:00:00

abstract：:Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel ...

journal_title：AMB Express

authors： Sakai K,Yamaguchi A,Tsutsumi S,Kawai Y,Tsuzuki S,Suzuki H,Jindou S,Suzuki Y,Kajimura H,Kato M,Shimizu M

更新日期：2020-05-24 00:00:00

abstract：:The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...

journal_title：AMB Express

authors： Ibrahim MHA,Lebbe L,Willems A,Steinbüchel A

更新日期：2016-12-01 00:00:00

abstract：:The discovery of aerobic and anammox bacteria capable of generating methane in bio-filters in freshwater aquaculture systems is generating interest in studies to understand the activity, diversity, distribution and roles of these environmental bacteria. In this study, we used microbial enrichment of bio-filters to ass...

journal_title：AMB Express

authors： Kamira B,Shi LL,Fan LM,Zhang C,Zheng Y,Song C,Meng SL,Hu GD,Bing XW,Chen ZJ,Xu P

更新日期：2018-08-28 00:00:00

abstract：:Sponges are primitive metazoans that are known to harbour diverse and abundant microbes. All over the world attempts are being made to exploit these microbes for their biotechnological potential to produce, bioactive compounds and antimicrobial peptides. However, the majority of the studies are focussed on the marine ...

journal_title：AMB Express

authors： Gaikwad S,Shouche YS,Gade WN

更新日期：2016-12-01 00:00:00

abstract：:Trace metals are required in many cellular processes in bacteria but also induce toxic effects to cells when present in excess. As such, various forms of adaptive responses towards extracellular trace metal ions are essential for the survival and fitness of bacteria in their environment. A soil Pseudomonas putida, str...

journal_title：AMB Express

authors： Chong TM,Yin WF,Chen JW,Mondy S,Grandclément C,Faure D,Dessaux Y,Chan KG

更新日期：2016-12-01 00:00:00

abstract：: Biocathode MFCs using microorganisms as catalysts have important advantages in lowering cost and improving sustainability. Electrode materials and microbial synergy determines biocathode MFCs performance. In this study, four materials, granular activated carbon (GAC), granular semicoke (GS), granular graphite (GG) an...

journal_title：AMB Express

authors： Sun Y,Wei J,Liang P,Huang X

更新日期：2012-03-29 00:00:00

abstract：:Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. Lipophilic dyes such as Nile red and BODIPY have been used to quantify intracellular PHB, but their uses have often been limited in...

journal_title：AMB Express

authors： Choi JE,Na HY,Yang TH,Rhee SK,Song JK

更新日期：2015-12-01 00:00:00

abstract：:Bacillus thuringiensis is a Gram-positive soil bacterium that is known to be a bacterial biopesticide that produces insecticidal proteins called crystal proteins (Cry). In the insecticidal process, chitinases are suggested to perforate the peritrophic membrane barrier to facilitate the invasion of the Cry proteins int...

journal_title：AMB Express

authors： Honda S,Kunii T,Nohara K,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

更新日期：2017-12-01 00:00:00

abstract：:Sweet potato vine (SPV) is an abundant agricultural waste, which is easy to obtain at low cost and has the potential to produce clean energy via anaerobic digestion (AD). The main objectives of this study were to reveal methane production and process stability of SPV and the mixtures with animal manure under various t...

journal_title：AMB Express

authors： Zhang E,Li J,Zhang K,Wang F,Yang H,Zhi S,Liu G

更新日期：2018-03-22 00:00:00

abstract：:Phasins are amphiphilic proteins involved in the regulation of the number and size of polyhydroxybutyrate (PHB) granules. The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and energy source. By analyzing the genome, we identified six genes that cod...

journal_title：AMB Express

authors： Martínez-Martínez MLA,González-Pedrajo B,Dreyfus G,Soto-Urzúa L,Martínez-Morales LJ

更新日期：2019-09-25 00:00:00

abstract：:Herbicides are important tools for weed control in modern agriculture. In the search for potential herbicidal natural products from fungal species, harzianum A and B were identified from the biofertilizer fungus, Trichoderma brevicompactum. In the phytotoxicity assays on the dicot species Brassica chinensis, harzianum...

journal_title：AMB Express

authors： Yin M,Fasoyin OE,Wang C,Yue Q,Zhang Y,Dun B,Xu Y,Zhang L

更新日期：2020-07-01 00:00:00

abstract：:Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitiv...

journal_title：AMB Express

authors： Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZL

更新日期：2020-12-11 00:00:00

abstract：:Disentangling the interactions between cyanobacteria and associated bacterial community is important for understanding the mechanisms that mediate the formation of cyanobacterial blooms in freshwater ecosystems. Despite the fact that a metagenomic approach enables researchers to profile the structure of microbial comm...

journal_title：AMB Express

authors： Ren M,Zhang G,Ye Z,Qiao Z,Xie M,Lin Y,Li T,Zhao J

更新日期：2017-11-02 00:00:00

abstract：:Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concent...

journal_title：AMB Express

authors： Chen D,Wang Y,Chen M,Fan P,Li G,Wang C

更新日期：2021-01-04 00:00:00

abstract：:Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressu...

journal_title：AMB Express

authors： Sakudo A,Yagyu Y

更新日期：2021-01-09 00:00:00

abstract：:Thermomonospora curvata is a thermophilic actinomycete phylogenetically related to Thermobifida fusca that produces extracellular hydrolases capable of degrading synthetic polyesters. Analysis of the genome of T. curvata DSM43183 revealed two genes coding for putative polyester hydrolases Tcur1278 and Tcur0390 sharing...

journal_title：AMB Express

authors： Wei R,Oeser T,Then J,Kühn N,Barth M,Schmidt J,Zimmermann W

更新日期：2014-06-03 00:00:00

abstract：: Vinasse, a highly polluting waste of the ethanol industry was utilized for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloferax mediterranei in shake-flasks. Following pre-treatment through adsorption on activated carbon, 25%-50% (v/v) pre-treated vinasse was utilized leading t...

journal_title：AMB Express

authors： Bhattacharyya A,Pramanik A,Maji SK,Haldar S,Mukhopadhyay UK,Mukherjee J

更新日期：2012-07-09 00:00:00

abstract：:In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB', gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been heterologously expressed in Escherichia coli, purified as protein possessing a His-Tag and initially characterized. FadB' is...

journal_title：AMB Express

authors： Volodina E,Steinbüchel A

更新日期：2014-08-28 00:00:00