Phasin PhaP1 is involved in polyhydroxybutyrate granules morphology and in controlling early biopolymer accumulation in Azospirillum brasilense Sp7.

Abstract:

:Phasins are amphiphilic proteins involved in the regulation of the number and size of polyhydroxybutyrate (PHB) granules. The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and energy source. By analyzing the genome, we identified six genes that code for proteins with a Phasin_2 domain. To understand the role of A. brasilense Sp7 PhaP1 (PhaP1Abs) on PHB synthesis, the phaP1 gene (AMK58_RS17065) was deleted. The morphology of the PHB granules was analyzed by transmission electron microscopy (TEM) and the PHB produced was quantified under three different C:N ratios in cultures subjected to null or low-oxygen transfer. The results showed that PhaP1Abs is involved in PHB granules morphology and in controlling early biopolymer accumulation. Using RT-PCR it was found that phasin genes, except phaP4, are transcribed in accordance with the C:N ratio used for the growth of A. brasilense. phaP1, phaP2 and phaP3 genes were able to respond to the growth conditions tested. This study reports the first analysis of a phasin protein in A. brasilense Sp7.

journal_name

AMB Express

journal_title

AMB Express

authors

Martínez-Martínez MLA,González-Pedrajo B,Dreyfus G,Soto-Urzúa L,Martínez-Morales LJ

doi

10.1186/s13568-019-0876-4

subject

Has Abstract

pub_date

2019-09-25 00:00:00

pages

155

issue

1

issn

2191-0855

pii

10.1186/s13568-019-0876-4

journal_volume

9

pub_type

杂志文章
  • Identification of a bacteriocin-like compound from Lactobacillus plantarum with antimicrobial activity and effects on normal and cancerogenic human intestinal cells.

    abstract::In this paper, we demonstrate that the antimicrobial activity of L. plantarum PBS067 strain against antagonist microorganisms was mediated by the production of a bacteriocin-like compound secreted at the stationary phase of the growth. The novel bacteriocin-like compound, designed plantaricin P1053, was identified by ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0813-6

    authors: De Giani A,Bovio F,Forcella M,Fusi P,Sello G,Di Gennaro P

    更新日期:2019-06-17 00:00:00

  • Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare L. essential oil.

    abstract::Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0764-y

    authors: Fikry S,Khalil N,Salama O

    更新日期:2019-03-26 00:00:00

  • Changes in feeding habits promoted the differentiation of the composition and function of gut microbiotas between domestic dogs (Canis lupus familiaris) and gray wolves (Canis lupus).

    abstract::Wolves (Canis lupus) and their domesticated and close relatives, dogs (Canis lupus familiaris), have great differences in their diets and living environments. To the best of our knowledge, the fundamental question of how the abundance and function of the gut microbiota of domestic dogs evolved to adapt to the changes ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0652-x

    authors: Lyu T,Liu G,Zhang H,Wang L,Zhou S,Dou H,Pang B,Sha W,Zhang H

    更新日期:2018-08-02 00:00:00

  • A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production.

    abstract::During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the S...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0400-7

    authors: El-Rotail AAMM,Zhang L,Li Y,Liu SP,Shi GY

    更新日期:2017-12-01 00:00:00

  • Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20.

    abstract::Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01136-x

    authors: Liu Z,Dang K,Li C,Gao J,Wang H,Gao Y,Zhao B,Fan P,Qian A

    更新日期:2020-10-31 00:00:00

  • Multi potent aromatic nano colloid: synthesis, characterization and applications.

    abstract::In this study the aromatic nanocolloids (CANCs) are synthesized using the noble metal silver by using Citronella extract and confirmed through physio chemical analysis. The synthesised CANCs were evaluated for its antimicrobial activity and antibiofilm activity against pathogenic biofilm forming E. coli. In addition, ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01104-5

    authors: Soundhararajan R,Mohamed Sheik Meeran SAF,Prakash SP,Mohammad W,Kandasamy R,Srinivasan H

    更新日期:2020-09-18 00:00:00

  • Response of microbial community structure and metabolic profile to shifts of inlet VOCs in a gas-phase biofilter.

