Abstract:
:Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel excavated by Euwallacea interjectus and isolated the cellulose-degrading fungus, Fusarium spp. strain EI, by enrichment culture with carboxymethyl cellulose as the sole carbon source. The cellulolytic enzyme secreted by the fungus was identified and expressed in Pichia pastoris, and its enzymatic properties were characterized. The cellulolytic enzyme, termed FsXEG12A, could hydrolyze carboxymethyl cellulose, microcrystalline cellulose, xyloglucan, lichenan, and glucomannan, indicating that the broad substrate specificity of FsXEG12A could be beneficial for degrading complex wood components such as cellulose, xyloglucan, and galactoglucomannan in angiosperms. Inhibition of FsXEG12A function is, thus, an effective target for Fusarium dieback caused by Euwallacea spp.
journal_name
AMB Expressjournal_title
AMB Expressauthors
Sakai K,Yamaguchi A,Tsutsumi S,Kawai Y,Tsuzuki S,Suzuki H,Jindou S,Suzuki Y,Kajimura H,Kato M,Shimizu Mdoi
10.1186/s13568-020-01030-6subject
Has Abstractpub_date
2020-05-24 00:00:00pages
96issue
1issn
2191-0855pii
10.1186/s13568-020-01030-6journal_volume
10pub_type
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