Purification and characterization of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3.

abstract：:Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. Lipophilic dyes such as Nile red and BODIPY have been used to quantify intracellular PHB, but their uses have often been limited in...

journal_title：AMB Express

authors： Choi JE,Na HY,Yang TH,Rhee SK,Song JK

更新日期：2015-12-01 00:00:00

abstract：:The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternativ...

journal_title：AMB Express

authors： da Cruz Ramos GF,Ramos PL,Passarini MRZ,Vieira Silveira MA,Okamoto DN,de Oliveira LCG,Zezzo LV,Marem A,Santos Rocha RC,da Cruz JB,Juliano L,de Vasconcellos SP

更新日期：2016-03-01 00:00:00

abstract：:An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experiment...

journal_title：AMB Express

authors： Abdelrazek NA,Elkhatib WF,Raafat MM,Aboulwafa MM

更新日期：2020-04-15 00:00:00

abstract：:Mycolata form a group of Gram-positive bacteria with unique cell envelope structures that are known for their high tolerance against antibiotics and both aromatic and aliphatic hydrocarbons. An important part of the unique surface structure of the mycolata is the presence of long chain α-alkyl-β-hydroxy fatty acids, t...

journal_title：AMB Express

authors： de Carvalho CCCR,Fischer MA,Kirsten S,Würz B,Wick LY,Heipieper HJ

更新日期：2016-12-01 00:00:00

abstract：:Sweet potato vine (SPV) is an abundant agricultural waste, which is easy to obtain at low cost and has the potential to produce clean energy via anaerobic digestion (AD). The main objectives of this study were to reveal methane production and process stability of SPV and the mixtures with animal manure under various t...

journal_title：AMB Express

authors： Zhang E,Li J,Zhang K,Wang F,Yang H,Zhi S,Liu G

更新日期：2018-03-22 00:00:00

abstract：:Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L...

journal_title：AMB Express

authors： Kang TS,Korber DR,Tanaka T

更新日期：2013-01-31 00:00:00

abstract：:Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase a...

journal_title：AMB Express

authors： Bayitse R,Hou X,Bjerre AB,Saalia FK

更新日期：2015-12-01 00:00:00

abstract：:Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the alkali extraction process, xylanase and arabinofuranosidase are mainly used to hydrolyze xylooligosacc...

journal_title：AMB Express

authors： Zhang Y,Yang H,Yu X,Kong H,Chen J,Luo H,Bai Y,Yao B

更新日期：2019-01-29 00:00:00

abstract：:The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in this biotransformation. The complete hydrolysis of 5-caffeoylquinic acid (5-CQA) into caffeic acid (CA) by L...

journal_title：AMB Express

authors： Bel-Rhlid R,Thapa D,Kraehenbuehl K,Hansen CE,Fischer L

更新日期：2013-05-21 00:00:00

abstract：:Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel ...

journal_title：AMB Express

authors： Sakai K,Yamaguchi A,Tsutsumi S,Kawai Y,Tsuzuki S,Suzuki H,Jindou S,Suzuki Y,Kajimura H,Kato M,Shimizu M

更新日期：2020-05-24 00:00:00

abstract：:Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. Increasing interest in controlling water-borne pathogens in water resources evidenced by a large number of recent publications clearly attests to the need for studies that synthesize knowl...

journal_title：AMB Express

authors： Pandey PK,Kass PH,Soupir ML,Biswas S,Singh VP

更新日期：2014-06-28 00:00:00

abstract：:The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell b...

journal_title：AMB Express

authors： Werner A,Otte KL,Stahlhut G,Pöggeler S

更新日期：2020-12-21 00:00:00

abstract：:Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5...

journal_title：AMB Express

authors： Duarte JC,Valença GP,Moran PJ,Rodrigues JA

更新日期：2015-02-20 00:00:00

abstract：:In the present work, a remediation bioprocess based on the use of a native isolate of Chlorella vulgaris immobilized in an alginate matrix inside a polylactic acid (PLA) device is proposed. This microalga immobilized in alginate beads was previously shown to be useful for the reduction of several chemical and microbia...

journal_title：AMB Express

authors： Marconi PL,Trentini A,Zawoznik M,Nadra C,Mercadé JM,Sánchez Novoa JG,Orozco D,Groppa MD