    abstract::The effects of inlet VOCs (Volatile Organic Compounds) shifts on microbial community structure in a biofiltration system were investigated. A lab-scale biofilter was set up to treat eight VOCs sequentially. Short declines in removal efficiency appeared after VOCs shifts and then later recovered. The number of OTUs in ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0687-z

    authors: Lu L,Wang G,Yeung M,Xi J,Hu HY

    更新日期:2018-10-03 00:00:00

  • NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level.

    abstract::A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0081-4

    authors: Knudsen JD,Carlquist M,Gorwa-Grauslund M

    更新日期:2014-10-30 00:00:00

  • Bifidogenic and butyrogenic effects of young barely leaf extract in an in vitro human colonic microbiota model.

    abstract::Young barley leaf extract (YBL) contains beneficial substances such as fructans, minerals, and vitamins. The effects of YBL administration on the human colonic microbiota and its production of metabolites were evaluated using an in vitro model culture system. Fermentations were started by inoculating fecal samples fro...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0911-5

    authors: Sasaki D,Sasaki K,Kadowaki Y,Aotsuka Y,Kondo A

    更新日期:2019-11-13 00:00:00

  • Genetic algorithm with a crossover elitist preservation mechanism for protein-ligand docking.

    abstract::Protein-ligand docking plays an important role in computer-aided pharmaceutical development. Protein-ligand docking can be defined as a search algorithm with a scoring function, whose aim is to determine the conformation of the ligand and the receptor with the lowest energy. Hence, to improve an efficient algorithm ha...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0476-0

    authors: Guan B,Zhang C,Ning J

    更新日期:2017-09-13 00:00:00

  • (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB') from fatty acid degradation operon of Ralstonia eutropha H16.

    abstract::In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB', gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been heterologously expressed in Escherichia coli, purified as protein possessing a His-Tag and initially characterized. FadB' is...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0069-0

    authors: Volodina E,Steinbüchel A

    更新日期:2014-08-28 00:00:00

  • A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities.

    abstract::Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify a...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0943-x

    authors: Wang Y,Zhao B,Liu Y,Mao L,Zhang X,Meng W,Liu K,Chu J

    更新日期:2020-01-14 00:00:00

  • Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions.

    abstract::Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box-Behnken design with three factors, the glucose and sodium acetate conce...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0180-5

    authors: Silva HR,Prete CEC,Zambrano F,de Mello VH,Tischer CA,Andrade DS

    更新日期:2016-03-01 00:00:00

  • Microbial community structure of two freshwater sponges using Illumina MiSeq sequencing revealed high microbial diversity.

    abstract::Sponges are primitive metazoans that are known to harbour diverse and abundant microbes. All over the world attempts are being made to exploit these microbes for their biotechnological potential to produce, bioactive compounds and antimicrobial peptides. However, the majority of the studies are focussed on the marine ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0211-2

    authors: Gaikwad S,Shouche YS,Gade WN

    更新日期:2016-12-01 00:00:00

  • Chelatococcus thermostellatus sp. nov., a new thermophile for bioplastic synthesis: comparative phylogenetic and physiological study.

    abstract::The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0209-9

    authors: Ibrahim MHA,Lebbe L,Willems A,Steinbüchel A

    更新日期:2016-12-01 00:00:00

  • Genomic insights into a robust gamma-aminobutyric acid-producer Lactobacillus brevis CD0817.

    abstract::Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0799-0

    authors: Gao D,Chang K,Ding G,Wu H,Chen Y,Jia M,Liu X,Wang S,Jin Y,Pan H,Li H

    更新日期:2019-05-24 00:00:00

  • Construction of a novel Pichia pastoris strain for production of xanthophylls.

    abstract:: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-2-24

    authors: Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

    更新日期:2012-04-25 00:00:00

  • Assessment of packed bed bioreactor systems in the production of viral vaccines.

    abstract::Vaccination is believed to be the most effective method for the prevention of infectious diseases. Thus it is imperative to develop cost effective and scalable process for the production of vaccines so as to make them affordable for mass use. In this study, performance of a novel disposable iCELLis fixed bed bioreacto...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0025-z

    authors: Rajendran R,Lingala R,Vuppu SK,Bandi BO,Manickam E,Macherla SR,Dubois S,Havelange N,Maithal K

    更新日期:2014-04-25 00:00:00

  • Comprehensive genomic and phenotypic metal resistance profile of Pseudomonas putida strain S13.1.2 isolated from a vineyard soil.

