Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle.

Abstract:

:Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel excavated by Euwallacea interjectus and isolated the cellulose-degrading fungus, Fusarium spp. strain EI, by enrichment culture with carboxymethyl cellulose as the sole carbon source. The cellulolytic enzyme secreted by the fungus was identified and expressed in Pichia pastoris, and its enzymatic properties were characterized. The cellulolytic enzyme, termed FsXEG12A, could hydrolyze carboxymethyl cellulose, microcrystalline cellulose, xyloglucan, lichenan, and glucomannan, indicating that the broad substrate specificity of FsXEG12A could be beneficial for degrading complex wood components such as cellulose, xyloglucan, and galactoglucomannan in angiosperms. Inhibition of FsXEG12A function is, thus, an effective target for Fusarium dieback caused by Euwallacea spp.

journal_name

AMB Express

journal_title

AMB Express

authors

Sakai K,Yamaguchi A,Tsutsumi S,Kawai Y,Tsuzuki S,Suzuki H,Jindou S,Suzuki Y,Kajimura H,Kato M,Shimizu M

doi

10.1186/s13568-020-01030-6

subject

Has Abstract

pub_date

2020-05-24 00:00:00

pages

96

issue

1

issn

2191-0855

pii

10.1186/s13568-020-01030-6

journal_volume

10

pub_type

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