更新日期：2020-08-15 00:00:00

abstract：:The effects of inlet VOCs (Volatile Organic Compounds) shifts on microbial community structure in a biofiltration system were investigated. A lab-scale biofilter was set up to treat eight VOCs sequentially. Short declines in removal efficiency appeared after VOCs shifts and then later recovered. The number of OTUs in ...

journal_title：AMB Express

authors： Lu L,Wang G,Yeung M,Xi J,Hu HY

更新日期：2018-10-03 00:00:00

abstract：:Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box-Behnken design with three factors, the glucose and sodium acetate conce...

journal_title：AMB Express

authors： Silva HR,Prete CEC,Zambrano F,de Mello VH,Tischer CA,Andrade DS

更新日期：2016-03-01 00:00:00

abstract：:Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this ext...

journal_title：AMB Express

authors： Roberts DP,Selmer K,Lupitskyy R,Rice C,Buyer JS,Maul JE,Lakshman DK,DeSouza J

更新日期：2021-01-06 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：:The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes...

journal_title：AMB Express

authors： Bartolo-Aguilar Y,Dendooven L,Chávez-Cabrera C,Flores-Cotera LB,Hidalgo-Lara ME,Villa-Tanaca L,Marsch R

更新日期：2017-12-01 00:00:00

abstract：:The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is comparatively simple, well characterized and can be manipulated easily...

journal_title：AMB Express

authors： Kangwa M,Yelemane V,Polat AN,Gorrepati KD,Grasselli M,Fernández-Lahore M

更新日期：2015-12-01 00:00:00

abstract：:Every year about 20% of the globally produced meat gets lost due to microbial spoilage. Nevertheless, the demand for processed meat is constantly rising and producers are searching for novel strategies to reduce microbial contaminations in their products. In the present study, we evaluated the applicability of alkylpy...

journal_title：AMB Express

authors： Schöck M,Liebminger S,Berg G,Cernava T

更新日期：2018-04-03 00:00:00

abstract：:Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli ar...

journal_title：AMB Express

authors： Mozejko-Ciesielska J,Dabrowska D,Szalewska-Palasz A,Ciesielski S

更新日期：2017-12-01 00:00:00

abstract：:Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

journal_title：AMB Express

authors： Ng TW,Chan WL,Lai KM

更新日期：2017-12-01 00:00:00

abstract：:In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over a...

journal_title：AMB Express

authors： Ji JH,Zhou L,Mbadinga SM,Irfan M,Liu YF,Pan P,Qi ZZ,Chen J,Liu JF,Yang SZ,Gu JD,Mu BZ

更新日期：2020-02-01 00:00:00

abstract：: To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-bu...

journal_title：AMB Express

authors： Koitabashi M,Noguchi MT,Sameshima-Yamashita Y,Hiradate S,Suzuki K,Yoshida S,Watanabe T,Shinozaki Y,Tsushima S,Kitamoto HK

更新日期：2012-08-02 00:00:00

abstract：:Mathematical models have been used from growth kinetic simulation to gen regulatory networks prediction for B. thuringiensis culture. However, this culture is a time dependent dynamic process where cells physiology suffers several changes depending on the changes in the cell environment. Therefore, through its culture...

journal_title：AMB Express

authors： Navarro-Mtz AK,Pérez-Guevara F

更新日期：2014-12-01 00:00:00

abstract：:Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments. To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes ...

journal_title：AMB Express

authors： Lyu T,Liu G,Zhang H,Wang L,Zhou S,Dou H,Pang B,Sha W,Zhang H

更新日期：2018-08-02 00:00:00

abstract：:Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

journal_title：AMB Express

authors： Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

更新日期：2020-02-25 00:00:00

abstract：:Disentangling the interactions between cyanobacteria and associated bacterial community is important for understanding the mechanisms that mediate the formation of cyanobacterial blooms in freshwater ecosystems. Despite the fact that a metagenomic approach enables researchers to profile the structure of microbial comm...

journal_title：AMB Express

authors： Ren M,Zhang G,Ye Z,Qiao Z,Xie M,Lin Y,Li T,Zhao J

更新日期：2017-11-02 00:00:00

abstract：:In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB', gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been heterologously expressed in Escherichia coli, purified as protein possessing a His-Tag and initially characterized. FadB' is...

journal_title：AMB Express

authors： Volodina E,Steinbüchel A

更新日期：2014-08-28 00:00:00