    abstract::Trace metals are required in many cellular processes in bacteria but also induce toxic effects to cells when present in excess. As such, various forms of adaptive responses towards extracellular trace metal ions are essential for the survival and fitness of bacteria in their environment. A soil Pseudomonas putida, str...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0269-x

    authors: Chong TM,Yin WF,Chen JW,Mondy S,Grandclément C,Faure D,Dessaux Y,Chan KG

    更新日期:2016-12-01 00:00:00

  • Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants.

    abstract:: To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-bu...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-2-40

    authors: Koitabashi M,Noguchi MT,Sameshima-Yamashita Y,Hiradate S,Suzuki K,Yoshida S,Watanabe T,Shinozaki Y,Tsushima S,Kitamoto HK

    更新日期:2012-08-02 00:00:00

  • A lipophilic fluorescent LipidGreen1-based quantification method for high-throughput screening analysis of intracellular poly-3-hydroxybutyrate.

    abstract::Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. Lipophilic dyes such as Nile red and BODIPY have been used to quantify intracellular PHB, but their uses have often been limited in...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0131-6

    authors: Choi JE,Na HY,Yang TH,Rhee SK,Song JK

    更新日期:2015-12-01 00:00:00

  • Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera.

    abstract::The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01143-y

    authors: Kaur N,Arora DS

    更新日期:2020-11-11 00:00:00

  • Therapeutic efficacy of catalpol against apoptosis in cardiomyocytes derived from human induced pluripotent stem cells.

    abstract::Cardiac arrhythmia is an irregular heart rhythm that can lead to serious heart conditions and various organ disorders, and may cause sudden cardiac death. Catalpol belongs to the iridoid glycoside family and is highly abundant in Rehmannia glutinosa Libosch. The study included five groups such as group I (normal contr...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-00986-9

    authors: Yang L,Feng X,Li Y,Zhang S,Ying Y

    更新日期:2020-03-20 00:00:00

  • Impacts of cerium oxide nanoparticles on bacterial community in activated sludge.

    abstract::Rapidly developing industry raises concerns about the environmental impacts of nanoparticles, but the effects of inorganic nanoparticles on bacterial community in wastewater treatment remain unclear. The present research assessed the impact of cerium oxide nanoparticles (nCeO) on the microbiome of activated sludge sys...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0365-6

    authors: Kamika I,Tekere M

    更新日期:2017-12-01 00:00:00

  • Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation.

    abstract::Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0127-2

    authors: Kitahara N,Morisaka H,Aoki W,Takeda Y,Shibasaki S,Kuroda K,Ueda M

    更新日期:2015-12-01 00:00:00

  • Ammonium nitrate regulated the color characteristic changes of pigments in Monascus purpureus M9.

    abstract::Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concent...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01165-6

    authors: Chen D,Wang Y,Chen M,Fan P,Li G,Wang C

    更新日期:2021-01-04 00:00:00

  • Construction of a biodynamic model for Cry protein production studies.

    abstract::Mathematical models have been used from growth kinetic simulation to gen regulatory networks prediction for B. thuringiensis culture. However, this culture is a time dependent dynamic process where cells physiology suffers several changes depending on the changes in the cell environment. Therefore, through its culture...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0079-y

    authors: Navarro-Mtz AK,Pérez-Guevara F

    更新日期:2014-12-01 00:00:00

  • Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli.

    abstract::As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0483-1

    authors: Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

    更新日期:2017-09-29 00:00:00

  • Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris.

    abstract::Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-00972-1

    authors: Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

    更新日期:2020-02-25 00:00:00

  • Bioconversion of curcumin into calebin-A by the endophytic fungus Ovatospora brasiliensis EPE-10 MTCC 25236 associated with Curcuma caesia.

    abstract::Calebin-A is a curcuminoid compound reported to be present in Curcuma longa rhizome. The current study was aimed to isolate and characterize calebin-A from Curcuma caesia rhizome and its production through biotransformation approach using endophytic fungus. C. caesia rhizomes of different ages were subjected to analys...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0802-9

    authors: Majeed A,Majeed M,Thajuddin N,Arumugam S,Ali F,Beede K,Adams SJ,Gnanamani M

    更新日期:2019-05-29 00:00:00